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A new method of quantification of pipemidic-acid by capillary zone electrophoresis with end-column amperometric detection.
Electrophoresis. 2000 Mar; 21(5):925-9.E

Abstract

Capillary zone electrophoresis was employed for the determination of pipemidic acid using an end-column amperometric detection with a carbon fiber microdisk array electrode, at a constant potential of -1.10 V vs. saturated calomel electrode. The optimum conditions of separation and detection were 1.2 x 10(-4) mol/LNaOAc - 8.8 x 10(-4) mol/ LHOAc for the buffer solution, 20 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time. The limit of detection was 1.05 x 10(-7) mol/L or 189 amol (S/N=3). The relative standard deviation was 0.31% for the migration time and 2.0% for the electrophoretic peak current. The method was applied to determining pipemidic acid in human serum.

Authors+Show Affiliations

Jin W
Laboratory of Analytical Science, School of Chemistry, Shandong University, Jinan, China. wenrujin@jn-public.sd.cninfo.net
Yu D
No affiliation info available
Dong Q
No affiliation info available
Ye X
No affiliation info available

MeSH

Anti-Infective AgentsBuffersCarbonCarbon FiberElectrodesElectrophoresis, CapillaryHumansIndicators and ReagentsMercury CompoundsPipemidic AcidQuality ControlReproducibility of Results

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10768778

Citation

Jin, W, et al. "A New Method of Quantification of Pipemidic-acid By Capillary Zone Electrophoresis With End-column Amperometric Detection." Electrophoresis, vol. 21, no. 5, 2000, pp. 925-9.
Jin W, Yu D, Dong Q, et al. A new method of quantification of pipemidic-acid by capillary zone electrophoresis with end-column amperometric detection. Electrophoresis. 2000;21(5):925-9.
Jin, W., Yu, D., Dong, Q., & Ye, X. (2000). A new method of quantification of pipemidic-acid by capillary zone electrophoresis with end-column amperometric detection. Electrophoresis, 21(5), 925-9.
Jin W, et al. A New Method of Quantification of Pipemidic-acid By Capillary Zone Electrophoresis With End-column Amperometric Detection. Electrophoresis. 2000;21(5):925-9. PubMed PMID: 10768778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new method of quantification of pipemidic-acid by capillary zone electrophoresis with end-column amperometric detection. AU - Jin,W, AU - Yu,D, AU - Dong,Q, AU - Ye,X, PY - 2000/4/18/pubmed PY - 2000/6/10/medline PY - 2000/4/18/entrez SP - 925 EP - 9 JF - Electrophoresis JO - Electrophoresis VL - 21 IS - 5 N2 - Capillary zone electrophoresis was employed for the determination of pipemidic acid using an end-column amperometric detection with a carbon fiber microdisk array electrode, at a constant potential of -1.10 V vs. saturated calomel electrode. The optimum conditions of separation and detection were 1.2 x 10(-4) mol/LNaOAc - 8.8 x 10(-4) mol/ LHOAc for the buffer solution, 20 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time. The limit of detection was 1.05 x 10(-7) mol/L or 189 amol (S/N=3). The relative standard deviation was 0.31% for the migration time and 2.0% for the electrophoretic peak current. The method was applied to determining pipemidic acid in human serum. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/10768778/A_new_method_of_quantification_of_pipemidic_acid_by_capillary_zone_electrophoresis_with_end_column_amperometric_detection_ L2 - https://doi.org/10.1002/(SICI)1522-2683(20000301)21:5<925::AID-ELPS925>3.0.CO;2-T DB - PRIME DP - Unbound Medicine ER -