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Pituitary adenylate cyclase activating peptide mediates inhibitory nonadrenergic noncholinergic relaxation.
Eur J Pharmacol. 2000 Apr 21; 395(1):77-83.EJ

Abstract

We investigated the contribution of pituitary adenylate cyclase activating peptide (PACAP) to inhibitory nonadrenergic noncholinergic (inhibitory-NANC) relaxation of tracheal smooth muscle in cats. We also investigated the roles of vasoactive intestinal peptide (VIP) and nitric oxide (NO) on this function. Smooth muscle strips prepared from feline trachea were precontracted with 1 microM serotonin, and inhibitory-NANC relaxation was induced by electrical-field stimulation in the presence of atropine and propranolol. PACAP-(6-38) (a selective antagonist of PACAP; 1, 3 and 10 microM), VIP-(10-28) (a selective antagonist of VIP; 1, 3 and 10 microM) and N(omega)-nitro-L-arginine methyl ester (L-NAME, a selective NO synthase inhibitor; 3, 10 and 30 microM) each partially but significantly attenuated the amplitude of inhibitory-NANC relaxation. The effects of PACAP-(6-38) and VIP-(10-28) were additive. Addition of PACAP-(6-38) and/or VIP-(10-28) further attenuated relaxation in the presence of L-NAME. These results suggest that PACAP, VIP and NO contribute to the relaxation induced by inhibitory-NANC in tracheal smooth muscle in cats, and that they mediate this relaxation via different pathways.

Authors+Show Affiliations

Faculty of Medicine, Research Institute for Diseases of the Chest, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka, Japan. yoshidam@kokyu.med.kyushu-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10781677

Citation

Yoshida, M, et al. "Pituitary Adenylate Cyclase Activating Peptide Mediates Inhibitory Nonadrenergic Noncholinergic Relaxation." European Journal of Pharmacology, vol. 395, no. 1, 2000, pp. 77-83.
Yoshida M, Aizawa H, Takahashi N, et al. Pituitary adenylate cyclase activating peptide mediates inhibitory nonadrenergic noncholinergic relaxation. Eur J Pharmacol. 2000;395(1):77-83.
Yoshida, M., Aizawa, H., Takahashi, N., Shigyo, M., & Hara, N. (2000). Pituitary adenylate cyclase activating peptide mediates inhibitory nonadrenergic noncholinergic relaxation. European Journal of Pharmacology, 395(1), 77-83.
Yoshida M, et al. Pituitary Adenylate Cyclase Activating Peptide Mediates Inhibitory Nonadrenergic Noncholinergic Relaxation. Eur J Pharmacol. 2000 Apr 21;395(1):77-83. PubMed PMID: 10781677.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pituitary adenylate cyclase activating peptide mediates inhibitory nonadrenergic noncholinergic relaxation. AU - Yoshida,M, AU - Aizawa,H, AU - Takahashi,N, AU - Shigyo,M, AU - Hara,N, PY - 2000/4/27/pubmed PY - 2000/7/6/medline PY - 2000/4/27/entrez SP - 77 EP - 83 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 395 IS - 1 N2 - We investigated the contribution of pituitary adenylate cyclase activating peptide (PACAP) to inhibitory nonadrenergic noncholinergic (inhibitory-NANC) relaxation of tracheal smooth muscle in cats. We also investigated the roles of vasoactive intestinal peptide (VIP) and nitric oxide (NO) on this function. Smooth muscle strips prepared from feline trachea were precontracted with 1 microM serotonin, and inhibitory-NANC relaxation was induced by electrical-field stimulation in the presence of atropine and propranolol. PACAP-(6-38) (a selective antagonist of PACAP; 1, 3 and 10 microM), VIP-(10-28) (a selective antagonist of VIP; 1, 3 and 10 microM) and N(omega)-nitro-L-arginine methyl ester (L-NAME, a selective NO synthase inhibitor; 3, 10 and 30 microM) each partially but significantly attenuated the amplitude of inhibitory-NANC relaxation. The effects of PACAP-(6-38) and VIP-(10-28) were additive. Addition of PACAP-(6-38) and/or VIP-(10-28) further attenuated relaxation in the presence of L-NAME. These results suggest that PACAP, VIP and NO contribute to the relaxation induced by inhibitory-NANC in tracheal smooth muscle in cats, and that they mediate this relaxation via different pathways. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/10781677/Pituitary_adenylate_cyclase_activating_peptide_mediates_inhibitory_nonadrenergic_noncholinergic_relaxation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(00)00167-9 DB - PRIME DP - Unbound Medicine ER -