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The contribution of endotoxins present in the respiratory tract to overproduction of nitric oxide associated with impaired interleukin 6 release in bronchoalveolar leukocytes from lung cancer patients.
Arch Immunol Ther Exp (Warsz). 2000; 48(2):119-25.AI

Abstract

The purpose of the study was to assess the relation between the levels of endotoxins circulating in airways of patients with lung cancer and the ability of bronchoalveolar lavage (BAL) leukocytes for ex vivo release of nitric oxide (NO) and interleukin 6 (IL-6) and for in vitro lipopolysaccharide (LPS)-induced production of the mediators. Leukocytes isolated from the BAL of 11 patients and from 5 healthy individuals were cultured in the absence or presence of LPS(E. coli). The levels of endotoxins in the BAL fluids (BALF) and the amounts of NO released ex vivo from unstimulated cells from the patients were highly (p = 0.0025) elevated in comparison with those from healthy individuals. The release of NO was significantly correlated (R(S) = 0.638, p = 0.047) with the levels of endotoxins in BALF. In contrast, production of IL-6 remained very low and a negative correlation (R(S) = -0.623, p = 0.0542) was observed between the amounts of NO and IL-6. It was also found that, in response to LPS, bronchoalveolar leukocytes from patients with lung cancer express a reduced capacity for in vitro production of NO and IL-6. Our data suggest that, in patients with lung cancer, the activation of BAL cells by endotoxins circulating in the airways may contribute, at least in part, to overproduction of spontaneous NO and, subsequently, the NO may reduce IL-6 production. Moreover, the exposure in vivo of the BAL cells to LPS renders them unable to respond to the second signals.

Authors+Show Affiliations

Laboratory of Virology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław. nowak@immuno.iitd.pan.wroc.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10807053

Citation

Cembrzyńska-Nowak, M, et al. "The Contribution of Endotoxins Present in the Respiratory Tract to Overproduction of Nitric Oxide Associated With Impaired Interleukin 6 Release in Bronchoalveolar Leukocytes From Lung Cancer Patients." Archivum Immunologiae Et Therapiae Experimentalis, vol. 48, no. 2, 2000, pp. 119-25.
Cembrzyńska-Nowak M, Bieńkowska M, Weryńska B, et al. The contribution of endotoxins present in the respiratory tract to overproduction of nitric oxide associated with impaired interleukin 6 release in bronchoalveolar leukocytes from lung cancer patients. Arch Immunol Ther Exp (Warsz). 2000;48(2):119-25.
Cembrzyńska-Nowak, M., Bieńkowska, M., Weryńska, B., Dyła, T., & Jankowska, R. (2000). The contribution of endotoxins present in the respiratory tract to overproduction of nitric oxide associated with impaired interleukin 6 release in bronchoalveolar leukocytes from lung cancer patients. Archivum Immunologiae Et Therapiae Experimentalis, 48(2), 119-25.
Cembrzyńska-Nowak M, et al. The Contribution of Endotoxins Present in the Respiratory Tract to Overproduction of Nitric Oxide Associated With Impaired Interleukin 6 Release in Bronchoalveolar Leukocytes From Lung Cancer Patients. Arch Immunol Ther Exp (Warsz). 2000;48(2):119-25. PubMed PMID: 10807053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The contribution of endotoxins present in the respiratory tract to overproduction of nitric oxide associated with impaired interleukin 6 release in bronchoalveolar leukocytes from lung cancer patients. AU - Cembrzyńska-Nowak,M, AU - Bieńkowska,M, AU - Weryńska,B, AU - Dyła,T, AU - Jankowska,R, PY - 2000/5/12/pubmed PY - 2000/7/25/medline PY - 2000/5/12/entrez SP - 119 EP - 25 JF - Archivum immunologiae et therapiae experimentalis JO - Arch Immunol Ther Exp (Warsz) VL - 48 IS - 2 N2 - The purpose of the study was to assess the relation between the levels of endotoxins circulating in airways of patients with lung cancer and the ability of bronchoalveolar lavage (BAL) leukocytes for ex vivo release of nitric oxide (NO) and interleukin 6 (IL-6) and for in vitro lipopolysaccharide (LPS)-induced production of the mediators. Leukocytes isolated from the BAL of 11 patients and from 5 healthy individuals were cultured in the absence or presence of LPS(E. coli). The levels of endotoxins in the BAL fluids (BALF) and the amounts of NO released ex vivo from unstimulated cells from the patients were highly (p = 0.0025) elevated in comparison with those from healthy individuals. The release of NO was significantly correlated (R(S) = 0.638, p = 0.047) with the levels of endotoxins in BALF. In contrast, production of IL-6 remained very low and a negative correlation (R(S) = -0.623, p = 0.0542) was observed between the amounts of NO and IL-6. It was also found that, in response to LPS, bronchoalveolar leukocytes from patients with lung cancer express a reduced capacity for in vitro production of NO and IL-6. Our data suggest that, in patients with lung cancer, the activation of BAL cells by endotoxins circulating in the airways may contribute, at least in part, to overproduction of spontaneous NO and, subsequently, the NO may reduce IL-6 production. Moreover, the exposure in vivo of the BAL cells to LPS renders them unable to respond to the second signals. SN - 0004-069X UR - https://www.unboundmedicine.com/medline/citation/10807053/The_contribution_of_endotoxins_present_in_the_respiratory_tract_to_overproduction_of_nitric_oxide_associated_with_impaired_interleukin_6_release_in_bronchoalveolar_leukocytes_from_lung_cancer_patients_ L2 - http://www.diseaseinfosearch.org/result/4334 DB - PRIME DP - Unbound Medicine ER -