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Expression of telomerase-associated protein 1 and telomerase reverse transcriptase in hepatocellular carcinoma.
Br J Cancer. 2000 Feb; 82(4):833-7.BJ

Abstract

To know whether two protein components of human telomerase (human telomerase-associated protein 1 (hTEP1) and human telomerase reverse transcriptase (hTERT) are useful markers for telomerase activation in human liver diseases, we examined mRNA levels of these and telomerase activity in human liver samples. Twenty-three human hepatocellular carcinomas (HCCs) and corresponding adjacent livers were analysed for hTEP1 and hTERT expression by semiquantitative reverse transcription-polymerase chain reaction, and for telomerase activity by a telomeric repeat amplification protocol assay. Thirteen liver samples (ten HCCs and three dysplastic nodules) that were biopsied with 21-gauge needles were analysed for hTERT expression. hTEP1 was expressed in all samples examined. No correlation between hTEP1 expression and telomerase activity was observed. hTERT expression significantly correlated with telomerase activity (P< 0.001). The positivity of hTERT for HCC and corresponding non-cancerous liver was 100% and 30.4% respectively (P < 0.001). Seventy-four per cent (17/23) of HCCs showed strong hTERT expression, but none of the non-cancerous liver tissues did. hTERT expression of the 21-gauge needle biopsied specimens showed no significant difference from that of the surgical samples. The present study revealed that hTERT is strongly expressed in most HCCs, and that hTERT but not hTEP1 is a key component regulating telomerase activity in human liver.

Authors+Show Affiliations

First Department of Internal Medicine, Okayama University Medical School, Okayama-city, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10732755

Citation

Toshikuni, N, et al. "Expression of Telomerase-associated Protein 1 and Telomerase Reverse Transcriptase in Hepatocellular Carcinoma." British Journal of Cancer, vol. 82, no. 4, 2000, pp. 833-7.
Toshikuni N, Nouso K, Higashi T, et al. Expression of telomerase-associated protein 1 and telomerase reverse transcriptase in hepatocellular carcinoma. Br J Cancer. 2000;82(4):833-7.
Toshikuni, N., Nouso, K., Higashi, T., Nakatsukasa, H., Onishi, T., Kaneyoshi, T., Kobayashi, Y., Kariyama, K., Yamamoto, K., & Tsuji, T. (2000). Expression of telomerase-associated protein 1 and telomerase reverse transcriptase in hepatocellular carcinoma. British Journal of Cancer, 82(4), 833-7.
Toshikuni N, et al. Expression of Telomerase-associated Protein 1 and Telomerase Reverse Transcriptase in Hepatocellular Carcinoma. Br J Cancer. 2000;82(4):833-7. PubMed PMID: 10732755.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of telomerase-associated protein 1 and telomerase reverse transcriptase in hepatocellular carcinoma. AU - Toshikuni,N, AU - Nouso,K, AU - Higashi,T, AU - Nakatsukasa,H, AU - Onishi,T, AU - Kaneyoshi,T, AU - Kobayashi,Y, AU - Kariyama,K, AU - Yamamoto,K, AU - Tsuji,T, PY - 2000/3/25/pubmed PY - 2000/3/25/medline PY - 2000/3/25/entrez SP - 833 EP - 7 JF - British journal of cancer JO - Br J Cancer VL - 82 IS - 4 N2 - To know whether two protein components of human telomerase (human telomerase-associated protein 1 (hTEP1) and human telomerase reverse transcriptase (hTERT) are useful markers for telomerase activation in human liver diseases, we examined mRNA levels of these and telomerase activity in human liver samples. Twenty-three human hepatocellular carcinomas (HCCs) and corresponding adjacent livers were analysed for hTEP1 and hTERT expression by semiquantitative reverse transcription-polymerase chain reaction, and for telomerase activity by a telomeric repeat amplification protocol assay. Thirteen liver samples (ten HCCs and three dysplastic nodules) that were biopsied with 21-gauge needles were analysed for hTERT expression. hTEP1 was expressed in all samples examined. No correlation between hTEP1 expression and telomerase activity was observed. hTERT expression significantly correlated with telomerase activity (P< 0.001). The positivity of hTERT for HCC and corresponding non-cancerous liver was 100% and 30.4% respectively (P < 0.001). Seventy-four per cent (17/23) of HCCs showed strong hTERT expression, but none of the non-cancerous liver tissues did. hTERT expression of the 21-gauge needle biopsied specimens showed no significant difference from that of the surgical samples. The present study revealed that hTERT is strongly expressed in most HCCs, and that hTERT but not hTEP1 is a key component regulating telomerase activity in human liver. SN - 0007-0920 UR - https://www.unboundmedicine.com/medline/citation/10732755/Expression_of_telomerase_associated_protein_1_and_telomerase_reverse_transcriptase_in_hepatocellular_carcinoma_ L2 - https://doi.org/10.1054/bjoc.1999.1008 DB - PRIME DP - Unbound Medicine ER -