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Antibody reactivity against human and guinea pig tissue transglutaminase in children with celiac disease.
J Pediatr Gastroenterol Nutr 2000; 30(4):379-84JP

Abstract

BACKGROUND

Highly discriminatory markers for celiac disease are needed to identify children with early mucosal lesions. The purposes of this study were to evaluate the clinical potential of circulating anti-tissue transglutaminase (tTG) immunoglobulin (Ig)A antibodies in the diagnosis of childhood celiac disease and to investigate the extent of autoreactivity of these antibodies.

METHODS

Included in this retrospective study were samples from 22 children with biopsy-verified celiac disease, 23 control subjects with disease, and 22 healthy control subjects without any known gastrointestinal or inflammatory disorders. An enzyme-linked immunosorbent assay (ELISA) was used to measure the serum levels of IgA antibodies specific for human and guinea pig tTGs. All samples were also analyzed for antibodies to gliadin and endomysium (EMA).

RESULTS

The concentrations of IgA specific for human and guinea pig tTGs correlated with the small intestinal villous structure and the serum levels of IgA EMA. The tTG ELISAs exhibited a high specificity and sensitivity for detection of untreated celiac disease. The human erythrocyte IgA tTG ELISA had the highest sensitivity (100%) and a specificity of 98%. The IgA EMA method had a sensitivity of 95% and the highest specificity (100%) of all tests.

CONCLUSIONS

Our results provide additional support to the concept that anti-tTG IgA antibodies can be used as a highly discriminatory serologic marker for celiac disease and that measurements of these autoreactive antibodies may in the future be used as an alternative to the EMA test.

Authors+Show Affiliations

Department of Clinical Immunology, Uppsala University Hospital, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10776947

Citation

Hansson, T, et al. "Antibody Reactivity Against Human and Guinea Pig Tissue Transglutaminase in Children With Celiac Disease." Journal of Pediatric Gastroenterology and Nutrition, vol. 30, no. 4, 2000, pp. 379-84.
Hansson T, Dahlbom I, Hall J, et al. Antibody reactivity against human and guinea pig tissue transglutaminase in children with celiac disease. J Pediatr Gastroenterol Nutr. 2000;30(4):379-84.
Hansson, T., Dahlbom, I., Hall, J., Holtz, A., Elfman, L., Dannaeus, A., & Klareskog, L. (2000). Antibody reactivity against human and guinea pig tissue transglutaminase in children with celiac disease. Journal of Pediatric Gastroenterology and Nutrition, 30(4), pp. 379-84.
Hansson T, et al. Antibody Reactivity Against Human and Guinea Pig Tissue Transglutaminase in Children With Celiac Disease. J Pediatr Gastroenterol Nutr. 2000;30(4):379-84. PubMed PMID: 10776947.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibody reactivity against human and guinea pig tissue transglutaminase in children with celiac disease. AU - Hansson,T, AU - Dahlbom,I, AU - Hall,J, AU - Holtz,A, AU - Elfman,L, AU - Dannaeus,A, AU - Klareskog,L, PY - 2000/4/25/pubmed PY - 2000/8/1/medline PY - 2000/4/25/entrez SP - 379 EP - 84 JF - Journal of pediatric gastroenterology and nutrition JO - J. Pediatr. Gastroenterol. Nutr. VL - 30 IS - 4 N2 - BACKGROUND: Highly discriminatory markers for celiac disease are needed to identify children with early mucosal lesions. The purposes of this study were to evaluate the clinical potential of circulating anti-tissue transglutaminase (tTG) immunoglobulin (Ig)A antibodies in the diagnosis of childhood celiac disease and to investigate the extent of autoreactivity of these antibodies. METHODS: Included in this retrospective study were samples from 22 children with biopsy-verified celiac disease, 23 control subjects with disease, and 22 healthy control subjects without any known gastrointestinal or inflammatory disorders. An enzyme-linked immunosorbent assay (ELISA) was used to measure the serum levels of IgA antibodies specific for human and guinea pig tTGs. All samples were also analyzed for antibodies to gliadin and endomysium (EMA). RESULTS: The concentrations of IgA specific for human and guinea pig tTGs correlated with the small intestinal villous structure and the serum levels of IgA EMA. The tTG ELISAs exhibited a high specificity and sensitivity for detection of untreated celiac disease. The human erythrocyte IgA tTG ELISA had the highest sensitivity (100%) and a specificity of 98%. The IgA EMA method had a sensitivity of 95% and the highest specificity (100%) of all tests. CONCLUSIONS: Our results provide additional support to the concept that anti-tTG IgA antibodies can be used as a highly discriminatory serologic marker for celiac disease and that measurements of these autoreactive antibodies may in the future be used as an alternative to the EMA test. SN - 0277-2116 UR - https://www.unboundmedicine.com/medline/citation/10776947/Antibody_reactivity_against_human_and_guinea_pig_tissue_transglutaminase_in_children_with_celiac_disease_ L2 - http://Insights.ovid.com/pubmed?pmid=10776947 DB - PRIME DP - Unbound Medicine ER -