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Assessment of gastric carcinoma risk associated with Helicobacter pylori may vary depending on the antigen used: CagA specific enzyme-linked immunoadsorbent assay (ELISA) versus commercially available H. pylori ELISAs.
Cancer 2000; 88(7):1530-5C

Abstract

BACKGROUND

Previous epidemiologic studies produced inconsistent results when examining the relation between Helicobacter pylori infection and the risk of gastric carcinoma by measuring various anti-H. pylori antibodies. This study investigated the increased risk of cancer by examining different antibodies, including the specific anti-CagA antibody and antibodies from two commercially available kits.

METHODS

An ELISA for the detection of serum anti-CagA was established using a recombinant CagA protein that the authors previously reported. Serum anti-CagA titer was determined for 80 patients with gastric carcinoma and 80 gender- and age-matched controls. Two anti-H. pylori antibodies from the commercially available kits HEL-p (Amrad, Kew Vic, Australia) and HM-CAP (Enteric Product Inc., Westbury, NY) were also evaluated.

RESULTS

Anti-CagA seropositivity differed significantly between gastric carcinoma patients and controls (92.5% vs. 55.0%; P = 0. 0001), showing an odds ratio of 10.4 (95% confidence interval [CI]: 4.23-29.74). The difference was less prominent for the seropositivity of HEL-p (77.5% vs. 58.8%; P = 0.0139; odds ratio: 2. 38; 95% CI: 1.20-4.82) and insignificant for that of HM-CAP (65.0% vs. 57.5%; P = 0.4325; odds ratio: 1.30; 95% CI: 0.68-2.49).

CONCLUSIONS

The current study revealed that the antibody assay system used could be one important factor in the assessment of gastric carcinoma risk for patients with H. pylori.

Authors+Show Affiliations

Department of Gastroenterology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

10738209

Citation

Maeda, S, et al. "Assessment of Gastric Carcinoma Risk Associated With Helicobacter Pylori May Vary Depending On the Antigen Used: CagA Specific Enzyme-linked Immunoadsorbent Assay (ELISA) Versus Commercially Available H. Pylori ELISAs." Cancer, vol. 88, no. 7, 2000, pp. 1530-5.
Maeda S, Yoshida H, Ogura K, et al. Assessment of gastric carcinoma risk associated with Helicobacter pylori may vary depending on the antigen used: CagA specific enzyme-linked immunoadsorbent assay (ELISA) versus commercially available H. pylori ELISAs. Cancer. 2000;88(7):1530-5.
Maeda, S., Yoshida, H., Ogura, K., Yamaji, Y., Ikenoue, T., Mitsushima, T., ... Omata, M. (2000). Assessment of gastric carcinoma risk associated with Helicobacter pylori may vary depending on the antigen used: CagA specific enzyme-linked immunoadsorbent assay (ELISA) versus commercially available H. pylori ELISAs. Cancer, 88(7), pp. 1530-5.
Maeda S, et al. Assessment of Gastric Carcinoma Risk Associated With Helicobacter Pylori May Vary Depending On the Antigen Used: CagA Specific Enzyme-linked Immunoadsorbent Assay (ELISA) Versus Commercially Available H. Pylori ELISAs. Cancer. 2000 Apr 1;88(7):1530-5. PubMed PMID: 10738209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of gastric carcinoma risk associated with Helicobacter pylori may vary depending on the antigen used: CagA specific enzyme-linked immunoadsorbent assay (ELISA) versus commercially available H. pylori ELISAs. AU - Maeda,S, AU - Yoshida,H, AU - Ogura,K, AU - Yamaji,Y, AU - Ikenoue,T, AU - Mitsushima,T, AU - Tagawa,H, AU - Kawaguchi,R, AU - Mori,K, AU - Mafune,K i, AU - Kawabe,T, AU - Shiratori,Y, AU - Omata,M, PY - 2000/3/30/pubmed PY - 2000/4/29/medline PY - 2000/3/30/entrez SP - 1530 EP - 5 JF - Cancer JO - Cancer VL - 88 IS - 7 N2 - BACKGROUND: Previous epidemiologic studies produced inconsistent results when examining the relation between Helicobacter pylori infection and the risk of gastric carcinoma by measuring various anti-H. pylori antibodies. This study investigated the increased risk of cancer by examining different antibodies, including the specific anti-CagA antibody and antibodies from two commercially available kits. METHODS: An ELISA for the detection of serum anti-CagA was established using a recombinant CagA protein that the authors previously reported. Serum anti-CagA titer was determined for 80 patients with gastric carcinoma and 80 gender- and age-matched controls. Two anti-H. pylori antibodies from the commercially available kits HEL-p (Amrad, Kew Vic, Australia) and HM-CAP (Enteric Product Inc., Westbury, NY) were also evaluated. RESULTS: Anti-CagA seropositivity differed significantly between gastric carcinoma patients and controls (92.5% vs. 55.0%; P = 0. 0001), showing an odds ratio of 10.4 (95% confidence interval [CI]: 4.23-29.74). The difference was less prominent for the seropositivity of HEL-p (77.5% vs. 58.8%; P = 0.0139; odds ratio: 2. 38; 95% CI: 1.20-4.82) and insignificant for that of HM-CAP (65.0% vs. 57.5%; P = 0.4325; odds ratio: 1.30; 95% CI: 0.68-2.49). CONCLUSIONS: The current study revealed that the antibody assay system used could be one important factor in the assessment of gastric carcinoma risk for patients with H. pylori. SN - 0008-543X UR - https://www.unboundmedicine.com/medline/citation/10738209/Assessment_of_gastric_carcinoma_risk_associated_with_Helicobacter_pylori_may_vary_depending_on_the_antigen_used:_CagA_specific_enzyme_linked_immunoadsorbent_assay__ELISA__versus_commercially_available_H__pylori_ELISAs_ L2 - https://medlineplus.gov/helicobacterpyloriinfections.html DB - PRIME DP - Unbound Medicine ER -