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Protection from nitrosative stress by yeast flavohemoglobin.
Proc Natl Acad Sci U S A. 2000 Apr 25; 97(9):4672-6.PN

Abstract

Yeast hemoglobin was discovered close to half a century ago, but its function has remained unknown. Herein, we report that this flavohemoglobin protects Saccharomyces cerevisiae from nitrosative stress. Deletion of the flavohemoglobin gene (YHB1) abolished the nitric oxide (NO)-consuming activity of yeast cells. Levels of protein nitrosylation were more than 10-fold higher in yhb1 mutant yeast than in isogenic wild-type cells after incubation with NO donors. Growth of mutant cells was inhibited by a nitrosative challenge that had little effect on wild-type cells, whereas the resistance of mutant cells to oxidative stress was unimpaired. Protection conferred by yeast flavohemoglobin against NO and S-nitrosothiols was seen under both anaerobic and aerobic conditions, consistent with a primary function in NO detoxification. A phylogenetic analysis indicated that protection from nitrosative stress is likely to be a conserved function among microorganismal flavohemoglobins. Flavohemoglobin is therefore a potential target for antimicrobial therapy.

Authors+Show Affiliations

Howard Hughes Medical Institute, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10758168

Citation

Liu, L, et al. "Protection From Nitrosative Stress By Yeast Flavohemoglobin." Proceedings of the National Academy of Sciences of the United States of America, vol. 97, no. 9, 2000, pp. 4672-6.
Liu L, Zeng M, Hausladen A, et al. Protection from nitrosative stress by yeast flavohemoglobin. Proc Natl Acad Sci U S A. 2000;97(9):4672-6.
Liu, L., Zeng, M., Hausladen, A., Heitman, J., & Stamler, J. S. (2000). Protection from nitrosative stress by yeast flavohemoglobin. Proceedings of the National Academy of Sciences of the United States of America, 97(9), 4672-6.
Liu L, et al. Protection From Nitrosative Stress By Yeast Flavohemoglobin. Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4672-6. PubMed PMID: 10758168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protection from nitrosative stress by yeast flavohemoglobin. AU - Liu,L, AU - Zeng,M, AU - Hausladen,A, AU - Heitman,J, AU - Stamler,J S, PY - 2000/4/12/pubmed PY - 2000/6/8/medline PY - 2000/4/12/entrez SP - 4672 EP - 6 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 97 IS - 9 N2 - Yeast hemoglobin was discovered close to half a century ago, but its function has remained unknown. Herein, we report that this flavohemoglobin protects Saccharomyces cerevisiae from nitrosative stress. Deletion of the flavohemoglobin gene (YHB1) abolished the nitric oxide (NO)-consuming activity of yeast cells. Levels of protein nitrosylation were more than 10-fold higher in yhb1 mutant yeast than in isogenic wild-type cells after incubation with NO donors. Growth of mutant cells was inhibited by a nitrosative challenge that had little effect on wild-type cells, whereas the resistance of mutant cells to oxidative stress was unimpaired. Protection conferred by yeast flavohemoglobin against NO and S-nitrosothiols was seen under both anaerobic and aerobic conditions, consistent with a primary function in NO detoxification. A phylogenetic analysis indicated that protection from nitrosative stress is likely to be a conserved function among microorganismal flavohemoglobins. Flavohemoglobin is therefore a potential target for antimicrobial therapy. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/10758168/Protection_from_nitrosative_stress_by_yeast_flavohemoglobin_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=10758168 DB - PRIME DP - Unbound Medicine ER -