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Distribution of P2X receptors in the urinary bladder and the ureter of the rat.
J Urol. 2000 Jun; 163(6):2002-7.JU

Abstract

PURPOSE

One means of assessing the presence of purinoceptors and their possible participation in signaling events in tissues is through use of specific antibodies and immunohistochemical methods. A thorough immunohistochemical screening for the presence of P2X receptors on bladder and ureter sections has been performed.

MATERIALS AND METHODS

Distribution of P2X receptor subtypes in rat bladder and ureter has been investigated using specific polyclonal antibodies to P2X1 through to P2X7 receptor subtypes with immunohistochemical methods.

RESULTS

In both the bladder detrusor muscle and the ureteral muscle (as well as the accompanying arteries) P2X1 immunoreactivity was associated with the smooth muscle membranes. Non-membrane associated smooth muscle reactivity was seen with P2X2> P2X5 = P2X6. P2X3 immunoreactivity was seen within nerve bundles in detrusor muscle only. The fine capillary network supplying bladder and ureter smooth muscle and lamina propria was visualized with P2X4 immunoreactivity, membranes of urothelial cells gave a strong reaction with P2X5, whereas P2X6 immunostained the thin basement membrane beneath the urothelium. Nuclear staining was seen with P2X7 in the urothelium but more prominent in the bladder than in the ureter.

CONCLUSIONS

Having established the distribution of P2X receptors in normal animal bladder and ureter tissue, it is now possible to perform comparable investigations on normal and diseased human tissue to establish a possible role of P2X receptors in pathogenic events.

Authors+Show Affiliations

Autonomic Neuroscience Institute, Royal Free and University College Medical School, Rowland Hill Street, London, United Kingdom.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10799247

Citation

Lee, H Y., et al. "Distribution of P2X Receptors in the Urinary Bladder and the Ureter of the Rat." The Journal of Urology, vol. 163, no. 6, 2000, pp. 2002-7.
Lee HY, Bardini M, Burnstock G. Distribution of P2X receptors in the urinary bladder and the ureter of the rat. J Urol. 2000;163(6):2002-7.
Lee, H. Y., Bardini, M., & Burnstock, G. (2000). Distribution of P2X receptors in the urinary bladder and the ureter of the rat. The Journal of Urology, 163(6), 2002-7.
Lee HY, Bardini M, Burnstock G. Distribution of P2X Receptors in the Urinary Bladder and the Ureter of the Rat. J Urol. 2000;163(6):2002-7. PubMed PMID: 10799247.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of P2X receptors in the urinary bladder and the ureter of the rat. AU - Lee,H Y, AU - Bardini,M, AU - Burnstock,G, PY - 2000/5/8/pubmed PY - 2000/7/6/medline PY - 2000/5/8/entrez SP - 2002 EP - 7 JF - The Journal of urology JO - J Urol VL - 163 IS - 6 N2 - PURPOSE: One means of assessing the presence of purinoceptors and their possible participation in signaling events in tissues is through use of specific antibodies and immunohistochemical methods. A thorough immunohistochemical screening for the presence of P2X receptors on bladder and ureter sections has been performed. MATERIALS AND METHODS: Distribution of P2X receptor subtypes in rat bladder and ureter has been investigated using specific polyclonal antibodies to P2X1 through to P2X7 receptor subtypes with immunohistochemical methods. RESULTS: In both the bladder detrusor muscle and the ureteral muscle (as well as the accompanying arteries) P2X1 immunoreactivity was associated with the smooth muscle membranes. Non-membrane associated smooth muscle reactivity was seen with P2X2> P2X5 = P2X6. P2X3 immunoreactivity was seen within nerve bundles in detrusor muscle only. The fine capillary network supplying bladder and ureter smooth muscle and lamina propria was visualized with P2X4 immunoreactivity, membranes of urothelial cells gave a strong reaction with P2X5, whereas P2X6 immunostained the thin basement membrane beneath the urothelium. Nuclear staining was seen with P2X7 in the urothelium but more prominent in the bladder than in the ureter. CONCLUSIONS: Having established the distribution of P2X receptors in normal animal bladder and ureter tissue, it is now possible to perform comparable investigations on normal and diseased human tissue to establish a possible role of P2X receptors in pathogenic events. SN - 0022-5347 UR - https://www.unboundmedicine.com/medline/citation/10799247/Distribution_of_P2X_receptors_in_the_urinary_bladder_and_the_ureter_of_the_rat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-5347(05)67618-5 DB - PRIME DP - Unbound Medicine ER -