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The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: a light and electron microscopic immunohistochemical study in the rat.
Neurosci Res. 2000 Apr; 36(4):327-34.NR

Abstract

The synaptic relationship between substance P (SP) and its receptor, i.e. neurokinin-1 receptor (NK1R), was examined in the superficial laminae of the caudal subnucleus of the spinal trigeminal nucleus (medullary dorsal horn; MDH) of the rat. For confocal laser-scanning microscopy, double-immunofluorescence histochemistry for NK1 and SP was performed. In electron microscopic double-immunolabeling study, immunoreactivity for NK1R was detected with the silver-intensified gold method, while immunoreactivity for SP was detected with peroxidase immunohistochemistry. SP-immunoreactive axon terminals were observed to be in synaptic (mostly asymmetric) contact with NK1R-immunoreactive neuronal profiles in lamina I and lamina IIo. Although some SP-immunoreactive axon terminals were in synaptic contact with NK1R-immunoreactive sites of plasma membranes, NK1R-immunoreactivity was observed at both synaptic and non-synaptic sites of plasma membrane. Thus, SP released from axon terminals might not only act on NK1Rs facing the SP-containing axon terminals, but also diffuse in the extracellular fluid for distances larger than the synaptic cleft to act on NK1Rs at some distances from the synaptic sites.

Authors+Show Affiliations

Department of Anatomy and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10771111

Citation

Li, J L., et al. "The Relationship Between Neurokinin-1 Receptor and Substance P in the Medullary Dorsal Horn: a Light and Electron Microscopic Immunohistochemical Study in the Rat." Neuroscience Research, vol. 36, no. 4, 2000, pp. 327-34.
Li JL, Wang D, Kaneko T, et al. The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: a light and electron microscopic immunohistochemical study in the rat. Neurosci Res. 2000;36(4):327-34.
Li, J. L., Wang, D., Kaneko, T., Shigemoto, R., Nomura, S., & Mizuno, N. (2000). The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: a light and electron microscopic immunohistochemical study in the rat. Neuroscience Research, 36(4), 327-34.
Li JL, et al. The Relationship Between Neurokinin-1 Receptor and Substance P in the Medullary Dorsal Horn: a Light and Electron Microscopic Immunohistochemical Study in the Rat. Neurosci Res. 2000;36(4):327-34. PubMed PMID: 10771111.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: a light and electron microscopic immunohistochemical study in the rat. AU - Li,J L, AU - Wang,D, AU - Kaneko,T, AU - Shigemoto,R, AU - Nomura,S, AU - Mizuno,N, PY - 2000/4/20/pubmed PY - 2000/6/10/medline PY - 2000/4/20/entrez SP - 327 EP - 34 JF - Neuroscience research JO - Neurosci. Res. VL - 36 IS - 4 N2 - The synaptic relationship between substance P (SP) and its receptor, i.e. neurokinin-1 receptor (NK1R), was examined in the superficial laminae of the caudal subnucleus of the spinal trigeminal nucleus (medullary dorsal horn; MDH) of the rat. For confocal laser-scanning microscopy, double-immunofluorescence histochemistry for NK1 and SP was performed. In electron microscopic double-immunolabeling study, immunoreactivity for NK1R was detected with the silver-intensified gold method, while immunoreactivity for SP was detected with peroxidase immunohistochemistry. SP-immunoreactive axon terminals were observed to be in synaptic (mostly asymmetric) contact with NK1R-immunoreactive neuronal profiles in lamina I and lamina IIo. Although some SP-immunoreactive axon terminals were in synaptic contact with NK1R-immunoreactive sites of plasma membranes, NK1R-immunoreactivity was observed at both synaptic and non-synaptic sites of plasma membrane. Thus, SP released from axon terminals might not only act on NK1Rs facing the SP-containing axon terminals, but also diffuse in the extracellular fluid for distances larger than the synaptic cleft to act on NK1Rs at some distances from the synaptic sites. SN - 0168-0102 UR - https://www.unboundmedicine.com/medline/citation/10771111/The_relationship_between_neurokinin_1_receptor_and_substance_P_in_the_medullary_dorsal_horn:_a_light_and_electron_microscopic_immunohistochemical_study_in_the_rat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S016801020000095X DB - PRIME DP - Unbound Medicine ER -