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Analysis of baclofen by capillary electrophoresis with laser-induced fluorescence detection.
J Chromatogr A. 2000 Apr 28; 877(1-2):233-7.JC

Abstract

A new analytical method for baclofen (4-amino-3-p-chlorophenylbutyric acid) based on capillary electrophoretic separation and laser-induced fluorescence detection has been developed. Naphthalene-2,3-dicarboxaldehyde was used for precolumn derivatization of the non-fluorescent drug. Optimal separation and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH 9.5) and a He-Cd laser (excitation at 442 nm, emission at 500 nm). Linearity (r > or = 0.99) over three orders of magnitude was generally obtained and the concentration limit of detection was in the nanomolar level. Coupled with a simple cleanup procedure, the method was successfully applied to the analysis of baclofen in human plasma. Recovery of spiked baclofen in plasma was 98%. The relative standard deviation values on peak size and migration time were 7.9% and 0.4%, respectively. The limit of detection of baclofen in plasma was 10 ng/ml.

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Authors+Show Affiliations

Chiang MT
Department of Chemistry, Tunghai University, Taichung, Taiwan.
Chang SY
No affiliation info available
Whang CW
No affiliation info available

MeSH

BaclofenElectrophoresis, CapillaryGABA AgonistsHumansLasersSpectrometry, Fluorescence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10845802

Citation

Chiang, M T., et al. "Analysis of Baclofen By Capillary Electrophoresis With Laser-induced Fluorescence Detection." Journal of Chromatography. A, vol. 877, no. 1-2, 2000, pp. 233-7.
Chiang MT, Chang SY, Whang CW. Analysis of baclofen by capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2000;877(1-2):233-7.
Chiang, M. T., Chang, S. Y., & Whang, C. W. (2000). Analysis of baclofen by capillary electrophoresis with laser-induced fluorescence detection. Journal of Chromatography. A, 877(1-2), 233-7.
Chiang MT, Chang SY, Whang CW. Analysis of Baclofen By Capillary Electrophoresis With Laser-induced Fluorescence Detection. J Chromatogr A. 2000 Apr 28;877(1-2):233-7. PubMed PMID: 10845802.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of baclofen by capillary electrophoresis with laser-induced fluorescence detection. AU - Chiang,M T, AU - Chang,S Y, AU - Whang,C W, PY - 2000/6/14/pubmed PY - 2000/9/9/medline PY - 2000/6/14/entrez SP - 233 EP - 7 JF - Journal of chromatography. A JO - J Chromatogr A VL - 877 IS - 1-2 N2 - A new analytical method for baclofen (4-amino-3-p-chlorophenylbutyric acid) based on capillary electrophoretic separation and laser-induced fluorescence detection has been developed. Naphthalene-2,3-dicarboxaldehyde was used for precolumn derivatization of the non-fluorescent drug. Optimal separation and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH 9.5) and a He-Cd laser (excitation at 442 nm, emission at 500 nm). Linearity (r > or = 0.99) over three orders of magnitude was generally obtained and the concentration limit of detection was in the nanomolar level. Coupled with a simple cleanup procedure, the method was successfully applied to the analysis of baclofen in human plasma. Recovery of spiked baclofen in plasma was 98%. The relative standard deviation values on peak size and migration time were 7.9% and 0.4%, respectively. The limit of detection of baclofen in plasma was 10 ng/ml. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/10845802/Analysis_of_baclofen_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(00)00168-0 DB - PRIME DP - Unbound Medicine ER -