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Determination of Ibuprofen in human plasma by high-performance liquid chromatography: validation and application in pharmacokinetic study.
Biomed Chromatogr. 2000 Jun; 14(4):219-26.BC

Abstract

A specific method for the simultaneous determination of S-(+)Ibuprofen and R-(-)Ibuprofen enantiomers in human plasma is described. Adopting a high-performance liquid chromatographic (HPLC) system with spectrofluorometer detector, the compounds were extracted from plasma in alcohol medium and were separated on C18 column, using a solution of acetonitrile-water-acetic acid-triethylamine as mobile phase. The limit of quantitation was 0.1 microg/mL for both compounds. The method was validated by intra-day assays at three concentration levels and was used in a kinetic study in healthy volunteers. During the study we carried out inter-day assays to confirm the feasibility of the method.

Authors+Show Affiliations

Department of Anatomy, Pharmacology and Forensic Medicine, University of Torino, Torino, Italy. canaparo@medfarm.unito.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10861732

Citation

Canaparo, R, et al. "Determination of Ibuprofen in Human Plasma By High-performance Liquid Chromatography: Validation and Application in Pharmacokinetic Study." Biomedical Chromatography : BMC, vol. 14, no. 4, 2000, pp. 219-26.
Canaparo R, Muntoni E, Zara GP, et al. Determination of Ibuprofen in human plasma by high-performance liquid chromatography: validation and application in pharmacokinetic study. Biomed Chromatogr. 2000;14(4):219-26.
Canaparo, R., Muntoni, E., Zara, G. P., Della Pepa, C., Berno, E., Costa, M., & Eandi, M. (2000). Determination of Ibuprofen in human plasma by high-performance liquid chromatography: validation and application in pharmacokinetic study. Biomedical Chromatography : BMC, 14(4), 219-26.
Canaparo R, et al. Determination of Ibuprofen in Human Plasma By High-performance Liquid Chromatography: Validation and Application in Pharmacokinetic Study. Biomed Chromatogr. 2000;14(4):219-26. PubMed PMID: 10861732.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of Ibuprofen in human plasma by high-performance liquid chromatography: validation and application in pharmacokinetic study. AU - Canaparo,R, AU - Muntoni,E, AU - Zara,G P, AU - Della Pepa,C, AU - Berno,E, AU - Costa,M, AU - Eandi,M, PY - 2000/6/22/pubmed PY - 2000/7/25/medline PY - 2000/6/22/entrez SP - 219 EP - 26 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 14 IS - 4 N2 - A specific method for the simultaneous determination of S-(+)Ibuprofen and R-(-)Ibuprofen enantiomers in human plasma is described. Adopting a high-performance liquid chromatographic (HPLC) system with spectrofluorometer detector, the compounds were extracted from plasma in alcohol medium and were separated on C18 column, using a solution of acetonitrile-water-acetic acid-triethylamine as mobile phase. The limit of quantitation was 0.1 microg/mL for both compounds. The method was validated by intra-day assays at three concentration levels and was used in a kinetic study in healthy volunteers. During the study we carried out inter-day assays to confirm the feasibility of the method. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/10861732/Determination_of_Ibuprofen_in_human_plasma_by_high_performance_liquid_chromatography:_validation_and_application_in_pharmacokinetic_study_ DB - PRIME DP - Unbound Medicine ER -