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Effects of organic and inorganic selenium supplementation on selenoenzyme activity in blood lymphocytes, granulocytes, platelets and erythrocytes.

Abstract

The blood selenium (Se) concentration in the U.K. population has declined by approx. 50% between 1974 and 1991, reflecting a large decrease in dietary Se supply, with intakes only half the reference nutrient intake of 1 microg/kg body weight. Tissue levels of Se are readily influenced by dietary intake. Therefore selenoprotein activity may be sub-optimal due to low Se status, and thus compromise normal cell function. To examine the effects of changing Se intake on selenoproteins, we have determined the relative effectiveness of organic selenomethionine and inorganic sodium selenite (50 microg of Se daily for 28 days) in modulating glutathione peroxidase activities in blood cells from 45 healthy men and women, from a U.K. population. Transient and acute changes in lymphocyte, granulocyte and platelet phospholipid-hydroperoxide glutathione peroxidase (GPx4) activity occurred by day 7 or 14 of sodium selenite treatment and by day 7 in lymphocytes from selenomethionine-treated subjects compared with controls taking a placebo. In contrast, GPx4 activity in granulocytes and platelets in the selenomethionine group increased gradually over the 28 days. Cytosolic glutathione peroxidase (GPx1) activity in these blood cells from both treatment groups increased gradually over the 28 days. For each cellular selenoenzyme activity a significant inter-individual difference (P<0.001) in the extent of the response to Se supplementation was observed, but this was not related to blood Se concentrations either before or after treatments. Significant inverse correlations were evident between baseline enzyme activities and percentage change in activity after 28 days of supplementation [e.g. lymphocyte GPx4, r=-0.695 (P<0.001)], indicating that pre-treatment activity may be sub-optimal as a result of poor Se status. The different and contrasting effects that Se supplementation had on blood selenoenzyme activities may be indicative of a difference in metabolic need for Se regulated at the level of Se-dependent cell function.

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  • Authors+Show Affiliations

    ,

    Rowett Research Institute, Division of Micronutrient and Lipid Metabolism, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, Scotland, U.K. k.m.brown@abdn.ac.uk

    , , , ,

    Source

    MeSH

    Adult
    Blood Cells
    Blood Platelets
    Dietary Supplements
    Erythrocytes
    Female
    Glutathione Peroxidase
    Granulocytes
    Humans
    Lymphocytes
    Male
    Middle Aged
    Selenium
    Selenomethionine
    Sodium Selenite

    Pub Type(s)

    Clinical Trial
    Journal Article
    Randomized Controlled Trial
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    10781391

    Citation

    Brown, K M., et al. "Effects of Organic and Inorganic Selenium Supplementation On Selenoenzyme Activity in Blood Lymphocytes, Granulocytes, Platelets and Erythrocytes." Clinical Science (London, England : 1979), vol. 98, no. 5, 2000, pp. 593-9.
    Brown KM, Pickard K, Nicol F, et al. Effects of organic and inorganic selenium supplementation on selenoenzyme activity in blood lymphocytes, granulocytes, platelets and erythrocytes. Clin Sci. 2000;98(5):593-9.
    Brown, K. M., Pickard, K., Nicol, F., Beckett, G. J., Duthie, G. G., & Arthur, J. R. (2000). Effects of organic and inorganic selenium supplementation on selenoenzyme activity in blood lymphocytes, granulocytes, platelets and erythrocytes. Clinical Science (London, England : 1979), 98(5), pp. 593-9.
    Brown KM, et al. Effects of Organic and Inorganic Selenium Supplementation On Selenoenzyme Activity in Blood Lymphocytes, Granulocytes, Platelets and Erythrocytes. Clin Sci. 2000;98(5):593-9. PubMed PMID: 10781391.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effects of organic and inorganic selenium supplementation on selenoenzyme activity in blood lymphocytes, granulocytes, platelets and erythrocytes. AU - Brown,K M, AU - Pickard,K, AU - Nicol,F, AU - Beckett,G J, AU - Duthie,G G, AU - Arthur,J R, PY - 2000/4/27/pubmed PY - 2000/6/17/medline PY - 2000/4/27/entrez SP - 593 EP - 9 JF - Clinical science (London, England : 1979) JO - Clin. Sci. VL - 98 IS - 5 N2 - The blood selenium (Se) concentration in the U.K. population has declined by approx. 50% between 1974 and 1991, reflecting a large decrease in dietary Se supply, with intakes only half the reference nutrient intake of 1 microg/kg body weight. Tissue levels of Se are readily influenced by dietary intake. Therefore selenoprotein activity may be sub-optimal due to low Se status, and thus compromise normal cell function. To examine the effects of changing Se intake on selenoproteins, we have determined the relative effectiveness of organic selenomethionine and inorganic sodium selenite (50 microg of Se daily for 28 days) in modulating glutathione peroxidase activities in blood cells from 45 healthy men and women, from a U.K. population. Transient and acute changes in lymphocyte, granulocyte and platelet phospholipid-hydroperoxide glutathione peroxidase (GPx4) activity occurred by day 7 or 14 of sodium selenite treatment and by day 7 in lymphocytes from selenomethionine-treated subjects compared with controls taking a placebo. In contrast, GPx4 activity in granulocytes and platelets in the selenomethionine group increased gradually over the 28 days. Cytosolic glutathione peroxidase (GPx1) activity in these blood cells from both treatment groups increased gradually over the 28 days. For each cellular selenoenzyme activity a significant inter-individual difference (P<0.001) in the extent of the response to Se supplementation was observed, but this was not related to blood Se concentrations either before or after treatments. Significant inverse correlations were evident between baseline enzyme activities and percentage change in activity after 28 days of supplementation [e.g. lymphocyte GPx4, r=-0.695 (P<0.001)], indicating that pre-treatment activity may be sub-optimal as a result of poor Se status. The different and contrasting effects that Se supplementation had on blood selenoenzyme activities may be indicative of a difference in metabolic need for Se regulated at the level of Se-dependent cell function. SN - 0143-5221 UR - https://www.unboundmedicine.com/medline/citation/10781391/Effects_of_organic_and_inorganic_selenium_supplementation_on_selenoenzyme_activity_in_blood_lymphocytes_granulocytes_platelets_and_erythrocytes_ L2 - http://clinsci.org/cgi/pmidlookup?view=long&amp;pmid=10781391 DB - PRIME DP - Unbound Medicine ER -