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Evaluation of the protective effect of sunscreens on in vitro reconstructed human skin exposed to UVB or UVA irradiation.
Photochem Photobiol. 2000 Mar; 71(3):314-20.PP

Abstract

We have previously shown that skin reconstructed in vitro is a useful model to study the effects of UVB and UVA exposure. Wavelength-specific biological damage has been identified such as the formation of sunburn cells (SBC) and pyrimidine dimers after UVB irradiation and alterations of dermal fibroblasts after UVA exposure. These specific effects were selected to evaluate the protection afforded by two sunscreens after topical application on the skin surface. Simplified formulations having different absorption spectra but similar sun protection factors were used. One contained a classical UVB absorber, 2-ethylhexyl-p-methoxycinnamate. The other contained a broad-spectrum absorber called Mexoryl SX, characterized by its strong absorbing potency in the UVA range. Both filters were used at 5% in a simple water/oil vehicle. The evaluation of photoprotection on in vitro reconstructed skin revealed good efficiency for both preparations in preventing UVB-induced damage, as shown by SBC counting and pyrimidine dimer immunostaining. By contrast, only the Mexoryl SX-containing preparation was able to efficiently prevent UVA-specific damage such as dermal fibroblast disappearance. Our data further support the fact that skin reconstructed in vitro is a reliable system to evaluate the photoprotection provided by different sunscreens against specific UVB and UVA biological damage.

Authors+Show Affiliations

L'Oréal, Life Sciences Research, Clichy, France. fbernerd@loreal-recherche.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

10732449

Citation

Bernerd, F, et al. "Evaluation of the Protective Effect of Sunscreens On in Vitro Reconstructed Human Skin Exposed to UVB or UVA Irradiation." Photochemistry and Photobiology, vol. 71, no. 3, 2000, pp. 314-20.
Bernerd F, Vioux C, Asselineau D. Evaluation of the protective effect of sunscreens on in vitro reconstructed human skin exposed to UVB or UVA irradiation. Photochem Photobiol. 2000;71(3):314-20.
Bernerd, F., Vioux, C., & Asselineau, D. (2000). Evaluation of the protective effect of sunscreens on in vitro reconstructed human skin exposed to UVB or UVA irradiation. Photochemistry and Photobiology, 71(3), 314-20.
Bernerd F, Vioux C, Asselineau D. Evaluation of the Protective Effect of Sunscreens On in Vitro Reconstructed Human Skin Exposed to UVB or UVA Irradiation. Photochem Photobiol. 2000;71(3):314-20. PubMed PMID: 10732449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the protective effect of sunscreens on in vitro reconstructed human skin exposed to UVB or UVA irradiation. AU - Bernerd,F, AU - Vioux,C, AU - Asselineau,D, PY - 2000/3/25/pubmed PY - 2000/5/8/medline PY - 2000/3/25/entrez SP - 314 EP - 20 JF - Photochemistry and photobiology JO - Photochem Photobiol VL - 71 IS - 3 N2 - We have previously shown that skin reconstructed in vitro is a useful model to study the effects of UVB and UVA exposure. Wavelength-specific biological damage has been identified such as the formation of sunburn cells (SBC) and pyrimidine dimers after UVB irradiation and alterations of dermal fibroblasts after UVA exposure. These specific effects were selected to evaluate the protection afforded by two sunscreens after topical application on the skin surface. Simplified formulations having different absorption spectra but similar sun protection factors were used. One contained a classical UVB absorber, 2-ethylhexyl-p-methoxycinnamate. The other contained a broad-spectrum absorber called Mexoryl SX, characterized by its strong absorbing potency in the UVA range. Both filters were used at 5% in a simple water/oil vehicle. The evaluation of photoprotection on in vitro reconstructed skin revealed good efficiency for both preparations in preventing UVB-induced damage, as shown by SBC counting and pyrimidine dimer immunostaining. By contrast, only the Mexoryl SX-containing preparation was able to efficiently prevent UVA-specific damage such as dermal fibroblast disappearance. Our data further support the fact that skin reconstructed in vitro is a reliable system to evaluate the photoprotection provided by different sunscreens against specific UVB and UVA biological damage. SN - 0031-8655 UR - https://www.unboundmedicine.com/medline/citation/10732449/Evaluation_of_the_protective_effect_of_sunscreens_on_in_vitro_reconstructed_human_skin_exposed_to_UVB_or_UVA_irradiation_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0031-8655&date=2000&volume=71&issue=3&spage=314 DB - PRIME DP - Unbound Medicine ER -