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Transport of alpha-ketoisocaproate in rat cerebral cortical neurons.
Arch Biochem Biophys. 2000 Apr 15; 376(2):347-53.AB

Abstract

Transport of alpha-ketoisocaproic acid (KIC), the product of leucine transamination, was studied in the cerebral cortex cells isolated from the adult rat brain. The process of [(14)C]KIC accumulation was followed in the presence of aminooxyacetate, an inhibitor of transaminases. Accumulation of KIC was not affected by Na(+) replacement, its initial velocity was observed to be higher upon lowering of external pH. Addition of KIC promoted acidification of cytoplasmic pH, monitored with 2'7'-bis(carboxyethyl)-5(6)-carboxyfluorescein. The detected inhibition of KIC accumulation by alpha-cyano-4(OH)cinnamate pointed to an involvement of one of monocarboxylate transporters (MCT), although 4,4'-diisothiocyano-2,2'-stilbenedisulphonate was without effect. Accumulation of KIC was inhibited by lactate; the effect of pyruvate was detected to be much weaker. Other branched-chain alpha-ketoacids (ketoisovalerate, keto-methylvalerate), as well as beta-hydroxybutyrate and valproate decreased the transport of KIC by 30, 60, and 80%, respectively. The observed characteristics of KIC accumulation in the cortical neurons indicate an involvement of one of the MCT transporters. A crucial role of SH group(s) in the process of KIC accumulation, excluding MCT2, indicates the MCT1, although an involvement of another isoform of MCT in the process of KIC transport in neurons from cerebral cortex of adult brain has not been definitely excluded.

Authors+Show Affiliations

Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Pasteur Street 3, Warsaw, 02-093, Poland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10775422

Citation

Mac, M, et al. "Transport of Alpha-ketoisocaproate in Rat Cerebral Cortical Neurons." Archives of Biochemistry and Biophysics, vol. 376, no. 2, 2000, pp. 347-53.
Mac M, Nehlig A, Nałecz MJ, et al. Transport of alpha-ketoisocaproate in rat cerebral cortical neurons. Arch Biochem Biophys. 2000;376(2):347-53.
Mac, M., Nehlig, A., Nałecz, M. J., & Nałecz, K. A. (2000). Transport of alpha-ketoisocaproate in rat cerebral cortical neurons. Archives of Biochemistry and Biophysics, 376(2), 347-53.
Mac M, et al. Transport of Alpha-ketoisocaproate in Rat Cerebral Cortical Neurons. Arch Biochem Biophys. 2000 Apr 15;376(2):347-53. PubMed PMID: 10775422.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transport of alpha-ketoisocaproate in rat cerebral cortical neurons. AU - Mac,M, AU - Nehlig,A, AU - Nałecz,M J, AU - Nałecz,K A, PY - 2000/4/25/pubmed PY - 2000/4/25/medline PY - 2000/4/25/entrez SP - 347 EP - 53 JF - Archives of biochemistry and biophysics JO - Arch Biochem Biophys VL - 376 IS - 2 N2 - Transport of alpha-ketoisocaproic acid (KIC), the product of leucine transamination, was studied in the cerebral cortex cells isolated from the adult rat brain. The process of [(14)C]KIC accumulation was followed in the presence of aminooxyacetate, an inhibitor of transaminases. Accumulation of KIC was not affected by Na(+) replacement, its initial velocity was observed to be higher upon lowering of external pH. Addition of KIC promoted acidification of cytoplasmic pH, monitored with 2'7'-bis(carboxyethyl)-5(6)-carboxyfluorescein. The detected inhibition of KIC accumulation by alpha-cyano-4(OH)cinnamate pointed to an involvement of one of monocarboxylate transporters (MCT), although 4,4'-diisothiocyano-2,2'-stilbenedisulphonate was without effect. Accumulation of KIC was inhibited by lactate; the effect of pyruvate was detected to be much weaker. Other branched-chain alpha-ketoacids (ketoisovalerate, keto-methylvalerate), as well as beta-hydroxybutyrate and valproate decreased the transport of KIC by 30, 60, and 80%, respectively. The observed characteristics of KIC accumulation in the cortical neurons indicate an involvement of one of the MCT transporters. A crucial role of SH group(s) in the process of KIC accumulation, excluding MCT2, indicates the MCT1, although an involvement of another isoform of MCT in the process of KIC transport in neurons from cerebral cortex of adult brain has not been definitely excluded. SN - 0003-9861 UR - https://www.unboundmedicine.com/medline/citation/10775422/Transport_of_alpha_ketoisocaproate_in_rat_cerebral_cortical_neurons_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9861(00)91724-7 DB - PRIME DP - Unbound Medicine ER -