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Effect of cytokines and growth factors on the macrophage colony-stimulating factor secretion by human bone marrow stromal cells.
Cytokine. 2000 May; 12(5):522-5.C

Abstract

We have investigated the effect of growth factors, inflammatory and anti-inflammatory cytokines on the macrophage colony-stimulating factor (M-CSF) secretion by cultured human bone marrow stromal cells. Their production of M-CSF cultured in serum-free medium is enhanced in a time-dependent manner in response to tumour necrosis factor (TNF-)alpha and interleukin (IL-)4 but not to IL-1, IL-3, IL-6, IL-7, IL-10, SCF, granulocyte-macrophage colony-stimulating factor (GM-CSF), G-CSF, bFGF and transforming growth factor (TGF-)beta. The co-addition of IL-4 and TNF-alpha has a greater than additive effect on the secretion of M-CSF suggesting that they act synergistically. The anti-inflammatory molecules IL-10 and TGF-beta have no effect on the TNF-alpha-induced M-CSF synthesis by marrow stromal cells. In conclusion TNF-alpha and IL-4 are potent stimulators of the M-CSF synthesis by human bone marrow stromal cells, a result of importance regarding the role of M-CSF in the proliferation/differentiation of mononuclear-phagocytic cells and the role of marrow stromal cells as regulators of marrow haematopoiesis.

Authors+Show Affiliations

Besse A
Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, Limoges, France.
Trimoreau F
No affiliation info available
Praloran V
No affiliation info available
Denizot Y
No affiliation info available

MeSH

Bone Marrow CellsCells, CulturedCytokinesGrowth SubstancesHumansMacrophage Colony-Stimulating FactorRNA, MessengerReverse Transcriptase Polymerase Chain ReactionStromal Cells

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10857771

Citation

Besse, A, et al. "Effect of Cytokines and Growth Factors On the Macrophage Colony-stimulating Factor Secretion By Human Bone Marrow Stromal Cells." Cytokine, vol. 12, no. 5, 2000, pp. 522-5.
Besse A, Trimoreau F, Praloran V, et al. Effect of cytokines and growth factors on the macrophage colony-stimulating factor secretion by human bone marrow stromal cells. Cytokine. 2000;12(5):522-5.
Besse, A., Trimoreau, F., Praloran, V., & Denizot, Y. (2000). Effect of cytokines and growth factors on the macrophage colony-stimulating factor secretion by human bone marrow stromal cells. Cytokine, 12(5), 522-5.
Besse A, et al. Effect of Cytokines and Growth Factors On the Macrophage Colony-stimulating Factor Secretion By Human Bone Marrow Stromal Cells. Cytokine. 2000;12(5):522-5. PubMed PMID: 10857771.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of cytokines and growth factors on the macrophage colony-stimulating factor secretion by human bone marrow stromal cells. AU - Besse,A, AU - Trimoreau,F, AU - Praloran,V, AU - Denizot,Y, PY - 2000/6/17/pubmed PY - 2000/10/7/medline PY - 2000/6/17/entrez SP - 522 EP - 5 JF - Cytokine JO - Cytokine VL - 12 IS - 5 N2 - We have investigated the effect of growth factors, inflammatory and anti-inflammatory cytokines on the macrophage colony-stimulating factor (M-CSF) secretion by cultured human bone marrow stromal cells. Their production of M-CSF cultured in serum-free medium is enhanced in a time-dependent manner in response to tumour necrosis factor (TNF-)alpha and interleukin (IL-)4 but not to IL-1, IL-3, IL-6, IL-7, IL-10, SCF, granulocyte-macrophage colony-stimulating factor (GM-CSF), G-CSF, bFGF and transforming growth factor (TGF-)beta. The co-addition of IL-4 and TNF-alpha has a greater than additive effect on the secretion of M-CSF suggesting that they act synergistically. The anti-inflammatory molecules IL-10 and TGF-beta have no effect on the TNF-alpha-induced M-CSF synthesis by marrow stromal cells. In conclusion TNF-alpha and IL-4 are potent stimulators of the M-CSF synthesis by human bone marrow stromal cells, a result of importance regarding the role of M-CSF in the proliferation/differentiation of mononuclear-phagocytic cells and the role of marrow stromal cells as regulators of marrow haematopoiesis. SN - 1043-4666 UR - https://www.unboundmedicine.com/medline/citation/10857771/Effect_of_cytokines_and_growth_factors_on_the_macrophage_colony_stimulating_factor_secretion_by_human_bone_marrow_stromal_cells_ DB - PRIME DP - Unbound Medicine ER -