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Relation between RLP-triglyceride to RLP-cholesterol ratio and particle size distribution in RLP-cholesterol profiles by HPLC.
Clin Chim Acta. 2000 Jun; 296(1-2):135-49.CC

Abstract

Remnant-Like Particles (RLP) isolated by an immunoseparation method are heterogeneous in their physical and biochemical properties. The objective of this study was to examine the relation between RLP-triglyceride (RLP-TG) to RLP-cholesterol (RLP-C) ratio and particle size distribution in RLP-C profiles from patients with hyperlipoproteinemia by HPLC. RLP were isolated from serum samples from 147 subjects. RLP-C and RLP-TG were quantified by respective enzymatic methods. Particle sizes of the RLP were measured using HPLC with 4 connected TSKgel LipopropakXL columns. Based on HPLC profiles of RLP-C from individual subjects, three different types were classified: predominantly LDL, predominantly VLDL, and mostly VLDL types. All patients with type III hyperlipidemia were mostly VLDL type but with smaller particle size of VLDL (32 nm) than other subjects. Severe hypertriglyceridemic (TG>4.52 mmoll(-1)) subjects were mostly VLDL type with large particle size (41 nm). As for all subjects (n=105) without predominantly LDL type, a significant correlation between RLP particle size and RLP-TG to RLP-C ratio (r=0. 432, P<0.001) was obtained, but not in case of serum TG to RLP-C ratio (r=0.062). It suggests that RLP-TG to RLP-C ratio might be used for discrimination of atherogenic smaller-sized lipoprotein from larger-sized TG-rich lipoprotein remnants.

Authors+Show Affiliations

Laboratory of Chemistry, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, 2-8-30, Kohnodai, Ichikawa-shi, Chiba, Japan. okazaki.cul@cul.tmd.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10807977

Citation

Okazaki, M, et al. "Relation Between RLP-triglyceride to RLP-cholesterol Ratio and Particle Size Distribution in RLP-cholesterol Profiles By HPLC." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 296, no. 1-2, 2000, pp. 135-49.
Okazaki M, Usui S, Tada N, et al. Relation between RLP-triglyceride to RLP-cholesterol ratio and particle size distribution in RLP-cholesterol profiles by HPLC. Clin Chim Acta. 2000;296(1-2):135-49.
Okazaki, M., Usui, S., Tada, N., Nakano, T., & Nakajima, K. (2000). Relation between RLP-triglyceride to RLP-cholesterol ratio and particle size distribution in RLP-cholesterol profiles by HPLC. Clinica Chimica Acta; International Journal of Clinical Chemistry, 296(1-2), 135-49.
Okazaki M, et al. Relation Between RLP-triglyceride to RLP-cholesterol Ratio and Particle Size Distribution in RLP-cholesterol Profiles By HPLC. Clin Chim Acta. 2000;296(1-2):135-49. PubMed PMID: 10807977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relation between RLP-triglyceride to RLP-cholesterol ratio and particle size distribution in RLP-cholesterol profiles by HPLC. AU - Okazaki,M, AU - Usui,S, AU - Tada,N, AU - Nakano,T, AU - Nakajima,K, PY - 2000/5/16/pubmed PY - 2000/7/25/medline PY - 2000/5/16/entrez SP - 135 EP - 49 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin Chim Acta VL - 296 IS - 1-2 N2 - Remnant-Like Particles (RLP) isolated by an immunoseparation method are heterogeneous in their physical and biochemical properties. The objective of this study was to examine the relation between RLP-triglyceride (RLP-TG) to RLP-cholesterol (RLP-C) ratio and particle size distribution in RLP-C profiles from patients with hyperlipoproteinemia by HPLC. RLP were isolated from serum samples from 147 subjects. RLP-C and RLP-TG were quantified by respective enzymatic methods. Particle sizes of the RLP were measured using HPLC with 4 connected TSKgel LipopropakXL columns. Based on HPLC profiles of RLP-C from individual subjects, three different types were classified: predominantly LDL, predominantly VLDL, and mostly VLDL types. All patients with type III hyperlipidemia were mostly VLDL type but with smaller particle size of VLDL (32 nm) than other subjects. Severe hypertriglyceridemic (TG>4.52 mmoll(-1)) subjects were mostly VLDL type with large particle size (41 nm). As for all subjects (n=105) without predominantly LDL type, a significant correlation between RLP particle size and RLP-TG to RLP-C ratio (r=0. 432, P<0.001) was obtained, but not in case of serum TG to RLP-C ratio (r=0.062). It suggests that RLP-TG to RLP-C ratio might be used for discrimination of atherogenic smaller-sized lipoprotein from larger-sized TG-rich lipoprotein remnants. SN - 0009-8981 UR - https://www.unboundmedicine.com/medline/citation/10807977/Relation_between_RLP_triglyceride_to_RLP_cholesterol_ratio_and_particle_size_distribution_in_RLP_cholesterol_profiles_by_HPLC_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009898100002138 DB - PRIME DP - Unbound Medicine ER -