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Hfq (HF1) stimulates ompA mRNA decay by interfering with ribosome binding.
Genes Dev. 2000 May 01; 14(9):1109-18.GD

Abstract

The adaptation of mRNA stability to environmental changes is a means of cells to adjust the level of gene expression. The Escherichia coli ompA mRNA has served as one of the paradigms for regulated mRNA decay in prokaryotes. The stability of the transcript is known to be correlated inversely with the bacterial growth rate. Thus, the regulation of ompA mRNA stability meets the physiological needs to adjust the level of ompA expression to the rate of cell division. Recently, host factor I (Hfq/HF1) was shown to be involved in the regulation of ompA mRNA stability under slow growth conditions. Here, we present the first direct demonstration that 30S ribosomes bound to the ompA 5'-UTR protect the transcript from RNase E cleavage in vitro. However, the 30S protection was found to be abrogated in the presence of Hfq. Toeprinting and in vitro translation assays revealed that translation of ompA is repressed in the presence of Hfq. These in vitro studies are corroborated by in vivo expression studies demonstrating that the reduced synthesis rate of OmpA effected by Hfq results in functional inactivation of the ompA mRNA. The data are discussed in terms of a model wherein Hfq regulates the stability of ompA mRNA by competing with 30S ribosomes for binding to the ompA 5'-UTR.

Authors+Show Affiliations

Institute of Microbiology and Genetics, Vienna Biocenter, 1030 Vienna, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10809669

Citation

Vytvytska, O, et al. "Hfq (HF1) Stimulates ompA mRNA Decay By Interfering With Ribosome Binding." Genes & Development, vol. 14, no. 9, 2000, pp. 1109-18.
Vytvytska O, Moll I, Kaberdin VR, et al. Hfq (HF1) stimulates ompA mRNA decay by interfering with ribosome binding. Genes Dev. 2000;14(9):1109-18.
Vytvytska, O., Moll, I., Kaberdin, V. R., von Gabain, A., & Bläsi, U. (2000). Hfq (HF1) stimulates ompA mRNA decay by interfering with ribosome binding. Genes & Development, 14(9), 1109-18.
Vytvytska O, et al. Hfq (HF1) Stimulates ompA mRNA Decay By Interfering With Ribosome Binding. Genes Dev. 2000 May 1;14(9):1109-18. PubMed PMID: 10809669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hfq (HF1) stimulates ompA mRNA decay by interfering with ribosome binding. AU - Vytvytska,O, AU - Moll,I, AU - Kaberdin,V R, AU - von Gabain,A, AU - Bläsi,U, PY - 2000/5/16/pubmed PY - 2000/6/24/medline PY - 2000/5/16/entrez SP - 1109 EP - 18 JF - Genes & development JO - Genes Dev VL - 14 IS - 9 N2 - The adaptation of mRNA stability to environmental changes is a means of cells to adjust the level of gene expression. The Escherichia coli ompA mRNA has served as one of the paradigms for regulated mRNA decay in prokaryotes. The stability of the transcript is known to be correlated inversely with the bacterial growth rate. Thus, the regulation of ompA mRNA stability meets the physiological needs to adjust the level of ompA expression to the rate of cell division. Recently, host factor I (Hfq/HF1) was shown to be involved in the regulation of ompA mRNA stability under slow growth conditions. Here, we present the first direct demonstration that 30S ribosomes bound to the ompA 5'-UTR protect the transcript from RNase E cleavage in vitro. However, the 30S protection was found to be abrogated in the presence of Hfq. Toeprinting and in vitro translation assays revealed that translation of ompA is repressed in the presence of Hfq. These in vitro studies are corroborated by in vivo expression studies demonstrating that the reduced synthesis rate of OmpA effected by Hfq results in functional inactivation of the ompA mRNA. The data are discussed in terms of a model wherein Hfq regulates the stability of ompA mRNA by competing with 30S ribosomes for binding to the ompA 5'-UTR. SN - 0890-9369 UR - https://www.unboundmedicine.com/medline/citation/10809669/Hfq__HF1__stimulates_ompA_mRNA_decay_by_interfering_with_ribosome_binding_ L2 - http://www.genesdev.org/cgi/pmidlookup?view=long&pmid=10809669 DB - PRIME DP - Unbound Medicine ER -