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Extracellular polysaccharide of Erwinia chrysanthemi A350 and ribotyping of Erwinia chrysanthemi spp.
Carbohydr Res. 2000 Mar 10; 324(4):255-67.CR

Abstract

Erwinia chrysanthemi spp. are gram-negative bacterial phytopathogens causing soft rots in a number of plants. The structure of the extracellular polysaccharide (EPS) produced by the E. chrysanthemi strain A350, which is a lacZ- mutant of the wild type strain 3937, pathogenic to Saintpaulia, has been determined using a combination of chemical and physical techniques including methylation analysis, low-pressure gel-filtration and anion-exchange chromatography, high-pH anion-exchange chromatography, partial acid hydrolysis, mass spectrometry and 1- and 2D NMR spectroscopy. In contrast to the structures of the EPS reported for other strains of E. chrysanthemi, the EPS from strain A350 contains D-GalA, together with L-Rhap and D-Galp in a 1:4:1 ratio. Evidence is presented for the following hexasaccharide repeat unit: [structure: see text] All the Erwinia chrysanthemi spp. studied to date have been analyzed by ribotyping and collated into families, which are consistent with the related structures of their EPS.

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Authors+Show Affiliations

Gray JS
Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA.
Yang BY
No affiliation info available
Montgomery R
No affiliation info available

MeSH

Carbohydrate ConformationCarbohydrate SequenceChromatography, GelChromatography, High Pressure LiquidDickeya chrysanthemiMagnetic Resonance SpectroscopyMass SpectrometryMolecular Sequence DataMonosaccharidesOligosaccharidesPlant DiseasesPolysaccharides, BacterialSerology

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

10744334

Citation

Gray, J S., et al. "Extracellular Polysaccharide of Erwinia Chrysanthemi A350 and Ribotyping of Erwinia Chrysanthemi Spp." Carbohydrate Research, vol. 324, no. 4, 2000, pp. 255-67.
Gray JS, Yang BY, Montgomery R. Extracellular polysaccharide of Erwinia chrysanthemi A350 and ribotyping of Erwinia chrysanthemi spp. Carbohydr Res. 2000;324(4):255-67.
Gray, J. S., Yang, B. Y., & Montgomery, R. (2000). Extracellular polysaccharide of Erwinia chrysanthemi A350 and ribotyping of Erwinia chrysanthemi spp. Carbohydrate Research, 324(4), 255-67.
Gray JS, Yang BY, Montgomery R. Extracellular Polysaccharide of Erwinia Chrysanthemi A350 and Ribotyping of Erwinia Chrysanthemi Spp. Carbohydr Res. 2000 Mar 10;324(4):255-67. PubMed PMID: 10744334.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extracellular polysaccharide of Erwinia chrysanthemi A350 and ribotyping of Erwinia chrysanthemi spp. AU - Gray,J S, AU - Yang,B Y, AU - Montgomery,R, PY - 2000/4/1/pubmed PY - 2000/6/8/medline PY - 2000/4/1/entrez SP - 255 EP - 67 JF - Carbohydrate research JO - Carbohydr Res VL - 324 IS - 4 N2 - Erwinia chrysanthemi spp. are gram-negative bacterial phytopathogens causing soft rots in a number of plants. The structure of the extracellular polysaccharide (EPS) produced by the E. chrysanthemi strain A350, which is a lacZ- mutant of the wild type strain 3937, pathogenic to Saintpaulia, has been determined using a combination of chemical and physical techniques including methylation analysis, low-pressure gel-filtration and anion-exchange chromatography, high-pH anion-exchange chromatography, partial acid hydrolysis, mass spectrometry and 1- and 2D NMR spectroscopy. In contrast to the structures of the EPS reported for other strains of E. chrysanthemi, the EPS from strain A350 contains D-GalA, together with L-Rhap and D-Galp in a 1:4:1 ratio. Evidence is presented for the following hexasaccharide repeat unit: [structure: see text] All the Erwinia chrysanthemi spp. studied to date have been analyzed by ribotyping and collated into families, which are consistent with the related structures of their EPS. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/10744334/Extracellular_polysaccharide_of_Erwinia_chrysanthemi_A350_and_ribotyping_of_Erwinia_chrysanthemi_spp_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0008621599002438 DB - PRIME DP - Unbound Medicine ER -