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Detection and identification of labeled DNA by surface enhanced resonance Raman scattering.
Biopolymers. 2000; 57(2):85-91.B

Abstract

The detection of specific sequences of DNA bases in a single strand can be achieved by hybridization of a known sequence of synthetic DNA. Due to the low concentrations usually used, a fluorescent label is required to detect the probe. Surface enhanced resonance Raman scattering (SERRS) also has the required sensitivity and provides a specific set of signals that are more applicable to discrimination of a number of probes without separation. A reliable SERRS method is reported here using two probes specifically designed for SERRS. It was possible to detect a 2 x 10(-12)M solution of labeled DNA, which illustrated the sensitive nature of SERRS for DNA analysis.

Authors+Show Affiliations

Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1XL, United Kingdom.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10766959

Citation

Graham, D, et al. "Detection and Identification of Labeled DNA By Surface Enhanced Resonance Raman Scattering." Biopolymers, vol. 57, no. 2, 2000, pp. 85-91.
Graham D, Mallinder BJ, Smith WE. Detection and identification of labeled DNA by surface enhanced resonance Raman scattering. Biopolymers. 2000;57(2):85-91.
Graham, D., Mallinder, B. J., & Smith, W. E. (2000). Detection and identification of labeled DNA by surface enhanced resonance Raman scattering. Biopolymers, 57(2), 85-91.
Graham D, Mallinder BJ, Smith WE. Detection and Identification of Labeled DNA By Surface Enhanced Resonance Raman Scattering. Biopolymers. 2000;57(2):85-91. PubMed PMID: 10766959.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection and identification of labeled DNA by surface enhanced resonance Raman scattering. AU - Graham,D, AU - Mallinder,B J, AU - Smith,W E, PY - 2000/4/15/pubmed PY - 2000/6/8/medline PY - 2000/4/15/entrez SP - 85 EP - 91 JF - Biopolymers JO - Biopolymers VL - 57 IS - 2 N2 - The detection of specific sequences of DNA bases in a single strand can be achieved by hybridization of a known sequence of synthetic DNA. Due to the low concentrations usually used, a fluorescent label is required to detect the probe. Surface enhanced resonance Raman scattering (SERRS) also has the required sensitivity and provides a specific set of signals that are more applicable to discrimination of a number of probes without separation. A reliable SERRS method is reported here using two probes specifically designed for SERRS. It was possible to detect a 2 x 10(-12)M solution of labeled DNA, which illustrated the sensitive nature of SERRS for DNA analysis. SN - 0006-3525 UR - https://www.unboundmedicine.com/medline/citation/10766959/Detection_and_identification_of_labeled_DNA_by_surface_enhanced_resonance_Raman_scattering_ L2 - https://doi.org/10.1002/(SICI)1097-0282(2000)57:2<85::AID-BIP5>3.0.CO;2-# DB - PRIME DP - Unbound Medicine ER -