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The importance of ATP as a regulator of glycolytic flux in Saccharomyces cerevisiae.
Yeast. 2000 Jun 30; 16(9):797-809.Y

Abstract

The control of glycolytic flux in the yeast Saccharomyces cerevisiae was studied by using permeabilized cells. Cells were harvested from chemostat cultures and, after removal of the cell wall, nystatin was used to permeabilize the spheroplasts. By this method it is possible to study the performance and regulation of a complete and functional metabolic pathway and not only a single enzymatic step. The results showed that ATP has a strong negative effect on glycolytic activity affecting several of the glycolytic enzymes. However, the main targets for ATP inhibition was phosphofructokinase and pyruvate kinase. Phospofructokinase was inhibited by ATP concentrations starting at about 1-2 mM, while pyruvate kinase required ATP levels above 2.5 mM before any inhibition was visible. These ATP concentrations were in the same range as measured for nitrogen- and glucose-limited cells cultivated in chemostat cultures. Other potential candidates as enzymes susceptible to ATP inhibition included hexokinase and enolase. The ATP:ADP ratio, as well as trehalose-6-phosphate levels, did not seem to influence the glycolytic activity.

Authors+Show Affiliations

Larsson C
Department of Molecular Biotechnology, Chalmers University of Technology, Box 462, S-405 30 Göteborg, Sweden. Christer.Larsson@gmm.gu.se
Påhlman IL
No affiliation info available
Gustafsson L
No affiliation info available

MeSH

Adenosine DiphosphateAdenosine TriphosphateAnaerobiosisCell Membrane PermeabilityCulture MediaGlucoseGlycolysisNADNitrogenSaccharomyces cerevisiaeSubstrate SpecificitySugar PhosphatesTrehalose

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10861904

Citation

Larsson, C, et al. "The Importance of ATP as a Regulator of Glycolytic Flux in Saccharomyces Cerevisiae." Yeast (Chichester, England), vol. 16, no. 9, 2000, pp. 797-809.
Larsson C, Påhlman IL, Gustafsson L. The importance of ATP as a regulator of glycolytic flux in Saccharomyces cerevisiae. Yeast. 2000;16(9):797-809.
Larsson, C., Påhlman, I. L., & Gustafsson, L. (2000). The importance of ATP as a regulator of glycolytic flux in Saccharomyces cerevisiae. Yeast (Chichester, England), 16(9), 797-809.
Larsson C, Påhlman IL, Gustafsson L. The Importance of ATP as a Regulator of Glycolytic Flux in Saccharomyces Cerevisiae. Yeast. 2000 Jun 30;16(9):797-809. PubMed PMID: 10861904.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The importance of ATP as a regulator of glycolytic flux in Saccharomyces cerevisiae. AU - Larsson,C, AU - Påhlman,I L, AU - Gustafsson,L, PY - 2000/6/22/pubmed PY - 2000/10/7/medline PY - 2000/6/22/entrez SP - 797 EP - 809 JF - Yeast (Chichester, England) JO - Yeast VL - 16 IS - 9 N2 - The control of glycolytic flux in the yeast Saccharomyces cerevisiae was studied by using permeabilized cells. Cells were harvested from chemostat cultures and, after removal of the cell wall, nystatin was used to permeabilize the spheroplasts. By this method it is possible to study the performance and regulation of a complete and functional metabolic pathway and not only a single enzymatic step. The results showed that ATP has a strong negative effect on glycolytic activity affecting several of the glycolytic enzymes. However, the main targets for ATP inhibition was phosphofructokinase and pyruvate kinase. Phospofructokinase was inhibited by ATP concentrations starting at about 1-2 mM, while pyruvate kinase required ATP levels above 2.5 mM before any inhibition was visible. These ATP concentrations were in the same range as measured for nitrogen- and glucose-limited cells cultivated in chemostat cultures. Other potential candidates as enzymes susceptible to ATP inhibition included hexokinase and enolase. The ATP:ADP ratio, as well as trehalose-6-phosphate levels, did not seem to influence the glycolytic activity. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/10861904/The_importance_of_ATP_as_a_regulator_of_glycolytic_flux_in_Saccharomyces_cerevisiae_ L2 - https://doi.org/10.1002/1097-0061(20000630)16:9<797::AID-YEA553>3.0.CO;2-5 DB - PRIME DP - Unbound Medicine ER -