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Effect of cytokines and lipid mediators on the synthesis of interleukin 1 beta by human bone marrow stromal cells.
Cytokine. 2000 May; 12(5):499-502.C

Abstract

This study investigates the production of interleukin (IL-)1beta by cultured human bone marrow stromal cells. RT-PCR experiments indicate that two-thirds of cultures constitutively express IL-1beta mRNA transcripts. Their cell-associated IL-1beta levels are elevated after stimulation with tumour necrosis factor (TNF-)alpha but not with cytokines such as IL-1alpha, IL-3, IL-4, IL-6, IL-7, IL-10, SCF, G-CSF, M-CSF and TGF-beta or lipid mediators such as PGE2, LTB4, LXA4, LXB4, 12-HETE, 15-HETE and PAF. Addition of IL-4, but not IL-10 or TGF-beta, reduces the TNF-alpha-induced cell-associated IL-1beta. IL-1beta is never detected in bone marrow stromal cell supernatants whatever the stimulant added. In conclusion the pro-inflammatory molecule TNF-alpha stimulates bone marrow stromal cell-associated IL-1beta levels while the anti-inflammatory cytokine IL-4 reduces the TNF-alpha-induced effect. These results strengthen the key regulatory role of IL-4 on the production of haematopoietic cytokines by human bone marrow stromal cells.

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Authors+Show Affiliations

Denizot Y
Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, Limoges, France.
Raher S
No affiliation info available
Trimoreau F
No affiliation info available
Praloran V
No affiliation info available
Godard A
No affiliation info available

MeSH

12-Hydroxy-5,8,10,14-eicosatetraenoic AcidBone Marrow CellsCells, CulturedCytokinesDinoprostoneHumansHydroxyeicosatetraenoic AcidsInterleukin-1Leukotriene B4Lipid MetabolismLipoxinsPlatelet Activating FactorStromal Cells

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10857767

Citation

Denizot, Y, et al. "Effect of Cytokines and Lipid Mediators On the Synthesis of Interleukin 1 Beta By Human Bone Marrow Stromal Cells." Cytokine, vol. 12, no. 5, 2000, pp. 499-502.
Denizot Y, Raher S, Trimoreau F, et al. Effect of cytokines and lipid mediators on the synthesis of interleukin 1 beta by human bone marrow stromal cells. Cytokine. 2000;12(5):499-502.
Denizot, Y., Raher, S., Trimoreau, F., Praloran, V., & Godard, A. (2000). Effect of cytokines and lipid mediators on the synthesis of interleukin 1 beta by human bone marrow stromal cells. Cytokine, 12(5), 499-502.
Denizot Y, et al. Effect of Cytokines and Lipid Mediators On the Synthesis of Interleukin 1 Beta By Human Bone Marrow Stromal Cells. Cytokine. 2000;12(5):499-502. PubMed PMID: 10857767.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of cytokines and lipid mediators on the synthesis of interleukin 1 beta by human bone marrow stromal cells. AU - Denizot,Y, AU - Raher,S, AU - Trimoreau,F, AU - Praloran,V, AU - Godard,A, PY - 2000/6/17/pubmed PY - 2000/10/7/medline PY - 2000/6/17/entrez SP - 499 EP - 502 JF - Cytokine JO - Cytokine VL - 12 IS - 5 N2 - This study investigates the production of interleukin (IL-)1beta by cultured human bone marrow stromal cells. RT-PCR experiments indicate that two-thirds of cultures constitutively express IL-1beta mRNA transcripts. Their cell-associated IL-1beta levels are elevated after stimulation with tumour necrosis factor (TNF-)alpha but not with cytokines such as IL-1alpha, IL-3, IL-4, IL-6, IL-7, IL-10, SCF, G-CSF, M-CSF and TGF-beta or lipid mediators such as PGE2, LTB4, LXA4, LXB4, 12-HETE, 15-HETE and PAF. Addition of IL-4, but not IL-10 or TGF-beta, reduces the TNF-alpha-induced cell-associated IL-1beta. IL-1beta is never detected in bone marrow stromal cell supernatants whatever the stimulant added. In conclusion the pro-inflammatory molecule TNF-alpha stimulates bone marrow stromal cell-associated IL-1beta levels while the anti-inflammatory cytokine IL-4 reduces the TNF-alpha-induced effect. These results strengthen the key regulatory role of IL-4 on the production of haematopoietic cytokines by human bone marrow stromal cells. SN - 1043-4666 UR - https://www.unboundmedicine.com/medline/citation/10857767/Effect_of_cytokines_and_lipid_mediators_on_the_synthesis_of_interleukin_1_beta_by_human_bone_marrow_stromal_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1043-4666(99)90578-1 DB - PRIME DP - Unbound Medicine ER -