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Comparison among differential pulse voltammetry, amperometric biosensor, and HPLC/DAD analysis for polyphenol determination.
J Agric Food Chem. 2000 Apr; 48(4):1197-203.JA

Abstract

Polyphenols are widespread in vegetables and fruits. They can play an important role in human diet and health, and they influence the sensorial properties of many foods, and act as natural antioxidants. This study was conducted using HPLC/DAD, tyrosinase biosensor, and differential pulse voltammetry (DPV) analyses to detect polyphenolic compounds in natural complex matrices. The analyses were applied to a series of both standards and natural extracts derived from grape, olives, and green tea. The pure compounds include phenolic acids, flavones, flavonols, catechins, tannins, and oleuropein. HPLC/DAD, DPV, and the biosensor approach were used as independent analytical techniques. Bare graphite screen-printed electrodes were employed in DPV and in the biosensor analysis. The most accurate data were obtained by HPLC/DAD analysis, while the DPV approach using screen-printed electrodes could represent a quick screening method for the determination of polyphenols in natural extracts. Use of the biosensor for the analysis of complex matrices needs further study in order to improve its performance.

Authors+Show Affiliations

Dip. di Sanità Pubblica, Epidemiologia e Chimica Analitica Ambientale, sez. di Chimica Analitica, Via G. Capponi 9, 50121 Firenze, Italy. romani.a@farmfiscifarm.unifi.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10775372

Citation

Romani, A, et al. "Comparison Among Differential Pulse Voltammetry, Amperometric Biosensor, and HPLC/DAD Analysis for Polyphenol Determination." Journal of Agricultural and Food Chemistry, vol. 48, no. 4, 2000, pp. 1197-203.
Romani A, Minunni M, Mulinacci N, et al. Comparison among differential pulse voltammetry, amperometric biosensor, and HPLC/DAD analysis for polyphenol determination. J Agric Food Chem. 2000;48(4):1197-203.
Romani, A., Minunni, M., Mulinacci, N., Pinelli, P., Vincieri, F. F., Del Carlo, M., & Mascini, M. (2000). Comparison among differential pulse voltammetry, amperometric biosensor, and HPLC/DAD analysis for polyphenol determination. Journal of Agricultural and Food Chemistry, 48(4), 1197-203.
Romani A, et al. Comparison Among Differential Pulse Voltammetry, Amperometric Biosensor, and HPLC/DAD Analysis for Polyphenol Determination. J Agric Food Chem. 2000;48(4):1197-203. PubMed PMID: 10775372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison among differential pulse voltammetry, amperometric biosensor, and HPLC/DAD analysis for polyphenol determination. AU - Romani,A, AU - Minunni,M, AU - Mulinacci,N, AU - Pinelli,P, AU - Vincieri,F F, AU - Del Carlo,M, AU - Mascini,M, PY - 2000/4/25/pubmed PY - 2000/6/24/medline PY - 2000/4/25/entrez SP - 1197 EP - 203 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 48 IS - 4 N2 - Polyphenols are widespread in vegetables and fruits. They can play an important role in human diet and health, and they influence the sensorial properties of many foods, and act as natural antioxidants. This study was conducted using HPLC/DAD, tyrosinase biosensor, and differential pulse voltammetry (DPV) analyses to detect polyphenolic compounds in natural complex matrices. The analyses were applied to a series of both standards and natural extracts derived from grape, olives, and green tea. The pure compounds include phenolic acids, flavones, flavonols, catechins, tannins, and oleuropein. HPLC/DAD, DPV, and the biosensor approach were used as independent analytical techniques. Bare graphite screen-printed electrodes were employed in DPV and in the biosensor analysis. The most accurate data were obtained by HPLC/DAD analysis, while the DPV approach using screen-printed electrodes could represent a quick screening method for the determination of polyphenols in natural extracts. Use of the biosensor for the analysis of complex matrices needs further study in order to improve its performance. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/10775372/Comparison_among_differential_pulse_voltammetry_amperometric_biosensor_and_HPLC/DAD_analysis_for_polyphenol_determination_ L2 - https://doi.org/10.1021/jf990767e DB - PRIME DP - Unbound Medicine ER -