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Quantitative assay of metronidazole by capillary zone electrophoresis with amperometric detection at a gold microelectrode.
Electrophoresis. 2000 Apr; 21(7):1409-14.E

Abstract

Capillary zone electrophoresis was employed for the determination of metronidazole using end-column amperometric detection with a gold microelectrode at a constant potential of -0.52V vs. saturated calomel electrode. To overcome interference of oxygen in the solution, a deaeration injector and a deaeration protector at the detection cell were used. The optimum conditions of separation and detection are 1.0 x 10(-3) mol/L potassium dihydrogen citrate (KH2C6H5O7) for the buffer solution, 20 kV for the separation voltage, and 5 kV and 10 S for injection voltage and injection time, respectively. The limit of detection is 6.0 x 10(7) mol/L or 0.78 fmole (S/N = 3). The relative standard deviation is 3.9% for the electrophoretic peak current. The method was applied to the determination of metronidazole in human urine.

Authors+Show Affiliations

Jin W
Laboratoty of Analytical Science, School of Chemistry, Shandong University, Jinan, China. wenrujin@jn-public.sd.cninfo.net
Li W
No affiliation info available
Xu Q
No affiliation info available
Dong Q
No affiliation info available

MeSH

Electrophoresis, CapillaryGoldHumansMetronidazoleMicroelectrodesMolecular Structure

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10826688

Citation

Jin, W, et al. "Quantitative Assay of Metronidazole By Capillary Zone Electrophoresis With Amperometric Detection at a Gold Microelectrode." Electrophoresis, vol. 21, no. 7, 2000, pp. 1409-14.
Jin W, Li W, Xu Q, et al. Quantitative assay of metronidazole by capillary zone electrophoresis with amperometric detection at a gold microelectrode. Electrophoresis. 2000;21(7):1409-14.
Jin, W., Li, W., Xu, Q., & Dong, Q. (2000). Quantitative assay of metronidazole by capillary zone electrophoresis with amperometric detection at a gold microelectrode. Electrophoresis, 21(7), 1409-14.
Jin W, et al. Quantitative Assay of Metronidazole By Capillary Zone Electrophoresis With Amperometric Detection at a Gold Microelectrode. Electrophoresis. 2000;21(7):1409-14. PubMed PMID: 10826688.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative assay of metronidazole by capillary zone electrophoresis with amperometric detection at a gold microelectrode. AU - Jin,W, AU - Li,W, AU - Xu,Q, AU - Dong,Q, PY - 2000/5/29/pubmed PY - 2000/9/23/medline PY - 2000/5/29/entrez SP - 1409 EP - 14 JF - Electrophoresis JO - Electrophoresis VL - 21 IS - 7 N2 - Capillary zone electrophoresis was employed for the determination of metronidazole using end-column amperometric detection with a gold microelectrode at a constant potential of -0.52V vs. saturated calomel electrode. To overcome interference of oxygen in the solution, a deaeration injector and a deaeration protector at the detection cell were used. The optimum conditions of separation and detection are 1.0 x 10(-3) mol/L potassium dihydrogen citrate (KH2C6H5O7) for the buffer solution, 20 kV for the separation voltage, and 5 kV and 10 S for injection voltage and injection time, respectively. The limit of detection is 6.0 x 10(7) mol/L or 0.78 fmole (S/N = 3). The relative standard deviation is 3.9% for the electrophoretic peak current. The method was applied to the determination of metronidazole in human urine. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/10826688/Quantitative_assay_of_metronidazole_by_capillary_zone_electrophoresis_with_amperometric_detection_at_a_gold_microelectrode_ L2 - https://doi.org/10.1002/(SICI)1522-2683(20000401)21:7<1409::AID-ELPS1409>3.0.CO;2-0 DB - PRIME DP - Unbound Medicine ER -