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An essential role for a membrane lipid in cytokinesis. Regulation of contractile ring disassembly by redistribution of phosphatidylethanolamine.
J Cell Biol. 2000 Jun 12; 149(6):1215-24.JC

Abstract

Phosphatidylethanolamine (PE) is a major membrane phospholipid that is mainly localized in the inner leaflet of the plasma membrane. We previously demonstrated that PE was exposed on the cell surface of the cleavage furrow during cytokinesis. Immobilization of cell surface PE by a PE-binding peptide inhibited disassembly of the contractile ring components, including myosin II and radixin, resulting in formation of a long cytoplasmic bridge between the daughter cells. This blockade of contractile ring disassembly was reversed by removal of the surface-bound peptide, suggesting that the PE exposure plays a crucial role in cytokinesis. To further examine the role of PE in cytokinesis, we established a mutant cell line with a specific decrease in the cellular PE level. On the culture condition in which the cell surface PE level was significantly reduced, the mutant ceased cell growth in cytokinesis, and the contractile ring remained in the cleavage furrow. Addition of PE or ethanolamine, a precursor of PE synthesis, restored the cell surface PE on the cleavage furrow and normal cytokinesis. These findings provide the first evidence that PE is required for completion of cytokinesis in mammalian cells, and suggest that redistribution of PE on the cleavage furrow may contribute to regulation of contractile ring disassembly.

Authors+Show Affiliations

Department of Molecular Biodynamics, The Tokyo Metropolitan Institute of Medical Science (RINSHOKEN), Bunkyo-ku, Tokyo 113-8613, Japan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10851019

Citation

Emoto, K, and M Umeda. "An Essential Role for a Membrane Lipid in Cytokinesis. Regulation of Contractile Ring Disassembly By Redistribution of Phosphatidylethanolamine." The Journal of Cell Biology, vol. 149, no. 6, 2000, pp. 1215-24.
Emoto K, Umeda M. An essential role for a membrane lipid in cytokinesis. Regulation of contractile ring disassembly by redistribution of phosphatidylethanolamine. J Cell Biol. 2000;149(6):1215-24.
Emoto, K., & Umeda, M. (2000). An essential role for a membrane lipid in cytokinesis. Regulation of contractile ring disassembly by redistribution of phosphatidylethanolamine. The Journal of Cell Biology, 149(6), 1215-24.
Emoto K, Umeda M. An Essential Role for a Membrane Lipid in Cytokinesis. Regulation of Contractile Ring Disassembly By Redistribution of Phosphatidylethanolamine. J Cell Biol. 2000 Jun 12;149(6):1215-24. PubMed PMID: 10851019.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An essential role for a membrane lipid in cytokinesis. Regulation of contractile ring disassembly by redistribution of phosphatidylethanolamine. AU - Emoto,K, AU - Umeda,M, PY - 2000/6/13/pubmed PY - 2000/7/15/medline PY - 2000/6/13/entrez SP - 1215 EP - 24 JF - The Journal of cell biology JO - J. Cell Biol. VL - 149 IS - 6 N2 - Phosphatidylethanolamine (PE) is a major membrane phospholipid that is mainly localized in the inner leaflet of the plasma membrane. We previously demonstrated that PE was exposed on the cell surface of the cleavage furrow during cytokinesis. Immobilization of cell surface PE by a PE-binding peptide inhibited disassembly of the contractile ring components, including myosin II and radixin, resulting in formation of a long cytoplasmic bridge between the daughter cells. This blockade of contractile ring disassembly was reversed by removal of the surface-bound peptide, suggesting that the PE exposure plays a crucial role in cytokinesis. To further examine the role of PE in cytokinesis, we established a mutant cell line with a specific decrease in the cellular PE level. On the culture condition in which the cell surface PE level was significantly reduced, the mutant ceased cell growth in cytokinesis, and the contractile ring remained in the cleavage furrow. Addition of PE or ethanolamine, a precursor of PE synthesis, restored the cell surface PE on the cleavage furrow and normal cytokinesis. These findings provide the first evidence that PE is required for completion of cytokinesis in mammalian cells, and suggest that redistribution of PE on the cleavage furrow may contribute to regulation of contractile ring disassembly. SN - 0021-9525 UR - https://www.unboundmedicine.com/medline/citation/10851019/An_essential_role_for_a_membrane_lipid_in_cytokinesis__Regulation_of_contractile_ring_disassembly_by_redistribution_of_phosphatidylethanolamine_ L2 - https://rupress.org/jcb/article-lookup/doi/10.1083/jcb.149.6.1215 DB - PRIME DP - Unbound Medicine ER -