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Quorum sensing in the plant pathogen Erwinia carotovora subsp. carotovora: the role of expR(Ecc).
Mol Plant Microbe Interact. 2000 Apr; 13(4):384-93.MP

Abstract

The production of the main virulence determinants of the plant pathogen Erwinia carotovora subsp. carotovora, the extracellular cell wall-degrading enzymes, is partly controlled by the diffusible signal molecule N-(3-oxohexanoyl)-L-homoserine lactone (OHHL). OHHL is synthesized by the product of the expI/carI gene. Linked to expI we found a gene encoding a putative transcriptional regulator of the LuxR-family. This gene, expR(Ecc), is transcribed convergently to the expI gene and the two open reading frames are partially overlapping. The ExpR(Ecc) protein showed extensive amino acid sequence similarity to the repressor EsaR from Pantoea stewartii subsp. stewartii (formerly Erwinia stewartii subsp. stewartii) and to the ExpR(Ech) protein of Erwinia chrysanthemi. Inactivation of the E. carotovora subsp. carotovora expR(Ecc) gene caused no decrease in virulence or production of virulence determinants in vitro. In contrast, there was a slight increase in the maceration capacity of the mutant strain. The effects of ExpR(Ecc) were probably mediated by changes in OHHL levels. Inactivation of expR(Ecc) resulted in increased OHHL levels during early logarithmic growth. In addition, overexpression of expR(Ecc) caused a clear decrease in the production of virulence determinants and part of this effect was likely to be caused by OHHL binding to ExpR(Ecc). ExpR(Ecc) did not appear to exhibit transcriptional regulation of expI, but the effect on OHHL was apparently due to other mechanisms.

Authors+Show Affiliations

Department of Plant Biology, Uppsala Genetic Center, Swedish University of Agricultural Sciences.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10755301

Citation

Andersson, R A., et al. "Quorum Sensing in the Plant Pathogen Erwinia Carotovora Subsp. Carotovora: the Role of ExpR(Ecc)." Molecular Plant-microbe Interactions : MPMI, vol. 13, no. 4, 2000, pp. 384-93.
Andersson RA, Eriksson AR, Heikinheimo R, et al. Quorum sensing in the plant pathogen Erwinia carotovora subsp. carotovora: the role of expR(Ecc). Mol Plant Microbe Interact. 2000;13(4):384-93.
Andersson, R. A., Eriksson, A. R., Heikinheimo, R., Mäe, A., Pirhonen, M., Kõiv, V., Hyytiäinen, H., Tuikkala, A., & Palva, E. T. (2000). Quorum sensing in the plant pathogen Erwinia carotovora subsp. carotovora: the role of expR(Ecc). Molecular Plant-microbe Interactions : MPMI, 13(4), 384-93.
Andersson RA, et al. Quorum Sensing in the Plant Pathogen Erwinia Carotovora Subsp. Carotovora: the Role of ExpR(Ecc). Mol Plant Microbe Interact. 2000;13(4):384-93. PubMed PMID: 10755301.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quorum sensing in the plant pathogen Erwinia carotovora subsp. carotovora: the role of expR(Ecc). AU - Andersson,R A, AU - Eriksson,A R, AU - Heikinheimo,R, AU - Mäe,A, AU - Pirhonen,M, AU - Kõiv,V, AU - Hyytiäinen,H, AU - Tuikkala,A, AU - Palva,E T, PY - 2000/4/8/pubmed PY - 2000/7/6/medline PY - 2000/4/8/entrez SP - 384 EP - 93 JF - Molecular plant-microbe interactions : MPMI JO - Mol Plant Microbe Interact VL - 13 IS - 4 N2 - The production of the main virulence determinants of the plant pathogen Erwinia carotovora subsp. carotovora, the extracellular cell wall-degrading enzymes, is partly controlled by the diffusible signal molecule N-(3-oxohexanoyl)-L-homoserine lactone (OHHL). OHHL is synthesized by the product of the expI/carI gene. Linked to expI we found a gene encoding a putative transcriptional regulator of the LuxR-family. This gene, expR(Ecc), is transcribed convergently to the expI gene and the two open reading frames are partially overlapping. The ExpR(Ecc) protein showed extensive amino acid sequence similarity to the repressor EsaR from Pantoea stewartii subsp. stewartii (formerly Erwinia stewartii subsp. stewartii) and to the ExpR(Ech) protein of Erwinia chrysanthemi. Inactivation of the E. carotovora subsp. carotovora expR(Ecc) gene caused no decrease in virulence or production of virulence determinants in vitro. In contrast, there was a slight increase in the maceration capacity of the mutant strain. The effects of ExpR(Ecc) were probably mediated by changes in OHHL levels. Inactivation of expR(Ecc) resulted in increased OHHL levels during early logarithmic growth. In addition, overexpression of expR(Ecc) caused a clear decrease in the production of virulence determinants and part of this effect was likely to be caused by OHHL binding to ExpR(Ecc). ExpR(Ecc) did not appear to exhibit transcriptional regulation of expI, but the effect on OHHL was apparently due to other mechanisms. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/10755301/Quorum_sensing_in_the_plant_pathogen_Erwinia_carotovora_subsp__carotovora:_the_role_of_expR_Ecc__ L2 - https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.4.384?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -