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Crystal structure of the S15-rRNA complex.
Nat Struct Biol. 2000 Apr; 7(4):273-7.NS

Abstract

In bacterial ribosomes, the small (30S) ribosomal subunit is composed of 16S rRNA and 21 distinct proteins. Ribosomal protein S15 is of particular interest because it binds primarily to 16S rRNA and is required for assembly of the small subunit and for intersubunit association, thus representing a key element in the assembly of a whole ribosome. Here we report the 2.8 ¿ resolution crystal structure of the highly conserved S15-rRNA complex. Protein S15 interacts in the minor groove with a G-U/G-C motif and a three-way junction. The latter is constrained by a conserved base triple and stacking interactions, and locked into place by magnesium ions and protein side chains, mainly through interactions with the unique three-dimensional geometry of the backbone. The present structure gives insights into the dual role of S15 in ribosome assembly and translational regulation.

Authors+Show Affiliations

Nikulin A
Institute of Protein Research, Pushchino, Moscow Region, 142292, Russia.
Serganov A
No affiliation info available
Ennifar E
No affiliation info available
Tishchenko S
No affiliation info available
Nevskaya N
No affiliation info available
Shepard W
No affiliation info available
Portier C
No affiliation info available
Garber M
No affiliation info available
Ehresmann B
No affiliation info available
Ehresmann C
No affiliation info available
Nikonov S
No affiliation info available
Dumas P
No affiliation info available

MeSH

Amino Acid SequenceBase PairingBase SequenceBinding SitesConserved SequenceCrystallography, X-RayMagnesiumModels, MolecularMolecular Sequence DataNucleic Acid ConformationProtein ConformationRNA, BacterialRNA, Ribosomal, 16SRibosomal ProteinsStructure-Activity RelationshipThermus thermophilus

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10742169

Citation

Nikulin, A, et al. "Crystal Structure of the S15-rRNA Complex." Nature Structural Biology, vol. 7, no. 4, 2000, pp. 273-7.
Nikulin A, Serganov A, Ennifar E, et al. Crystal structure of the S15-rRNA complex. Nat Struct Biol. 2000;7(4):273-7.
Nikulin, A., Serganov, A., Ennifar, E., Tishchenko, S., Nevskaya, N., Shepard, W., Portier, C., Garber, M., Ehresmann, B., Ehresmann, C., Nikonov, S., & Dumas, P. (2000). Crystal structure of the S15-rRNA complex. Nature Structural Biology, 7(4), 273-7.
Nikulin A, et al. Crystal Structure of the S15-rRNA Complex. Nat Struct Biol. 2000;7(4):273-7. PubMed PMID: 10742169.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crystal structure of the S15-rRNA complex. AU - Nikulin,A, AU - Serganov,A, AU - Ennifar,E, AU - Tishchenko,S, AU - Nevskaya,N, AU - Shepard,W, AU - Portier,C, AU - Garber,M, AU - Ehresmann,B, AU - Ehresmann,C, AU - Nikonov,S, AU - Dumas,P, PY - 2000/3/31/pubmed PY - 2000/5/8/medline PY - 2000/3/31/entrez SP - 273 EP - 7 JF - Nature structural biology JO - Nat Struct Biol VL - 7 IS - 4 N2 - In bacterial ribosomes, the small (30S) ribosomal subunit is composed of 16S rRNA and 21 distinct proteins. Ribosomal protein S15 is of particular interest because it binds primarily to 16S rRNA and is required for assembly of the small subunit and for intersubunit association, thus representing a key element in the assembly of a whole ribosome. Here we report the 2.8 ¿ resolution crystal structure of the highly conserved S15-rRNA complex. Protein S15 interacts in the minor groove with a G-U/G-C motif and a three-way junction. The latter is constrained by a conserved base triple and stacking interactions, and locked into place by magnesium ions and protein side chains, mainly through interactions with the unique three-dimensional geometry of the backbone. The present structure gives insights into the dual role of S15 in ribosome assembly and translational regulation. SN - 1072-8368 UR - https://www.unboundmedicine.com/medline/citation/10742169/Crystal_structure_of_the_S15_rRNA_complex_ L2 - https://biocyc.org/gene?orgid=PPUT160488&id=G1G01-5031 DB - PRIME DP - Unbound Medicine ER -