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Redirection of the respiro-fermentative flux distribution in Saccharomyces cerevisiae by overexpression of the transcription factor Hap4p.
Appl Environ Microbiol. 2000 May; 66(5):1970-3.AE

Abstract

Reduction of aerobic fermentation on sugars by altering the fermentative/oxidative balance is of significant interest for optimization of industrial production of Saccharomyces cerevisiae. Glucose control of oxidative metabolism in baker's yeast is partly mediated through transcriptional regulation of the Hap4p subunit of the Hap2/3/4/5p transcriptional activator complex. To alleviate glucose repression of oxidative metabolism, we constructed a yeast strain with constitutively elevated levels of Hap4p. Genetic analysis of expression levels of glucose-repressed genes and analysis of respiratory capacity showed that Hap4p overexpression (partly) relieves glucose repression of respiration. Analysis of the physiological properties of the Hap4p overproducer in batch cultures in fermentors (aerobic, glucose excess) has shown that the metabolism of this strain is more oxidative than in the wild-type strain, resulting in a significant reduced ethanol production and improvement of growth rate and a 40% gain in biomass yield. Our results show that modification of one or more transcriptional regulators can be a powerful and a widely applicable tool for redirection of metabolic fluxes in microorganisms.

Authors+Show Affiliations

Section for Molecular Biology, Swammerdam Institute of Life Sciences, University of Amsterdam, 1098 SM Amsterdam, The Netherlands.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10788368

Citation

Blom, J, et al. "Redirection of the Respiro-fermentative Flux Distribution in Saccharomyces Cerevisiae By Overexpression of the Transcription Factor Hap4p." Applied and Environmental Microbiology, vol. 66, no. 5, 2000, pp. 1970-3.
Blom J, De Mattos MJ, Grivell LA. Redirection of the respiro-fermentative flux distribution in Saccharomyces cerevisiae by overexpression of the transcription factor Hap4p. Appl Environ Microbiol. 2000;66(5):1970-3.
Blom, J., De Mattos, M. J., & Grivell, L. A. (2000). Redirection of the respiro-fermentative flux distribution in Saccharomyces cerevisiae by overexpression of the transcription factor Hap4p. Applied and Environmental Microbiology, 66(5), 1970-3.
Blom J, De Mattos MJ, Grivell LA. Redirection of the Respiro-fermentative Flux Distribution in Saccharomyces Cerevisiae By Overexpression of the Transcription Factor Hap4p. Appl Environ Microbiol. 2000;66(5):1970-3. PubMed PMID: 10788368.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Redirection of the respiro-fermentative flux distribution in Saccharomyces cerevisiae by overexpression of the transcription factor Hap4p. AU - Blom,J, AU - De Mattos,M J, AU - Grivell,L A, PY - 2000/5/2/pubmed PY - 2000/6/24/medline PY - 2000/5/2/entrez SP - 1970 EP - 3 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 66 IS - 5 N2 - Reduction of aerobic fermentation on sugars by altering the fermentative/oxidative balance is of significant interest for optimization of industrial production of Saccharomyces cerevisiae. Glucose control of oxidative metabolism in baker's yeast is partly mediated through transcriptional regulation of the Hap4p subunit of the Hap2/3/4/5p transcriptional activator complex. To alleviate glucose repression of oxidative metabolism, we constructed a yeast strain with constitutively elevated levels of Hap4p. Genetic analysis of expression levels of glucose-repressed genes and analysis of respiratory capacity showed that Hap4p overexpression (partly) relieves glucose repression of respiration. Analysis of the physiological properties of the Hap4p overproducer in batch cultures in fermentors (aerobic, glucose excess) has shown that the metabolism of this strain is more oxidative than in the wild-type strain, resulting in a significant reduced ethanol production and improvement of growth rate and a 40% gain in biomass yield. Our results show that modification of one or more transcriptional regulators can be a powerful and a widely applicable tool for redirection of metabolic fluxes in microorganisms. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/10788368/Redirection_of_the_respiro_fermentative_flux_distribution_in_Saccharomyces_cerevisiae_by_overexpression_of_the_transcription_factor_Hap4p_ DB - PRIME DP - Unbound Medicine ER -