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A tandem quadrupole/time-of-flight mass spectrometer with a matrix-assisted laser desorption/ionization source: design and performance
Rapid Commun Mass Spectrom. 2000; 14(12):1047-57.RC

Abstract

A matrix-assisted laser desorption/ionization (MALDI) source has been coupled to a tandem quadrupole/time-of-flight (QqTOF) mass spectrometer by means of a collisional damping interface. Mass resolving power of about 10,000 (FWHM) and accuracy in the range of 10 ppm are observed in both single-MS mode and MS/MS mode. Sub-femtomole sensitivity is obtained in single-MS mode, and a few femtomoles in MS/MS mode. Both peptide mass mapping and collision-induced dissociation (CID) analysis of tryptic peptides can be performed from the same MALDI target. Rapid spectral acquisition (a few seconds per spectrum) can be achieved in both modes, so high throughput protein identification is possible. Some information about fragmentation patterns was obtained from a study of the CID spectra of singly charged peptides from a tryptic digest of E. coli citrate synthase. Reasonably successful automatic sequence prediction (>90%) is possible from the CID spectra of singly charged peptides using the SCIEX Predict Sequence routine. Ion production at pressures near 1 Torr (rather than in vacuum) is found to give reduced metastable fragmentation, particularly for higher mass molecular ions. Copyright 2000 John Wiley & Sons, Ltd.

Authors+Show Affiliations

Department of Physics and Astronomy, University of Manitoba, Winnipeg MB R3T2N2, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10861986

Citation

Loboda, AV, et al. "A Tandem Quadrupole/time-of-flight Mass Spectrometer With a Matrix-assisted Laser Desorption/ionization Source: Design and Performance." Rapid Communications in Mass Spectrometry : RCM, vol. 14, no. 12, 2000, pp. 1047-57.
Loboda AV, Krutchinsky AN, Bromirski M, et al. A tandem quadrupole/time-of-flight mass spectrometer with a matrix-assisted laser desorption/ionization source: design and performance. Rapid Commun Mass Spectrom. 2000;14(12):1047-57.
Loboda, A. V., Krutchinsky, A. N., Bromirski, M., Ens, W., & Standing, K. G. (2000). A tandem quadrupole/time-of-flight mass spectrometer with a matrix-assisted laser desorption/ionization source: design and performance. Rapid Communications in Mass Spectrometry : RCM, 14(12), 1047-57.
Loboda AV, et al. A Tandem Quadrupole/time-of-flight Mass Spectrometer With a Matrix-assisted Laser Desorption/ionization Source: Design and Performance. Rapid Commun Mass Spectrom. 2000;14(12):1047-57. PubMed PMID: 10861986.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A tandem quadrupole/time-of-flight mass spectrometer with a matrix-assisted laser desorption/ionization source: design and performance AU - Loboda,AV, AU - Krutchinsky,AN, AU - Bromirski,M, AU - Ens,W, AU - Standing,KG, PY - 2000/6/22/pubmed PY - 2000/6/22/medline PY - 2000/6/22/entrez SP - 1047 EP - 57 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 14 IS - 12 N2 - A matrix-assisted laser desorption/ionization (MALDI) source has been coupled to a tandem quadrupole/time-of-flight (QqTOF) mass spectrometer by means of a collisional damping interface. Mass resolving power of about 10,000 (FWHM) and accuracy in the range of 10 ppm are observed in both single-MS mode and MS/MS mode. Sub-femtomole sensitivity is obtained in single-MS mode, and a few femtomoles in MS/MS mode. Both peptide mass mapping and collision-induced dissociation (CID) analysis of tryptic peptides can be performed from the same MALDI target. Rapid spectral acquisition (a few seconds per spectrum) can be achieved in both modes, so high throughput protein identification is possible. Some information about fragmentation patterns was obtained from a study of the CID spectra of singly charged peptides from a tryptic digest of E. coli citrate synthase. Reasonably successful automatic sequence prediction (>90%) is possible from the CID spectra of singly charged peptides using the SCIEX Predict Sequence routine. Ion production at pressures near 1 Torr (rather than in vacuum) is found to give reduced metastable fragmentation, particularly for higher mass molecular ions. Copyright 2000 John Wiley & Sons, Ltd. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/10861986/A_tandem_quadrupole/time_of_flight_mass_spectrometer_with_a_matrix_assisted_laser_desorption/ionization_source:_design_and_performance L2 - https://doi.org/10.1002/1097-0231(20000630)14:12<1047::AID-RCM990>3.0.CO;2-E DB - PRIME DP - Unbound Medicine ER -
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