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Achiral and chiral quantification of methamphetamine and amphetamine in human urine by semi-micro column high-performance liquid chromatography and fluorescence detection.
J Forensic Sci. 2000 May; 45(3):708-14.JF

Abstract

In this paper, miniaturized achiral and chiral high-performance liquid chromatographic procedures for the determination of methamphetamine and amphetamine in human urine are described. After a simple pretreatment of human urine (i.e., 10 microL of urine or diluted urine were acidified and dried-up under N2 at room temperature) and fluorescence derivatization with 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride under mild conditions (pH 9.0, 10 min at room temperature), the derivatives were isocratically separated on a semi-micro ODS column with Tris-HCl buffer (0.1 M, pH 7.0): acetonitrile (45 + 55 v/v) at a flow rate of 0.2 mL/min or their enantiomers were separated on a semi-micro OD-RH column with sodium hexafluorophosphate (0.3 M aq.): acetonitrile (44 + 56 v/v) at a flow rate of 0.1 mL/min as the mobile phase. Wide-ranged calibration curves were obtained with detection limits for the achiral and chiral analyses in the atto and femtomol levels, respectively, per injected volume. Satisfactory within- and between-day reproducibility data were obtained with both the methods with the highest relative standard deviation being 9.6%. The methods were applied to the determination of methamphetamine and amphetamine in human urine samples and the concentrations determined by the two methods were well correlated (r = 0.994).

Authors+Show Affiliations

Department of Analytical Research for Pharmacoinformatics, Graduate School of Pharmaceutical Sciences, Nagasaki University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10855985

Citation

Al-Dirbashi, O Y., et al. "Achiral and Chiral Quantification of Methamphetamine and Amphetamine in Human Urine By Semi-micro Column High-performance Liquid Chromatography and Fluorescence Detection." Journal of Forensic Sciences, vol. 45, no. 3, 2000, pp. 708-14.
Al-Dirbashi OY, Wada M, Kuroda N, et al. Achiral and chiral quantification of methamphetamine and amphetamine in human urine by semi-micro column high-performance liquid chromatography and fluorescence detection. J Forensic Sci. 2000;45(3):708-14.
Al-Dirbashi, O. Y., Wada, M., Kuroda, N., Takahashi, M., & Nakashima, K. (2000). Achiral and chiral quantification of methamphetamine and amphetamine in human urine by semi-micro column high-performance liquid chromatography and fluorescence detection. Journal of Forensic Sciences, 45(3), 708-14.
Al-Dirbashi OY, et al. Achiral and Chiral Quantification of Methamphetamine and Amphetamine in Human Urine By Semi-micro Column High-performance Liquid Chromatography and Fluorescence Detection. J Forensic Sci. 2000;45(3):708-14. PubMed PMID: 10855985.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Achiral and chiral quantification of methamphetamine and amphetamine in human urine by semi-micro column high-performance liquid chromatography and fluorescence detection. AU - Al-Dirbashi,O Y, AU - Wada,M, AU - Kuroda,N, AU - Takahashi,M, AU - Nakashima,K, PY - 2000/6/16/pubmed PY - 2000/7/15/medline PY - 2000/6/16/entrez SP - 708 EP - 14 JF - Journal of forensic sciences JO - J Forensic Sci VL - 45 IS - 3 N2 - In this paper, miniaturized achiral and chiral high-performance liquid chromatographic procedures for the determination of methamphetamine and amphetamine in human urine are described. After a simple pretreatment of human urine (i.e., 10 microL of urine or diluted urine were acidified and dried-up under N2 at room temperature) and fluorescence derivatization with 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride under mild conditions (pH 9.0, 10 min at room temperature), the derivatives were isocratically separated on a semi-micro ODS column with Tris-HCl buffer (0.1 M, pH 7.0): acetonitrile (45 + 55 v/v) at a flow rate of 0.2 mL/min or their enantiomers were separated on a semi-micro OD-RH column with sodium hexafluorophosphate (0.3 M aq.): acetonitrile (44 + 56 v/v) at a flow rate of 0.1 mL/min as the mobile phase. Wide-ranged calibration curves were obtained with detection limits for the achiral and chiral analyses in the atto and femtomol levels, respectively, per injected volume. Satisfactory within- and between-day reproducibility data were obtained with both the methods with the highest relative standard deviation being 9.6%. The methods were applied to the determination of methamphetamine and amphetamine in human urine samples and the concentrations determined by the two methods were well correlated (r = 0.994). SN - 0022-1198 UR - https://www.unboundmedicine.com/medline/citation/10855985/Achiral_and_chiral_quantification_of_methamphetamine_and_amphetamine_in_human_urine_by_semi_micro_column_high_performance_liquid_chromatography_and_fluorescence_detection_ L2 - https://medlineplus.gov/methamphetamine.html DB - PRIME DP - Unbound Medicine ER -