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Two-dimensional gel analysis of the proteome of lager brewing yeasts.
Yeast. 2000 Apr; 16(6):511-22.Y

Abstract

Modern lager brewing yeasts used in beer production are hybrid strains consisting of at least two different genomes. To obtain information on the identity of the parental strains that gave rise to industrial lager yeasts, we used two-dimensional (2-D) gel electrophoresis and analysed the proteomes of different Saccharomyces species isolated from breweries. We found that the proteome of lager brewing yeasts and of the type strains of S. carlsbergensis, S. monacensis and S. pastorianus can be interpreted as the superimposition of two elementary patterns. One originates from proteins encoded by a S. cerevisiae-like genome. The other corresponds to a divergent Saccharomyces species whose best representative is a particular S. pastorianus strain, NRRL Y-1551. A map of industrial lager brewing yeasts has been established, with the individual origin of proteins and with identification of protein spots by comparison to known S. cerevisiae proteins. This 2-D map can be accessed on the Lager Brewing Yeast Protein Map server through the World Wide Web. This study provides the first example of the use of proteome analysis for investigating taxonomic relationships between divergent yeast species.

Authors+Show Affiliations

Joubert R
Institut de Biochimie et Génétique Cellulaires, UPR CNRS 9026, 1 rue Camille Saint-Saëns, F-33077, Bordeaux Cedex, France.
Brignon P
No affiliation info available
Lehmann C
No affiliation info available
Monribot C
No affiliation info available
Gendre F
No affiliation info available
Boucherie H
No affiliation info available

MeSH

Amino Acid SequenceChromosome MappingChromosomes, FungalElectrophoresis, Gel, Two-DimensionalEvolution, MolecularFungal ProteinsGenes, FungalGenome, FungalInternetMolecular Sequence DataProtein IsoformsSaccharomycesSaccharomyces cerevisiaeSequence Analysis, ProteinSequence Homology, Amino AcidSpecies Specificity

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10790688

Citation

Joubert, R, et al. "Two-dimensional Gel Analysis of the Proteome of Lager Brewing Yeasts." Yeast (Chichester, England), vol. 16, no. 6, 2000, pp. 511-22.
Joubert R, Brignon P, Lehmann C, et al. Two-dimensional gel analysis of the proteome of lager brewing yeasts. Yeast. 2000;16(6):511-22.
Joubert, R., Brignon, P., Lehmann, C., Monribot, C., Gendre, F., & Boucherie, H. (2000). Two-dimensional gel analysis of the proteome of lager brewing yeasts. Yeast (Chichester, England), 16(6), 511-22.
Joubert R, et al. Two-dimensional Gel Analysis of the Proteome of Lager Brewing Yeasts. Yeast. 2000;16(6):511-22. PubMed PMID: 10790688.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two-dimensional gel analysis of the proteome of lager brewing yeasts. AU - Joubert,R, AU - Brignon,P, AU - Lehmann,C, AU - Monribot,C, AU - Gendre,F, AU - Boucherie,H, PY - 2000/5/3/pubmed PY - 2000/6/10/medline PY - 2000/5/3/entrez SP - 511 EP - 22 JF - Yeast (Chichester, England) JO - Yeast VL - 16 IS - 6 N2 - Modern lager brewing yeasts used in beer production are hybrid strains consisting of at least two different genomes. To obtain information on the identity of the parental strains that gave rise to industrial lager yeasts, we used two-dimensional (2-D) gel electrophoresis and analysed the proteomes of different Saccharomyces species isolated from breweries. We found that the proteome of lager brewing yeasts and of the type strains of S. carlsbergensis, S. monacensis and S. pastorianus can be interpreted as the superimposition of two elementary patterns. One originates from proteins encoded by a S. cerevisiae-like genome. The other corresponds to a divergent Saccharomyces species whose best representative is a particular S. pastorianus strain, NRRL Y-1551. A map of industrial lager brewing yeasts has been established, with the individual origin of proteins and with identification of protein spots by comparison to known S. cerevisiae proteins. This 2-D map can be accessed on the Lager Brewing Yeast Protein Map server through the World Wide Web. This study provides the first example of the use of proteome analysis for investigating taxonomic relationships between divergent yeast species. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/10790688/Two_dimensional_gel_analysis_of_the_proteome_of_lager_brewing_yeasts_ L2 - https://doi.org/10.1002/(SICI)1097-0061(200004)16:6<511::AID-YEA544>3.0.CO;2-I DB - PRIME DP - Unbound Medicine ER -