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HPLC determination of catechins and caffeine in tea. Differentiation of green, black and instant teas.
Analyst. 2000 Mar; 125(3):421-5.A

Abstract

A simple and fast high performance liquid chromatographic method for five catechins and caffeine using an ODS column and a water-acetonitrile-formic acid mobile phase system was developed. The catechins (epicatechin, catechin, epigallocatechin, epigallocatechin gallate, epicatechin gallate) and caffeine were separated by an acetonitrile gradient within 20 min. The detection limit of the method was approximately 10 ng for all the compounds (by injecting 10 microL). Several green, black and instant teas were analysed using this method. By using the studied compounds as chemical descriptors, linear discriminant analysis was performed and complete differentiation of the green, black and instant teas was achieved.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Chemistry, University of Seville, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10829341

Citation

Fernández, P L., et al. "HPLC Determination of Catechins and Caffeine in Tea. Differentiation of Green, Black and Instant Teas." The Analyst, vol. 125, no. 3, 2000, pp. 421-5.
Fernández PL, Martín MJ, González AG, et al. HPLC determination of catechins and caffeine in tea. Differentiation of green, black and instant teas. Analyst. 2000;125(3):421-5.
Fernández, P. L., Martín, M. J., González, A. G., & Pablos, F. (2000). HPLC determination of catechins and caffeine in tea. Differentiation of green, black and instant teas. The Analyst, 125(3), 421-5.
Fernández PL, et al. HPLC Determination of Catechins and Caffeine in Tea. Differentiation of Green, Black and Instant Teas. Analyst. 2000;125(3):421-5. PubMed PMID: 10829341.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPLC determination of catechins and caffeine in tea. Differentiation of green, black and instant teas. AU - Fernández,P L, AU - Martín,M J, AU - González,A G, AU - Pablos,F, PY - 2000/6/1/pubmed PY - 2000/6/17/medline PY - 2000/6/1/entrez SP - 421 EP - 5 JF - The Analyst JO - Analyst VL - 125 IS - 3 N2 - A simple and fast high performance liquid chromatographic method for five catechins and caffeine using an ODS column and a water-acetonitrile-formic acid mobile phase system was developed. The catechins (epicatechin, catechin, epigallocatechin, epigallocatechin gallate, epicatechin gallate) and caffeine were separated by an acetonitrile gradient within 20 min. The detection limit of the method was approximately 10 ng for all the compounds (by injecting 10 microL). Several green, black and instant teas were analysed using this method. By using the studied compounds as chemical descriptors, linear discriminant analysis was performed and complete differentiation of the green, black and instant teas was achieved. SN - 0003-2654 UR - https://www.unboundmedicine.com/medline/citation/10829341/HPLC_determination_of_catechins_and_caffeine_in_tea__Differentiation_of_green_black_and_instant_teas_ L2 - https://doi.org/10.1039/a909219f DB - PRIME DP - Unbound Medicine ER -