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Exclusion of deep venous thrombosis with D-dimer testing--comparison of 13 D-dimer methods in 99 outpatients suspected of deep venous thrombosis using venography as reference standard.
Thromb Haemost. 2000 Feb; 83(2):191-8.TH

Abstract

In a direct assay comparison we evaluated the diagnostic performance of 10 novel D-Dimer assays for the exclusion of deep venous thrombosis (DVT). In addition, 3 conventional ELISA D-Dimer assays were included as reference tests. The study was performed in 99 consecutive outpatients referred to the emergency department for clinical suspicion of DVT. Venography was used as reference standard and demonstrated the presence of DVT in 50 patients (6 patients with isolated distal DVT and 44 patients with proximal DVT). The qualitative D-Dimer assays Minutex and SimpliRED and the quantitative BC DD showed overall sensitivities (for proximal and distal DVT) of only 80-83% with specificities that ranged from 87 to 94%. Overall sensitivity was 94% for the qualitative INSTANT I.A. and 98% for the quantitative Turbiquant at a cut-off level equal to the detection limit. Using different cut-off levels a sensitivity of 100% for proximal DVT and for proximal as well as distal DVT could be obtained for NycoCard, IL DD, Liatest, Tinaquant and VIDAS D-Dimer assays with specificities that ranged from 31% (NycoCard) to 71% (VIDAS) for proximal DVT and from 12% (NycoCard) to 47% (IL DD) for overall DVT. At a cut-off level equal to the upper limit of the reference range only Tinaquant and VIDAS showed a sensitivity of 100% for proximal as well as for distal DVT with a specificity of 39% and 41% respectively. The results of this study suggest that the VIDAS and Tinaquant D-Dimer assays have the highest sensitivity for the exclusion of DVT in outpatients. In outpatients that have a low or moderate pretest probability for DVT, these tests may be used in management studies where anticoagulation is withheld on the basis of D-Dimer testing alone.

Authors+Show Affiliations

Clinical Laboratories, Saint Joseph Hospital, Veldhoven, The Netherlands. fvdgraaf@sjz.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10739371

Citation

van der Graaf, F, et al. "Exclusion of Deep Venous Thrombosis With D-dimer Testing--comparison of 13 D-dimer Methods in 99 Outpatients Suspected of Deep Venous Thrombosis Using Venography as Reference Standard." Thrombosis and Haemostasis, vol. 83, no. 2, 2000, pp. 191-8.
van der Graaf F, van den Borne H, van der Kolk M, et al. Exclusion of deep venous thrombosis with D-dimer testing--comparison of 13 D-dimer methods in 99 outpatients suspected of deep venous thrombosis using venography as reference standard. Thromb Haemost. 2000;83(2):191-8.
van der Graaf, F., van den Borne, H., van der Kolk, M., de Wild, P. J., Janssen, G. W., & van Uum, S. H. (2000). Exclusion of deep venous thrombosis with D-dimer testing--comparison of 13 D-dimer methods in 99 outpatients suspected of deep venous thrombosis using venography as reference standard. Thrombosis and Haemostasis, 83(2), 191-8.
van der Graaf F, et al. Exclusion of Deep Venous Thrombosis With D-dimer Testing--comparison of 13 D-dimer Methods in 99 Outpatients Suspected of Deep Venous Thrombosis Using Venography as Reference Standard. Thromb Haemost. 2000;83(2):191-8. PubMed PMID: 10739371.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exclusion of deep venous thrombosis with D-dimer testing--comparison of 13 D-dimer methods in 99 outpatients suspected of deep venous thrombosis using venography as reference standard. AU - van der Graaf,F, AU - van den Borne,H, AU - van der Kolk,M, AU - de Wild,P J, AU - Janssen,G W, AU - van Uum,S H, PY - 2000/3/30/pubmed PY - 2000/5/16/medline PY - 2000/3/30/entrez SP - 191 EP - 8 JF - Thrombosis and haemostasis JO - Thromb. Haemost. VL - 83 IS - 2 N2 - In a direct assay comparison we evaluated the diagnostic performance of 10 novel D-Dimer assays for the exclusion of deep venous thrombosis (DVT). In addition, 3 conventional ELISA D-Dimer assays were included as reference tests. The study was performed in 99 consecutive outpatients referred to the emergency department for clinical suspicion of DVT. Venography was used as reference standard and demonstrated the presence of DVT in 50 patients (6 patients with isolated distal DVT and 44 patients with proximal DVT). The qualitative D-Dimer assays Minutex and SimpliRED and the quantitative BC DD showed overall sensitivities (for proximal and distal DVT) of only 80-83% with specificities that ranged from 87 to 94%. Overall sensitivity was 94% for the qualitative INSTANT I.A. and 98% for the quantitative Turbiquant at a cut-off level equal to the detection limit. Using different cut-off levels a sensitivity of 100% for proximal DVT and for proximal as well as distal DVT could be obtained for NycoCard, IL DD, Liatest, Tinaquant and VIDAS D-Dimer assays with specificities that ranged from 31% (NycoCard) to 71% (VIDAS) for proximal DVT and from 12% (NycoCard) to 47% (IL DD) for overall DVT. At a cut-off level equal to the upper limit of the reference range only Tinaquant and VIDAS showed a sensitivity of 100% for proximal as well as for distal DVT with a specificity of 39% and 41% respectively. The results of this study suggest that the VIDAS and Tinaquant D-Dimer assays have the highest sensitivity for the exclusion of DVT in outpatients. In outpatients that have a low or moderate pretest probability for DVT, these tests may be used in management studies where anticoagulation is withheld on the basis of D-Dimer testing alone. SN - 0340-6245 UR - https://www.unboundmedicine.com/medline/citation/10739371/Exclusion_of_deep_venous_thrombosis_with_D_dimer_testing__comparison_of_13_D_dimer_methods_in_99_outpatients_suspected_of_deep_venous_thrombosis_using_venography_as_reference_standard_ L2 - http://www.diseaseinfosearch.org/result/7087 DB - PRIME DP - Unbound Medicine ER -