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Use of a plasmid of a yersinia enterocolitica biogroup 1A strain for the construction of cloning vectors.
J Biotechnol 2000; 79(1):63-72JB

Abstract

A plasmid with a size of 2,682 base pairs isolated from the Yersinia enterocolitica biogroup 1A strain # 29807 was characterized in respect to its suitability as a basic replicon for cloning vectors. The copy number of the plasmid was determined to be approximately 14 copies per cell and it was shown to be compatible with vectors with an origin of replication derived from ColE1 and p115A. The replication region of the plasmid encodes a primer RNAI and countertranscript RNAII. Two vectors, pIV1 and pIV2, containing a kanamycin resistance gene and the lacZalpha fragment with the multiple cloning site of pBluescriptSK + were constructed. A mobilizable derivative was successfully introduced into different bacteria belonging to the family Enterobacteriacea. To prove the applicability of the novel vectors for cloning purposes, a 13 kb hemolysin operon of Escherichia coli was inserted into pIV1, and the resulting recombinant plasmid was stably maintained and expressed in E. coli and Y. enterocolitica.

Authors+Show Affiliations

Projektgruppe Biologische Sicherheit, Robert Koch-lnstitut, Berlin, Germany. strauche@rki.deNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10817342

Citation

Strauch, E, et al. "Use of a Plasmid of a Yersinia Enterocolitica Biogroup 1A Strain for the Construction of Cloning Vectors." Journal of Biotechnology, vol. 79, no. 1, 2000, pp. 63-72.
Strauch E, Voigt I, Broll H, et al. Use of a plasmid of a yersinia enterocolitica biogroup 1A strain for the construction of cloning vectors. J Biotechnol. 2000;79(1):63-72.
Strauch, E., Voigt, I., Broll, H., & Appel, B. (2000). Use of a plasmid of a yersinia enterocolitica biogroup 1A strain for the construction of cloning vectors. Journal of Biotechnology, 79(1), pp. 63-72.
Strauch E, et al. Use of a Plasmid of a Yersinia Enterocolitica Biogroup 1A Strain for the Construction of Cloning Vectors. J Biotechnol. 2000 Apr 14;79(1):63-72. PubMed PMID: 10817342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of a plasmid of a yersinia enterocolitica biogroup 1A strain for the construction of cloning vectors. AU - Strauch,E, AU - Voigt,I, AU - Broll,H, AU - Appel,B, PY - 2000/5/19/pubmed PY - 2000/7/8/medline PY - 2000/5/19/entrez SP - 63 EP - 72 JF - Journal of biotechnology JO - J. Biotechnol. VL - 79 IS - 1 N2 - A plasmid with a size of 2,682 base pairs isolated from the Yersinia enterocolitica biogroup 1A strain # 29807 was characterized in respect to its suitability as a basic replicon for cloning vectors. The copy number of the plasmid was determined to be approximately 14 copies per cell and it was shown to be compatible with vectors with an origin of replication derived from ColE1 and p115A. The replication region of the plasmid encodes a primer RNAI and countertranscript RNAII. Two vectors, pIV1 and pIV2, containing a kanamycin resistance gene and the lacZalpha fragment with the multiple cloning site of pBluescriptSK + were constructed. A mobilizable derivative was successfully introduced into different bacteria belonging to the family Enterobacteriacea. To prove the applicability of the novel vectors for cloning purposes, a 13 kb hemolysin operon of Escherichia coli was inserted into pIV1, and the resulting recombinant plasmid was stably maintained and expressed in E. coli and Y. enterocolitica. SN - 0168-1656 UR - https://www.unboundmedicine.com/medline/citation/10817342/Use_of_a_plasmid_of_a_yersinia_enterocolitica_biogroup_1A_strain_for_the_construction_of_cloning_vectors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1656(00)00216-9 DB - PRIME DP - Unbound Medicine ER -