TY - JOUR T1 - Determination of clozapine by capillary zone electrophoresis following end-column amperometric detection with simplified capillary/electrode alignment. AU - Jin,W, AU - Xu,Q, AU - Li,W, PY - 2000/5/29/pubmed PY - 2000/9/23/medline PY - 2000/5/29/entrez SP - 1415 EP - 20 JF - Electrophoresis JO - Electrophoresis VL - 21 IS - 7 N2 - Capillary zone electrophoresis was employed for the determination of clozapine using an end-column amperometric detection at a carbon fiber array microdisk electrode with simplified capillary/electrode alignment. The optimum conditions of separation and detection are: Britton-Robinson buffer, pH 2.0 (1.3 x 10(-2) mol/L total concentration of acids, 3.2 x 10(-3) mol/L NaOH), 15 kV for separation voltage, 5 kV and 10 s for injection voltage and injection time, respectively. The limit of detection is 4.2 x 10(-7) mol/L or 1.2 fmole (signal to noise, S/N = 2). The relative standard deviation is 1.4% for the migration time and 2.5% for the electrophoretic peak current. The method was applied to the determination of clozapine in human blood. The recovery of the method is between 94-104%. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/10826689/Determination_of_clozapine_by_capillary_zone_electrophoresis_following_end_column_amperometric_detection_with_simplified_capillary/electrode_alignment_ L2 - https://doi.org/10.1002/(SICI)1522-2683(20000401)21:7<1415::AID-ELPS1415>3.0.CO;2-M DB - PRIME DP - Unbound Medicine ER -