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Epidermal growth factor alters the bax:bcl-w ratio following massive small bowel resection.
J Surg Res. 2000 Jun 01; 91(1):38-42.JS

Abstract

BACKGROUND

Following massive small bowel resection (SBR), the expression of bax and bcl-w is associated with increased enterocyte apoptosis. Epidermal growth factor (EGF) has been shown to enhance enterocyte proliferation and retard apoptosis in the adapting bowel. This study examined the effect of EGF on the expression of these bcl-2 family members during adaptation.

MATERIALS AND METHODS

Mice (C57Bl/6; n = 38) underwent a 50% SBR or sham operation and then were randomized to receive twice-daily orogastric saline or EGF (50 microg/kg/day). After 3 days, the remnant ileum was removed, apoptotic index (No. apoptotic bodies/crypt) calculated, and expression of mRNA and protein for bax and bcl-w quantified.

RESULTS

EGF prevented the expected increase in the apoptotic index after SBR and altered the ratio of bax to bcl-w in favor of cell survival.

CONCLUSION

Following massive small bowel resection, EGF retards rates of enterocyte apoptosis and modifies the expression of bcl-2 family members. By decreasing bax and increasing bcl-w expression, the balance between pro- and anti-apoptotic genes is shifted in favor of cell survival. Alteration of bcl-2 family member expression may be an important mechanism by which EGF reduces the increased enterocyte apoptosis that occurs after massive small bowel resection.

Authors+Show Affiliations

Division of Pediatric Surgery, Children's Hospital Medical Center, Cincinnati, Ohio 45229-3039, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10816347

Citation

Stern, L E., et al. "Epidermal Growth Factor Alters the Bax:bcl-w Ratio Following Massive Small Bowel Resection." The Journal of Surgical Research, vol. 91, no. 1, 2000, pp. 38-42.
Stern LE, Falcone RA, Huang F, et al. Epidermal growth factor alters the bax:bcl-w ratio following massive small bowel resection. J Surg Res. 2000;91(1):38-42.
Stern, L. E., Falcone, R. A., Huang, F., Kemp, C. J., Erwin, C. R., & Warner, B. W. (2000). Epidermal growth factor alters the bax:bcl-w ratio following massive small bowel resection. The Journal of Surgical Research, 91(1), 38-42.
Stern LE, et al. Epidermal Growth Factor Alters the Bax:bcl-w Ratio Following Massive Small Bowel Resection. J Surg Res. 2000 Jun 1;91(1):38-42. PubMed PMID: 10816347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epidermal growth factor alters the bax:bcl-w ratio following massive small bowel resection. AU - Stern,L E, AU - Falcone,R A,Jr AU - Huang,F, AU - Kemp,C J, AU - Erwin,C R, AU - Warner,B W, PY - 2000/5/19/pubmed PY - 2000/6/17/medline PY - 2000/5/19/entrez SP - 38 EP - 42 JF - The Journal of surgical research JO - J Surg Res VL - 91 IS - 1 N2 - BACKGROUND: Following massive small bowel resection (SBR), the expression of bax and bcl-w is associated with increased enterocyte apoptosis. Epidermal growth factor (EGF) has been shown to enhance enterocyte proliferation and retard apoptosis in the adapting bowel. This study examined the effect of EGF on the expression of these bcl-2 family members during adaptation. MATERIALS AND METHODS: Mice (C57Bl/6; n = 38) underwent a 50% SBR or sham operation and then were randomized to receive twice-daily orogastric saline or EGF (50 microg/kg/day). After 3 days, the remnant ileum was removed, apoptotic index (No. apoptotic bodies/crypt) calculated, and expression of mRNA and protein for bax and bcl-w quantified. RESULTS: EGF prevented the expected increase in the apoptotic index after SBR and altered the ratio of bax to bcl-w in favor of cell survival. CONCLUSION: Following massive small bowel resection, EGF retards rates of enterocyte apoptosis and modifies the expression of bcl-2 family members. By decreasing bax and increasing bcl-w expression, the balance between pro- and anti-apoptotic genes is shifted in favor of cell survival. Alteration of bcl-2 family member expression may be an important mechanism by which EGF reduces the increased enterocyte apoptosis that occurs after massive small bowel resection. SN - 0022-4804 UR - https://www.unboundmedicine.com/medline/citation/10816347/Epidermal_growth_factor_alters_the_bax:bcl_w_ratio_following_massive_small_bowel_resection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(00)95897-9 DB - PRIME DP - Unbound Medicine ER -