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Cyclosporine treatment of RPE allografts in the rabbit subretinal space.
Acta Ophthalmol Scand. 2000 Apr; 78(2):122-9.AO

Abstract

PURPOSE

To determine the effects of systemic cyclosporine A (CsA) on the survival of retinal pigment epithelial (RPE) allografts in the subretinal space in an animal model using atraumatic transplantation surgery.

METHODS

Following pars plana vitrectomy, an RPE cell suspension from brown rabbits was injected with a glass micropipette into the subretinal space of 39 albino rabbits. For immunosuppression, 22 rabbits were given an injection of CsA, 20 mg daily intramuscularly, 17 rabbits with RPE grafts were controls. The grafts were monitored by biomicroscopy, color fundus photography, and fluorescein angiography. Rabbits were sacrificed at 1, 3 and 6 months, respectively, and the eyes processed for light and electron microscopy including immunohistochemistry.

RESULTS

After three months, the transplanted RPE cells, in both the CsA group and the controls, formed a monolayer in the subretinal space. Although a few macrophages were encountered, there was no massive cellular infiltration and the photoreceptor layer was well preserved. After six months, however, there was a disruption of grafted RPE cells in both groups, characterized by dispersion of melanin pigment in the subretinal space, and invasion of macrophages with focal photoreceptor damage but no infiltration of lymphocytes in the retina or choroid. No significant differences between the CsA treated and the control eyes were discernible.

CONCLUSION

Although the subretinal space has been considered an immunologically privileged site, we found that the survival of RPE allografts was limited. CsA did not prevent RPE allograft destruction in the subretinal space. The transplant seems to be disrupted either by immunological mechanisms that are not inhibited by CsA, or by nonimmunologic events.

Authors+Show Affiliations

Department of Ophthalmology, Orebro Medical Center, Sweden. sven.crafoord@orebroll.seNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10794242

Citation

Crafoord, S, et al. "Cyclosporine Treatment of RPE Allografts in the Rabbit Subretinal Space." Acta Ophthalmologica Scandinavica, vol. 78, no. 2, 2000, pp. 122-9.
Crafoord S, Algvere PV, Kopp ED, et al. Cyclosporine treatment of RPE allografts in the rabbit subretinal space. Acta Ophthalmol Scand. 2000;78(2):122-9.
Crafoord, S., Algvere, P. V., Kopp, E. D., & Seregard, S. (2000). Cyclosporine treatment of RPE allografts in the rabbit subretinal space. Acta Ophthalmologica Scandinavica, 78(2), 122-9.
Crafoord S, et al. Cyclosporine Treatment of RPE Allografts in the Rabbit Subretinal Space. Acta Ophthalmol Scand. 2000;78(2):122-9. PubMed PMID: 10794242.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cyclosporine treatment of RPE allografts in the rabbit subretinal space. AU - Crafoord,S, AU - Algvere,P V, AU - Kopp,E D, AU - Seregard,S, PY - 2000/5/4/pubmed PY - 2000/6/10/medline PY - 2000/5/4/entrez SP - 122 EP - 9 JF - Acta ophthalmologica Scandinavica JO - Acta Ophthalmol Scand VL - 78 IS - 2 N2 - PURPOSE: To determine the effects of systemic cyclosporine A (CsA) on the survival of retinal pigment epithelial (RPE) allografts in the subretinal space in an animal model using atraumatic transplantation surgery. METHODS: Following pars plana vitrectomy, an RPE cell suspension from brown rabbits was injected with a glass micropipette into the subretinal space of 39 albino rabbits. For immunosuppression, 22 rabbits were given an injection of CsA, 20 mg daily intramuscularly, 17 rabbits with RPE grafts were controls. The grafts were monitored by biomicroscopy, color fundus photography, and fluorescein angiography. Rabbits were sacrificed at 1, 3 and 6 months, respectively, and the eyes processed for light and electron microscopy including immunohistochemistry. RESULTS: After three months, the transplanted RPE cells, in both the CsA group and the controls, formed a monolayer in the subretinal space. Although a few macrophages were encountered, there was no massive cellular infiltration and the photoreceptor layer was well preserved. After six months, however, there was a disruption of grafted RPE cells in both groups, characterized by dispersion of melanin pigment in the subretinal space, and invasion of macrophages with focal photoreceptor damage but no infiltration of lymphocytes in the retina or choroid. No significant differences between the CsA treated and the control eyes were discernible. CONCLUSION: Although the subretinal space has been considered an immunologically privileged site, we found that the survival of RPE allografts was limited. CsA did not prevent RPE allograft destruction in the subretinal space. The transplant seems to be disrupted either by immunological mechanisms that are not inhibited by CsA, or by nonimmunologic events. SN - 1395-3907 UR - https://www.unboundmedicine.com/medline/citation/10794242/Cyclosporine_treatment_of_RPE_allografts_in_the_rabbit_subretinal_space_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1395-3907&date=2000&volume=78&issue=2&spage=122 DB - PRIME DP - Unbound Medicine ER -