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Reactive oxygen species requirements for bovine sperm capacitation and acrosome reaction.
Theriogenology 1999; 52(2):289-301T

Abstract

Sperm capacitation is necessary for the fertilization of oocytes. During capacitation intracellular and membrane changes occur, that culminate with an exocytotic event called the acrosome reaction. The aim of this work was to study the participation of the superoxide anion (O2-.) and of hydrogen peroxide (H2O2) in the capacitation process and acrosome reaction in spermatozoa from cryopreserved bovine semen. Samples were capacitated with heparin or treated with the xanthine-xanthine oxidase-catalase system (X-XO-C) for the production of O2-. The percentage of capacitated spermatozoa was determined using the chlortetracycline (CTC) technique, by means of epifluorescence microscopy. Addition of X-XO-C to the incubation medium significantly induced capacitation (P < 0.05), but there were no differences with samples incubated with heparin. When the medium contained heparin or the X-XO-C, addition of superoxide dismutase (SOD, 0.5 mg/mL) significantly inhibited capacitation (P < 0.05). In samples treated with heparin and with diverse concentrations of H2O2 (10, 25, 50 and 250 microM) in the incubation medium, the percentage of capacitated spermatozoa was significantly reduced (P < 0.05); however, acrosome reaction was produced at concentrations of 10 and 25 microM H2O2. At concentrations greater than 25 microM H2O2 a deleterious effect was observed on sperm motility. From these results it may be inferred that O2-. is required in the capacitation process and that H2O2 may participate as an inductor of the acrosome reaction in spermatozoa from cryopreserved bovine semen.

Authors+Show Affiliations

School of Veterinary Sciences, University of Buenos Aires, Argentina.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10734395

Citation

O'Flaherty, C M., et al. "Reactive Oxygen Species Requirements for Bovine Sperm Capacitation and Acrosome Reaction." Theriogenology, vol. 52, no. 2, 1999, pp. 289-301.
O'Flaherty CM, Beorlegui NB, Beconi MT. Reactive oxygen species requirements for bovine sperm capacitation and acrosome reaction. Theriogenology. 1999;52(2):289-301.
O'Flaherty, C. M., Beorlegui, N. B., & Beconi, M. T. (1999). Reactive oxygen species requirements for bovine sperm capacitation and acrosome reaction. Theriogenology, 52(2), pp. 289-301.
O'Flaherty CM, Beorlegui NB, Beconi MT. Reactive Oxygen Species Requirements for Bovine Sperm Capacitation and Acrosome Reaction. Theriogenology. 1999 Jul 15;52(2):289-301. PubMed PMID: 10734395.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reactive oxygen species requirements for bovine sperm capacitation and acrosome reaction. AU - O'Flaherty,C M, AU - Beorlegui,N B, AU - Beconi,M T, PY - 2000/3/29/pubmed PY - 2000/4/25/medline PY - 2000/3/29/entrez SP - 289 EP - 301 JF - Theriogenology JO - Theriogenology VL - 52 IS - 2 N2 - Sperm capacitation is necessary for the fertilization of oocytes. During capacitation intracellular and membrane changes occur, that culminate with an exocytotic event called the acrosome reaction. The aim of this work was to study the participation of the superoxide anion (O2-.) and of hydrogen peroxide (H2O2) in the capacitation process and acrosome reaction in spermatozoa from cryopreserved bovine semen. Samples were capacitated with heparin or treated with the xanthine-xanthine oxidase-catalase system (X-XO-C) for the production of O2-. The percentage of capacitated spermatozoa was determined using the chlortetracycline (CTC) technique, by means of epifluorescence microscopy. Addition of X-XO-C to the incubation medium significantly induced capacitation (P < 0.05), but there were no differences with samples incubated with heparin. When the medium contained heparin or the X-XO-C, addition of superoxide dismutase (SOD, 0.5 mg/mL) significantly inhibited capacitation (P < 0.05). In samples treated with heparin and with diverse concentrations of H2O2 (10, 25, 50 and 250 microM) in the incubation medium, the percentage of capacitated spermatozoa was significantly reduced (P < 0.05); however, acrosome reaction was produced at concentrations of 10 and 25 microM H2O2. At concentrations greater than 25 microM H2O2 a deleterious effect was observed on sperm motility. From these results it may be inferred that O2-. is required in the capacitation process and that H2O2 may participate as an inductor of the acrosome reaction in spermatozoa from cryopreserved bovine semen. SN - 0093-691X UR - https://www.unboundmedicine.com/medline/citation/10734395/Reactive_oxygen_species_requirements_for_bovine_sperm_capacitation_and_acrosome_reaction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0093-691X(99)00129-6 DB - PRIME DP - Unbound Medicine ER -