Tags

Type your tag names separated by a space and hit enter

[Serological diagnosis of celiac disease: anti-gliadin peptide antibodies and tissue anti-transglutaminase].
Medicina (B Aires) 1999; 59(6):693-7M

Abstract

Serological markers currently used for the diagnosis of celiac disease are anti-gliadin (AG) and anti-endomysium (AE) antibodies. Recently tissue transglutaminase (tTG) was identified as the specific autoantigen for endomysial antibodies. The aim of this work was to determine sensitivity and specificity of ELISA tests developed by using defined molecular structures as capture antigen for AG and AE antibodies. Three synthetic peptides, from the amino terminal region of alpha gliadin, were used as immobilized antigens for AG, and the transglutaminase from guinea pig liver for AE. A total of 80 sera from celiac patients, non celiac disease controls and healthy controls were examined. Age range was 7 months to 14 years. A sensitivity of 97% and a specificity of 86% was obtained for IgG determined by using as antigen one of the three synthetic peptides (corresponding to residues 31-55 of alpha gliadin). Therefore, this peptide appears as a highly sensitive antigen and more specific than gliadin. The best result, showing 100% of sensitivity and specificity, was obtained for IgA anti-tTG, thus pointing out the relevance of these antibodies as serological markers for celiac disease.

Authors+Show Affiliations

Cátedra de Bioquímica Básica, INTEBIO, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

10752210

Citation

Piaggio, M V., et al. "[Serological Diagnosis of Celiac Disease: Anti-gliadin Peptide Antibodies and Tissue Anti-transglutaminase]." Medicina, vol. 59, no. 6, 1999, pp. 693-7.
Piaggio MV, Demonte AM, Sihufe G, et al. [Serological diagnosis of celiac disease: anti-gliadin peptide antibodies and tissue anti-transglutaminase]. Medicina (B Aires). 1999;59(6):693-7.
Piaggio, M. V., Demonte, A. M., Sihufe, G., Garcilazo, S., Esper, M. C., Wagener, M., & Aleanzi, M. (1999). [Serological diagnosis of celiac disease: anti-gliadin peptide antibodies and tissue anti-transglutaminase]. Medicina, 59(6), pp. 693-7.
Piaggio MV, et al. [Serological Diagnosis of Celiac Disease: Anti-gliadin Peptide Antibodies and Tissue Anti-transglutaminase]. Medicina (B Aires). 1999;59(6):693-7. PubMed PMID: 10752210.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Serological diagnosis of celiac disease: anti-gliadin peptide antibodies and tissue anti-transglutaminase]. AU - Piaggio,M V, AU - Demonte,A M, AU - Sihufe,G, AU - Garcilazo,S, AU - Esper,M C, AU - Wagener,M, AU - Aleanzi,M, PY - 2001/2/7/pubmed PY - 2001/2/7/medline PY - 2001/2/7/entrez SP - 693 EP - 7 JF - Medicina JO - Medicina (B Aires) VL - 59 IS - 6 N2 - Serological markers currently used for the diagnosis of celiac disease are anti-gliadin (AG) and anti-endomysium (AE) antibodies. Recently tissue transglutaminase (tTG) was identified as the specific autoantigen for endomysial antibodies. The aim of this work was to determine sensitivity and specificity of ELISA tests developed by using defined molecular structures as capture antigen for AG and AE antibodies. Three synthetic peptides, from the amino terminal region of alpha gliadin, were used as immobilized antigens for AG, and the transglutaminase from guinea pig liver for AE. A total of 80 sera from celiac patients, non celiac disease controls and healthy controls were examined. Age range was 7 months to 14 years. A sensitivity of 97% and a specificity of 86% was obtained for IgG determined by using as antigen one of the three synthetic peptides (corresponding to residues 31-55 of alpha gliadin). Therefore, this peptide appears as a highly sensitive antigen and more specific than gliadin. The best result, showing 100% of sensitivity and specificity, was obtained for IgA anti-tTG, thus pointing out the relevance of these antibodies as serological markers for celiac disease. SN - 0025-7680 UR - https://www.unboundmedicine.com/medline/citation/10752210/[Serological_diagnosis_of_celiac_disease:_anti_gliadin_peptide_antibodies_and_tissue_anti_transglutaminase]_ L2 - http://www.diseaseinfosearch.org/result/1186 DB - PRIME DP - Unbound Medicine ER -