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Telomerase activity in pure pancreatic juice for the diagnosis of pancreatic cancer may be complementary to K-ras mutation.
Gastrointest Endosc. 2000 Jun; 51(6):708-13.GE

Abstract

BACKGROUND

The usefulness of K-ras mutation in pancreatic juice for the diagnosis of pancreatic cancer is questionable. Telomerase is positive in pancreatic cancer but rarely in benign pancreatic diseases. We conducted this study to determine the usefulness of K-ras mutation and telomerase activity in pancreatic juice for the diagnosis of pancreatic cancer.

METHODS

Pancreatic juice collected during endoscopic retrograde cholangiopancreatography was examined in 31 patients: 12 with pancreatic cancer, 11 with chronic pancreatitis, and 8 control patients. The K-ras gene was detected by using the restriction fragment length polymorphism method. Telomerase activity was detected by using the telomeric repeat amplification protocol.

RESULTS

K-ras mutation was positive in 75% (9 of 12) of pancreatic cancers and in 27% (3 of 11) of cases of chronic pancreatitis but in none of the control patients. Telomerase activity was detected in 92% (11 of 12) of pancreatic cancers and in 18% (2 of 11) of cases of chronic pancreatitis. The diagnostic value in pancreatic cancer was comparable between K-ras mutation and telomerase when evaluated separately. However, by combining these 2 methods, the specificity rose to 100%.

CONCLUSIONS

For the diagnosis of pancreatic cancer, telomerase activity in pancreatic juice may possibly be complementary to K-ras mutation because it may decrease the rate of false-positive diagnosis.

Authors+Show Affiliations

Department of Internal Medicine and Institute for Life Science, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10840305

Citation

Myung, S J., et al. "Telomerase Activity in Pure Pancreatic Juice for the Diagnosis of Pancreatic Cancer May Be Complementary to K-ras Mutation." Gastrointestinal Endoscopy, vol. 51, no. 6, 2000, pp. 708-13.
Myung SJ, Kim MH, Kim YS, et al. Telomerase activity in pure pancreatic juice for the diagnosis of pancreatic cancer may be complementary to K-ras mutation. Gastrointest Endosc. 2000;51(6):708-13.
Myung, S. J., Kim, M. H., Kim, Y. S., Kim, H. J., Park, E. T., Yoo, K. S., Lim, B. C., Wan Seo, D., Lee, S. K., Min, Y. I., & Kim, J. Y. (2000). Telomerase activity in pure pancreatic juice for the diagnosis of pancreatic cancer may be complementary to K-ras mutation. Gastrointestinal Endoscopy, 51(6), 708-13.
Myung SJ, et al. Telomerase Activity in Pure Pancreatic Juice for the Diagnosis of Pancreatic Cancer May Be Complementary to K-ras Mutation. Gastrointest Endosc. 2000;51(6):708-13. PubMed PMID: 10840305.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Telomerase activity in pure pancreatic juice for the diagnosis of pancreatic cancer may be complementary to K-ras mutation. AU - Myung,S J, AU - Kim,M H, AU - Kim,Y S, AU - Kim,H J, AU - Park,E T, AU - Yoo,K S, AU - Lim,B C, AU - Wan Seo,D, AU - Lee,S K, AU - Min,Y I, AU - Kim,J Y, PY - 2000/6/7/pubmed PY - 2000/9/2/medline PY - 2000/6/7/entrez SP - 708 EP - 13 JF - Gastrointestinal endoscopy JO - Gastrointest. Endosc. VL - 51 IS - 6 N2 - BACKGROUND: The usefulness of K-ras mutation in pancreatic juice for the diagnosis of pancreatic cancer is questionable. Telomerase is positive in pancreatic cancer but rarely in benign pancreatic diseases. We conducted this study to determine the usefulness of K-ras mutation and telomerase activity in pancreatic juice for the diagnosis of pancreatic cancer. METHODS: Pancreatic juice collected during endoscopic retrograde cholangiopancreatography was examined in 31 patients: 12 with pancreatic cancer, 11 with chronic pancreatitis, and 8 control patients. The K-ras gene was detected by using the restriction fragment length polymorphism method. Telomerase activity was detected by using the telomeric repeat amplification protocol. RESULTS: K-ras mutation was positive in 75% (9 of 12) of pancreatic cancers and in 27% (3 of 11) of cases of chronic pancreatitis but in none of the control patients. Telomerase activity was detected in 92% (11 of 12) of pancreatic cancers and in 18% (2 of 11) of cases of chronic pancreatitis. The diagnostic value in pancreatic cancer was comparable between K-ras mutation and telomerase when evaluated separately. However, by combining these 2 methods, the specificity rose to 100%. CONCLUSIONS: For the diagnosis of pancreatic cancer, telomerase activity in pancreatic juice may possibly be complementary to K-ras mutation because it may decrease the rate of false-positive diagnosis. SN - 0016-5107 UR - https://www.unboundmedicine.com/medline/citation/10840305/Telomerase_activity_in_pure_pancreatic_juice_for_the_diagnosis_of_pancreatic_cancer_may_be_complementary_to_K_ras_mutation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016-5107(00)70083-6 DB - PRIME DP - Unbound Medicine ER -