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Canine antibody response to Blastomyces dermatitidis WI-1 antigen.
Am J Vet Res. 2000 May; 61(5):554-8.AJ

Abstract

OBJECTIVE

To assess whether dogs with blastomycosis produce antibodies against the WI-1 and A-antigens of Blastomyces dermatitidis and whether the antibodies are useful in serodiagnosis.

SAMPLE POPULATION

359 serum samples obtained from 245 dogs.

PROCEDURE

233 samples from 122 dogs with blastomycosis, and 1 sample each from 24 dogs with suspected blastomycosis, 51 control dogs without infection, and 48 healthy dogs from an enzootic region were obtained. Antibodies against WI-1 antigen were detected by radioimmunoassay (RIA). Serum samples were tested in parallel for antibodies against the A-antigen of B dermatitidis by commercial agar-gel immunodiffusion (AGID) in a reference laboratory.

RESULTS

Antibodies were detected in 92% of infected dogs by RIA and in 41 % by AGID. For 29 serum samples that were obtained 11 to 1,545 days after diagnosis, antibodies were detected in 92% of samples by RIA and 7% by AGID. For 93 serial serum samples from 29 dogs with blastomycosis, the mean anti-WI-1 titer was 1:18,761 at the time of diagnosis, and decreased to a mean of 1:1,338 by 210 days after treatment was initiated. Of 24 dogs with suspected infection, antibodies were detected in 67% by RIA and 33% by AGID. Control dogs without blastomycosis had no detectable antibodies in either assay. Thus, sensitivity was 92% for RIA and 41 % for AGID, and specificity was 100% for both tests.

CONCLUSIONS AND CLINICAL RELEVANCE

Anti-WI-1 antibodies are readily detected by RIA in dogs with blastomycosis. Titers become high, decline during treatment, and persist for months. Anti-A antibodies are sometimes detected with AGID, but these decrease quickly.

Authors+Show Affiliations

Department of Internal Medicine, University of Wisconsin Medical School, Madison 53792, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10803652

Citation

Klein, B S., et al. "Canine Antibody Response to Blastomyces Dermatitidis WI-1 Antigen." American Journal of Veterinary Research, vol. 61, no. 5, 2000, pp. 554-8.
Klein BS, Squires RA, Lloyd JK, et al. Canine antibody response to Blastomyces dermatitidis WI-1 antigen. Am J Vet Res. 2000;61(5):554-8.
Klein, B. S., Squires, R. A., Lloyd, J. K., Ruge, D. R., & Legendre, A. M. (2000). Canine antibody response to Blastomyces dermatitidis WI-1 antigen. American Journal of Veterinary Research, 61(5), 554-8.
Klein BS, et al. Canine Antibody Response to Blastomyces Dermatitidis WI-1 Antigen. Am J Vet Res. 2000;61(5):554-8. PubMed PMID: 10803652.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Canine antibody response to Blastomyces dermatitidis WI-1 antigen. AU - Klein,B S, AU - Squires,R A, AU - Lloyd,J K, AU - Ruge,D R, AU - Legendre,A M, PY - 2000/5/10/pubmed PY - 2000/7/8/medline PY - 2000/5/10/entrez SP - 554 EP - 8 JF - American journal of veterinary research JO - Am J Vet Res VL - 61 IS - 5 N2 - OBJECTIVE: To assess whether dogs with blastomycosis produce antibodies against the WI-1 and A-antigens of Blastomyces dermatitidis and whether the antibodies are useful in serodiagnosis. SAMPLE POPULATION: 359 serum samples obtained from 245 dogs. PROCEDURE: 233 samples from 122 dogs with blastomycosis, and 1 sample each from 24 dogs with suspected blastomycosis, 51 control dogs without infection, and 48 healthy dogs from an enzootic region were obtained. Antibodies against WI-1 antigen were detected by radioimmunoassay (RIA). Serum samples were tested in parallel for antibodies against the A-antigen of B dermatitidis by commercial agar-gel immunodiffusion (AGID) in a reference laboratory. RESULTS: Antibodies were detected in 92% of infected dogs by RIA and in 41 % by AGID. For 29 serum samples that were obtained 11 to 1,545 days after diagnosis, antibodies were detected in 92% of samples by RIA and 7% by AGID. For 93 serial serum samples from 29 dogs with blastomycosis, the mean anti-WI-1 titer was 1:18,761 at the time of diagnosis, and decreased to a mean of 1:1,338 by 210 days after treatment was initiated. Of 24 dogs with suspected infection, antibodies were detected in 67% by RIA and 33% by AGID. Control dogs without blastomycosis had no detectable antibodies in either assay. Thus, sensitivity was 92% for RIA and 41 % for AGID, and specificity was 100% for both tests. CONCLUSIONS AND CLINICAL RELEVANCE: Anti-WI-1 antibodies are readily detected by RIA in dogs with blastomycosis. Titers become high, decline during treatment, and persist for months. Anti-A antibodies are sometimes detected with AGID, but these decrease quickly. SN - 0002-9645 UR - https://www.unboundmedicine.com/medline/citation/10803652/Canine_antibody_response_to_Blastomyces_dermatitidis_WI_1_antigen_ L2 - https://avmajournals.avma.org/doi/10.2460/ajvr.2000.61.554?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -