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A foodborne outbreak of gastroenteritis associated with Norwalk-like viruses: first molecular traceback to deli sandwiches contaminated during preparation.
J Infect Dis. 2000 Apr; 181(4):1467-70.JI

Abstract

In March 1998, an outbreak of acute gastroenteritis occurred among students at a Texas university. Overall, 125 ill students sought medical care. Case-control studies revealed that illness was significantly associated with eating foods from the university's main cafeteria deli bar on 9 and 10 March. Stool specimens from 9 (50%) of 18 ill students and samples of deli ham showed evidence of Norwalk-like viruses (NLVs) by reverse-transcriptase (RT) polymerase chain reaction (PCR) assay. A food handler who prepared sandwiches for lunch on 9 March reported that her infant had been sick with watery diarrhea since just before the outbreak. A stool sample from the infant was positive for NLV by RT-PCR, and the sequence of the amplified product was identical to that of amplified product from deli ham and students' stool specimens. This is the first time RT-PCR and sequence analysis have successfully confirmed viral contamination of a food item likely to have been contaminated by a food handler.

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Authors+Show Affiliations

Daniels NA
Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, Atlanta, GA 30333, USA.
Bergmire-Sweat DA
No affiliation info available
Schwab KJ
No affiliation info available
Hendricks KA
No affiliation info available
Reddy S
No affiliation info available
Rowe SM
No affiliation info available
Fankhauser RL
No affiliation info available
Monroe SS
No affiliation info available
Atmar RL
No affiliation info available
Glass RI
No affiliation info available
Mead P
No affiliation info available

MeSH

AdolescentAdultCaliciviridae InfectionsCase-Control StudiesDiarrheaDisease OutbreaksFecesFemaleFood HandlingFoodborne DiseasesGastroenteritisHumansInfantMaleNorwalk virusPolymerase Chain ReactionTexasUniversities

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10753727

Citation

Daniels, N A., et al. "A Foodborne Outbreak of Gastroenteritis Associated With Norwalk-like Viruses: First Molecular Traceback to Deli Sandwiches Contaminated During Preparation." The Journal of Infectious Diseases, vol. 181, no. 4, 2000, pp. 1467-70.
Daniels NA, Bergmire-Sweat DA, Schwab KJ, et al. A foodborne outbreak of gastroenteritis associated with Norwalk-like viruses: first molecular traceback to deli sandwiches contaminated during preparation. J Infect Dis. 2000;181(4):1467-70.
Daniels, N. A., Bergmire-Sweat, D. A., Schwab, K. J., Hendricks, K. A., Reddy, S., Rowe, S. M., Fankhauser, R. L., Monroe, S. S., Atmar, R. L., Glass, R. I., & Mead, P. (2000). A foodborne outbreak of gastroenteritis associated with Norwalk-like viruses: first molecular traceback to deli sandwiches contaminated during preparation. The Journal of Infectious Diseases, 181(4), 1467-70.
Daniels NA, et al. A Foodborne Outbreak of Gastroenteritis Associated With Norwalk-like Viruses: First Molecular Traceback to Deli Sandwiches Contaminated During Preparation. J Infect Dis. 2000;181(4):1467-70. PubMed PMID: 10753727.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A foodborne outbreak of gastroenteritis associated with Norwalk-like viruses: first molecular traceback to deli sandwiches contaminated during preparation. AU - Daniels,N A, AU - Bergmire-Sweat,D A, AU - Schwab,K J, AU - Hendricks,K A, AU - Reddy,S, AU - Rowe,S M, AU - Fankhauser,R L, AU - Monroe,S S, AU - Atmar,R L, AU - Glass,R I, AU - Mead,P, Y1 - 2000/04/07/ PY - 1999/04/28/received PY - 1999/09/28/revised PY - 2000/4/8/pubmed PY - 2000/6/3/medline PY - 2000/4/8/entrez SP - 1467 EP - 70 JF - The Journal of infectious diseases JO - J Infect Dis VL - 181 IS - 4 N2 - In March 1998, an outbreak of acute gastroenteritis occurred among students at a Texas university. Overall, 125 ill students sought medical care. Case-control studies revealed that illness was significantly associated with eating foods from the university's main cafeteria deli bar on 9 and 10 March. Stool specimens from 9 (50%) of 18 ill students and samples of deli ham showed evidence of Norwalk-like viruses (NLVs) by reverse-transcriptase (RT) polymerase chain reaction (PCR) assay. A food handler who prepared sandwiches for lunch on 9 March reported that her infant had been sick with watery diarrhea since just before the outbreak. A stool sample from the infant was positive for NLV by RT-PCR, and the sequence of the amplified product was identical to that of amplified product from deli ham and students' stool specimens. This is the first time RT-PCR and sequence analysis have successfully confirmed viral contamination of a food item likely to have been contaminated by a food handler. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/10753727/A_foodborne_outbreak_of_gastroenteritis_associated_with_Norwalk_like_viruses:_first_molecular_traceback_to_deli_sandwiches_contaminated_during_preparation_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1086/315365 DB - PRIME DP - Unbound Medicine ER -