Tags

Type your tag names separated by a space and hit enter

Antihyperglycaemic and anti-oxidant properties of Andrographis paniculata in normal and diabetic rats.
Clin Exp Pharmacol Physiol. 2000 May-Jun; 27(5-6):358-63.CE

Abstract

1. Oxidative stress is believed to be a pathogenetic factor in the development of diabetic complications. In the present study, we investigated the ethanolic extract of the aerial parts of Andrographis paniculata for antihyperglycaemic and anti-oxidant effects in normal and streptozotocin-induced type I diabetic rats. 2. Normal and diabetic rats were randomly divided into groups and treated orally by gavage with vehicle (distilled water), metformin (500 mg/kg bodyweight) or the extract (400 mg/kg bodyweight), twice a day for 14 days. 3. At the end of the 14 day period, the extract, like metformin, significantly increased bodyweight (P < 0.01) and reduced fasting serum glucose in diabetic rats (P < 0.001) when compared with vehicle, but had no effect on bodyweight and serum glucose in normal rats. Levels of liver and kidney thiobarbituric acid-reactive substances (TBARS) were significantly increased (P < 0.0001, P < 0.01, respectively), while liver glutathione (GSH) concentrations were significantly decreased (P < 0.005) in vehicle-treated diabetic rats. Liver and kidney TBARS levels were significantly lower (P < 0.0001, P < 0.005, respectively), whereas liver GSH concentrations were significantly higher (P < 0.05) in extract- and metformin-treated diabetic rats compared with vehicle-treated diabetic rats. Andrographis paniculata significantly decreased kidney TBARS level (P < 0.005) in normal rats. Hepatic superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities were significantly lower in vehicle-treated diabetic rats compared with vehicle-treated normal rats. The extract, as well as metformin, significantly increased the activity of SOD and CAT, but had no significant effect on GSH-Px activity in diabetic rats. The extract and metformin did not produce significant changes in the activity of these anti-oxidant enzymes in normal rats. 4. Our results show that oxidative stress is evident in streptozotocin-diabetic rats and indicate that the ethanolic extract of A. paniculata not only possesses an antihyperglycaemic property, but may also reduce oxidative stress in diabetic rats.

Links

Publisher Full Text
Aggregator Full Text

Authors+Show Affiliations

Zhang XF
Department of Pharmacology, Faculty of Medicine, National University of Singapore, Singapore.
Tan BK
No affiliation info available

MeSH

AnimalsAnti-Bacterial AgentsAntioxidantsBody WeightDiabetes Mellitus, ExperimentalFastingHypoglycemic AgentsKidneyLiverMaleMetforminOxidative StressPlant ExtractsRatsRats, Sprague-DawleyStreptozocin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10831236

Citation

Zhang, X F., and B K. Tan. "Antihyperglycaemic and Anti-oxidant Properties of Andrographis Paniculata in Normal and Diabetic Rats." Clinical and Experimental Pharmacology & Physiology, vol. 27, no. 5-6, 2000, pp. 358-63.
Zhang XF, Tan BK. Antihyperglycaemic and anti-oxidant properties of Andrographis paniculata in normal and diabetic rats. Clin Exp Pharmacol Physiol. 2000;27(5-6):358-63.
Zhang, X. F., & Tan, B. K. (2000). Antihyperglycaemic and anti-oxidant properties of Andrographis paniculata in normal and diabetic rats. Clinical and Experimental Pharmacology & Physiology, 27(5-6), 358-63.
Zhang XF, Tan BK. Antihyperglycaemic and Anti-oxidant Properties of Andrographis Paniculata in Normal and Diabetic Rats. Clin Exp Pharmacol Physiol. 2000 May-Jun;27(5-6):358-63. PubMed PMID: 10831236.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antihyperglycaemic and anti-oxidant properties of Andrographis paniculata in normal and diabetic rats. AU - Zhang,X F, AU - Tan,B K, PY - 2000/6/1/pubmed PY - 2000/9/23/medline PY - 2000/6/1/entrez SP - 358 EP - 63 JF - Clinical and experimental pharmacology & physiology JO - Clin Exp Pharmacol Physiol VL - 27 IS - 5-6 N2 - 1. Oxidative stress is believed to be a pathogenetic factor in the development of diabetic complications. In the present study, we investigated the ethanolic extract of the aerial parts of Andrographis paniculata for antihyperglycaemic and anti-oxidant effects in normal and streptozotocin-induced type I diabetic rats. 2. Normal and diabetic rats were randomly divided into groups and treated orally by gavage with vehicle (distilled water), metformin (500 mg/kg bodyweight) or the extract (400 mg/kg bodyweight), twice a day for 14 days. 3. At the end of the 14 day period, the extract, like metformin, significantly increased bodyweight (P < 0.01) and reduced fasting serum glucose in diabetic rats (P < 0.001) when compared with vehicle, but had no effect on bodyweight and serum glucose in normal rats. Levels of liver and kidney thiobarbituric acid-reactive substances (TBARS) were significantly increased (P < 0.0001, P < 0.01, respectively), while liver glutathione (GSH) concentrations were significantly decreased (P < 0.005) in vehicle-treated diabetic rats. Liver and kidney TBARS levels were significantly lower (P < 0.0001, P < 0.005, respectively), whereas liver GSH concentrations were significantly higher (P < 0.05) in extract- and metformin-treated diabetic rats compared with vehicle-treated diabetic rats. Andrographis paniculata significantly decreased kidney TBARS level (P < 0.005) in normal rats. Hepatic superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities were significantly lower in vehicle-treated diabetic rats compared with vehicle-treated normal rats. The extract, as well as metformin, significantly increased the activity of SOD and CAT, but had no significant effect on GSH-Px activity in diabetic rats. The extract and metformin did not produce significant changes in the activity of these anti-oxidant enzymes in normal rats. 4. Our results show that oxidative stress is evident in streptozotocin-diabetic rats and indicate that the ethanolic extract of A. paniculata not only possesses an antihyperglycaemic property, but may also reduce oxidative stress in diabetic rats. SN - 0305-1870 UR - https://www.unboundmedicine.com/medline/citation/10831236/Antihyperglycaemic_and_anti_oxidant_properties_of_Andrographis_paniculata_in_normal_and_diabetic_rats_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&amp;sid=nlm:pubmed&amp;issn=0305-1870&amp;date=2000&amp;volume=27&amp;issue=5-6&amp;spage=358 DB - PRIME DP - Unbound Medicine ER -