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2,2-Diphenyl-1-picrylhydrazyl radical-scavenging active components from Polygonum multiflorum thunb.
J Agric Food Chem. 1999 Jun; 47(6):2226-8.JA

Abstract

An activity-directed fractionation and purification process was used to identify the antioxidative components of Polygonum multiflorum Thunb. (PM). Dried root of PM was extracted with 95% ethanol and then separated into water, ethyl acetate, and hexane fractions. Among these only the ethyl acetate phase showed strong antioxidant activity by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test when compared with water and hexane phases. The ethyl acetate fraction was then subjected to separation and purification using silica gel column chromatography and Sephadex LH-20 chromatography. Three compounds showing strong antioxidant activity were identified by spectral methods ((1)H NMR, (13)C NMR, and MS) and by comparison with authentic samples to be gallic acid, catechin, and 2,3,5, 4'-tetrahydroxystilbene 2-O-beta-D-glucopyranoside.

Authors+Show Affiliations

Chen Y
Department of Food Science, Cook College, Rutgers, The State University of New Jersey, New Brunswick 08901-8520, USA.
Wang M
No affiliation info available
Rosen RT
No affiliation info available
Ho CT
No affiliation info available

MeSH

AntioxidantsBepridilBiphenyl CompoundsFree Radical ScavengersFree RadicalsPicratesPlant RootsPlants, Medicinal

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10794614

Citation

Chen, Y, et al. "2,2-Diphenyl-1-picrylhydrazyl Radical-scavenging Active Components From Polygonum Multiflorum Thunb." Journal of Agricultural and Food Chemistry, vol. 47, no. 6, 1999, pp. 2226-8.
Chen Y, Wang M, Rosen RT, et al. 2,2-Diphenyl-1-picrylhydrazyl radical-scavenging active components from Polygonum multiflorum thunb. J Agric Food Chem. 1999;47(6):2226-8.
Chen, Y., Wang, M., Rosen, R. T., & Ho, C. T. (1999). 2,2-Diphenyl-1-picrylhydrazyl radical-scavenging active components from Polygonum multiflorum thunb. Journal of Agricultural and Food Chemistry, 47(6), 2226-8.
Chen Y, et al. 2,2-Diphenyl-1-picrylhydrazyl Radical-scavenging Active Components From Polygonum Multiflorum Thunb. J Agric Food Chem. 1999;47(6):2226-8. PubMed PMID: 10794614.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 2,2-Diphenyl-1-picrylhydrazyl radical-scavenging active components from Polygonum multiflorum thunb. AU - Chen,Y, AU - Wang,M, AU - Rosen,R T, AU - Ho,C T, PY - 2000/5/4/pubmed PY - 2000/8/19/medline PY - 2000/5/4/entrez SP - 2226 EP - 8 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 47 IS - 6 N2 - An activity-directed fractionation and purification process was used to identify the antioxidative components of Polygonum multiflorum Thunb. (PM). Dried root of PM was extracted with 95% ethanol and then separated into water, ethyl acetate, and hexane fractions. Among these only the ethyl acetate phase showed strong antioxidant activity by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test when compared with water and hexane phases. The ethyl acetate fraction was then subjected to separation and purification using silica gel column chromatography and Sephadex LH-20 chromatography. Three compounds showing strong antioxidant activity were identified by spectral methods ((1)H NMR, (13)C NMR, and MS) and by comparison with authentic samples to be gallic acid, catechin, and 2,3,5, 4'-tetrahydroxystilbene 2-O-beta-D-glucopyranoside. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/10794614/22_Diphenyl_1_picrylhydrazyl_radical_scavenging_active_components_from_Polygonum_multiflorum_thunb_ L2 - https://doi.org/10.1021/jf990092f DB - PRIME DP - Unbound Medicine ER -