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Monitoring myoglobin by capillary zone electrophoresis with end-column amperometric detection.
Electrophoresis. 2000 May; 21(8):1535-9.E

Abstract

Capillary zone electrophoresis was employed for the determination of myoglobin in human urine using end-column amperometric detection with a carbon fiber microelectrode at a constant potential of 1.80 V vs. saturated calomel electrode (SCF). The optimum conditions of separation and detection are: 3.73 x 10-4 mol/L sodium diethyl malonyl urea (barbitone sodium), 1.34 x 10-4 mol/L HCl for the buffer solution, 20 kV for separation voltage, 5 kV and 5 s for injection voltage and injection time, respectively. The limit of detection is 4.4 x 10-8 mol/L or 84 amole signal to noise (S/N = 2). The relative standard deviation is 2.9% for the migration time and 2.5% for the electrophoretic peak current. The method can be used for the determination of myoglobin in human urine. The samples can be directly injected and need no pretreatment. The method is also rapid, less than 2 min, and has a recovery rate of 94-106%.

Authors+Show Affiliations

Jin W
Laboratory of Analytical Science, School of Chemistry, Shandong University, Jinan, China. wenrujin@jn-public.sd.cninfo.net
Dong Q
No affiliation info available
Yu D
No affiliation info available
Ye X
No affiliation info available

MeSH

Electrophoresis, CapillaryHumansMyoglobinuriaReproducibility of Results

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10832884

Citation

Jin, W, et al. "Monitoring Myoglobin By Capillary Zone Electrophoresis With End-column Amperometric Detection." Electrophoresis, vol. 21, no. 8, 2000, pp. 1535-9.
Jin W, Dong Q, Yu D, et al. Monitoring myoglobin by capillary zone electrophoresis with end-column amperometric detection. Electrophoresis. 2000;21(8):1535-9.
Jin, W., Dong, Q., Yu, D., & Ye, X. (2000). Monitoring myoglobin by capillary zone electrophoresis with end-column amperometric detection. Electrophoresis, 21(8), 1535-9.
Jin W, et al. Monitoring Myoglobin By Capillary Zone Electrophoresis With End-column Amperometric Detection. Electrophoresis. 2000;21(8):1535-9. PubMed PMID: 10832884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monitoring myoglobin by capillary zone electrophoresis with end-column amperometric detection. AU - Jin,W, AU - Dong,Q, AU - Yu,D, AU - Ye,X, PY - 2000/6/1/pubmed PY - 2000/9/30/medline PY - 2000/6/1/entrez SP - 1535 EP - 9 JF - Electrophoresis JO - Electrophoresis VL - 21 IS - 8 N2 - Capillary zone electrophoresis was employed for the determination of myoglobin in human urine using end-column amperometric detection with a carbon fiber microelectrode at a constant potential of 1.80 V vs. saturated calomel electrode (SCF). The optimum conditions of separation and detection are: 3.73 x 10-4 mol/L sodium diethyl malonyl urea (barbitone sodium), 1.34 x 10-4 mol/L HCl for the buffer solution, 20 kV for separation voltage, 5 kV and 5 s for injection voltage and injection time, respectively. The limit of detection is 4.4 x 10-8 mol/L or 84 amole signal to noise (S/N = 2). The relative standard deviation is 2.9% for the migration time and 2.5% for the electrophoretic peak current. The method can be used for the determination of myoglobin in human urine. The samples can be directly injected and need no pretreatment. The method is also rapid, less than 2 min, and has a recovery rate of 94-106%. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/10832884/Monitoring_myoglobin_by_capillary_zone_electrophoresis_with_end_column_amperometric_detection_ L2 - https://doi.org/10.1002/(SICI)1522-2683(20000501)21:8<1535::AID-ELPS1535>3.0.CO;2-8 DB - PRIME DP - Unbound Medicine ER -