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Study of major flavonoids in crude Scutellariae Radix by micellar electrokinetic capillary chromatography.
J Capillary Electrophor. 1998 Sep-Dec; 5(5-6):181-4.JC

Abstract

A method of micellar electrokinetic capillary electrophoresis for determining the six flavonoids in Scutellariae Radix (i.e., baicalin, baicalein, wogonin 7-O-glucuronide, wogonin, oroxylin A 7-O-glucuronide, and oroxylin A) has been developed. The buffer solution (pH 7.24) composed of 20 mM sodium dodecyl sulfate (SDS), 20 mM sodium dihydrogen phosphate, and 25 mM sodium borate was found to be the most suitable electrolyte for the separation. The contents of the six flavonoids in crude Scutellariae Radix could easily be determined within about 15 min. On-column UV (254 nm) monitoring allowed the quantitative determination of baicalin. The effects of pH, surfactant concentration, and applied voltage on the migration behavior of the solutes were studied.

Authors+Show Affiliations

Qi L
Beijing New Technology Application Institute, P.R. China.
Zhou R
No affiliation info available
Wang YF
No affiliation info available
Zhu YC
No affiliation info available

MeSH

ChinaDrugs, Chinese HerbalElectrophoresis, CapillaryFlavonoidsMicellesPlant ExtractsPlants, Medicinal

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10812426

Citation

Qi, L, et al. "Study of Major Flavonoids in Crude Scutellariae Radix By Micellar Electrokinetic Capillary Chromatography." Journal of Capillary Electrophoresis, vol. 5, no. 5-6, 1998, pp. 181-4.
Qi L, Zhou R, Wang YF, et al. Study of major flavonoids in crude Scutellariae Radix by micellar electrokinetic capillary chromatography. J Capillary Electrophor. 1998;5(5-6):181-4.
Qi, L., Zhou, R., Wang, Y. F., & Zhu, Y. C. (1998). Study of major flavonoids in crude Scutellariae Radix by micellar electrokinetic capillary chromatography. Journal of Capillary Electrophoresis, 5(5-6), 181-4.
Qi L, et al. Study of Major Flavonoids in Crude Scutellariae Radix By Micellar Electrokinetic Capillary Chromatography. J Capillary Electrophor. 1998 Sep-Dec;5(5-6):181-4. PubMed PMID: 10812426.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Study of major flavonoids in crude Scutellariae Radix by micellar electrokinetic capillary chromatography. AU - Qi,L, AU - Zhou,R, AU - Wang,Y F, AU - Zhu,Y C, PY - 2000/5/17/pubmed PY - 2000/6/10/medline PY - 2000/5/17/entrez SP - 181 EP - 4 JF - Journal of capillary electrophoresis JO - J Capillary Electrophor VL - 5 IS - 5-6 N2 - A method of micellar electrokinetic capillary electrophoresis for determining the six flavonoids in Scutellariae Radix (i.e., baicalin, baicalein, wogonin 7-O-glucuronide, wogonin, oroxylin A 7-O-glucuronide, and oroxylin A) has been developed. The buffer solution (pH 7.24) composed of 20 mM sodium dodecyl sulfate (SDS), 20 mM sodium dihydrogen phosphate, and 25 mM sodium borate was found to be the most suitable electrolyte for the separation. The contents of the six flavonoids in crude Scutellariae Radix could easily be determined within about 15 min. On-column UV (254 nm) monitoring allowed the quantitative determination of baicalin. The effects of pH, surfactant concentration, and applied voltage on the migration behavior of the solutes were studied. SN - 1079-5383 UR - https://www.unboundmedicine.com/medline/citation/10812426/Study_of_major_flavonoids_in_crude_Scutellariae_Radix_by_micellar_electrokinetic_capillary_chromatography_ DB - PRIME DP - Unbound Medicine ER -