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Optimization of chromatographic parameters for the determination of biogenic amines in wines by reversed-phase high-performance liquid chromatography.
J Chromatogr A. 2000 Feb 25; 871(1-2):75-83.JC

Abstract

A method suitable for the determination of eight biogenic amines (histamine, tyramine, phenylethylamine, tryptamine, cadaverine, putrescine, spermidine and spermine) in wines has been developed. The method involves derivatization of the amines by treatment with dabsyl chloride, after which the derivates were analysed by reversed-phase liquid chromatography with gradient elution and spectrophotometric detection at 446 nm. Different variables affecting separation were optimized. Validation of the method included calibration experiments, the addition of standards amines for the determination of recovery and repeatability tests. Good linearity of the responses was obtained up to 500 microg l(-1), except for putrescine (up to 2100 microg l(-1)). The detection limits ranged between 10 and 60 microg l(-1) for standard solutions. The method was successfully applied to the analysis of five Spanish wines.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10735288

Citation

Romero, R, et al. "Optimization of Chromatographic Parameters for the Determination of Biogenic Amines in Wines By Reversed-phase High-performance Liquid Chromatography." Journal of Chromatography. A, vol. 871, no. 1-2, 2000, pp. 75-83.
Romero R, Gázquez D, Bagur MG, et al. Optimization of chromatographic parameters for the determination of biogenic amines in wines by reversed-phase high-performance liquid chromatography. J Chromatogr A. 2000;871(1-2):75-83.
Romero, R., Gázquez, D., Bagur, M. G., & Sánchez-Viñas, M. (2000). Optimization of chromatographic parameters for the determination of biogenic amines in wines by reversed-phase high-performance liquid chromatography. Journal of Chromatography. A, 871(1-2), 75-83.
Romero R, et al. Optimization of Chromatographic Parameters for the Determination of Biogenic Amines in Wines By Reversed-phase High-performance Liquid Chromatography. J Chromatogr A. 2000 Feb 25;871(1-2):75-83. PubMed PMID: 10735288.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of chromatographic parameters for the determination of biogenic amines in wines by reversed-phase high-performance liquid chromatography. AU - Romero,R, AU - Gázquez,D, AU - Bagur,M G, AU - Sánchez-Viñas,M, PY - 2000/3/29/pubmed PY - 2000/6/3/medline PY - 2000/3/29/entrez SP - 75 EP - 83 JF - Journal of chromatography. A JO - J Chromatogr A VL - 871 IS - 1-2 N2 - A method suitable for the determination of eight biogenic amines (histamine, tyramine, phenylethylamine, tryptamine, cadaverine, putrescine, spermidine and spermine) in wines has been developed. The method involves derivatization of the amines by treatment with dabsyl chloride, after which the derivates were analysed by reversed-phase liquid chromatography with gradient elution and spectrophotometric detection at 446 nm. Different variables affecting separation were optimized. Validation of the method included calibration experiments, the addition of standards amines for the determination of recovery and repeatability tests. Good linearity of the responses was obtained up to 500 microg l(-1), except for putrescine (up to 2100 microg l(-1)). The detection limits ranged between 10 and 60 microg l(-1) for standard solutions. The method was successfully applied to the analysis of five Spanish wines. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/10735288/Optimization_of_chromatographic_parameters_for_the_determination_of_biogenic_amines_in_wines_by_reversed_phase_high_performance_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(99)00946-2 DB - PRIME DP - Unbound Medicine ER -