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The spacing between functional Cis-elements of U3 snoRNA is critical for rRNA processing.
J Mol Biol. 2000 Jun 30; 300(1):57-74.JM

Abstract

The sequences and structural features of Xenopus laevis U3 small nucleolar RNA (snoRNA) necessary for pre-rRNA cleavage at sites 1 and 2 to form 18 S rRNA were assayed by depletion/rescue experiments in Xenopus oocytes. Mutagenesis results demonstrated that the putative stem of U3 domain I is unnecessary for 18 S rRNA processing. A model consistent with earlier experimental data is proposed for the structure of domain I when U3 is not yet bound to pre-rRNA. For its function in rRNA processing, a newly discovered element (5' hinge) was revealed to be important but not as critical as the 3' hinge region in Xenopus U3 snoRNA for 18 S rRNA formation. Base-pairing is proposed to occur between the U3 5' hinge and 3' hinge and complementary regions in the external transcribed spacer (ETS); these interactions are phylogenetically conserved, and are homologous to those previously described in yeast (5' hinge-ETS) and trypanosomes (3' hinge-ETS). A model is presented where the base-pairing of the 5' hinge and 3' hinge of U3 snoRNA with the ETS of pre-rRNA helps to correctly position U3 boxes A'+A for their function in rRNA processing. Like an earlier proposal for yeast, boxes A' and A of Xenopus may base-pair with 18 S sequences in pre-rRNA. We present the first direct experimental evidence in any system that box A' is essential for U3 snoRNA function in 18 S rRNA formation. The analysis of insertions and deletions indicated that the spacing between the U3 elements is important, suggesting that they base-pair with the ETS and 18 S regions of pre-rRNA at the same time.

Authors+Show Affiliations

Division of Biology and Medicine, Brown University, Providence, RI, 02912, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10864498

Citation

Borovjagin, A V., and S A. Gerbi. "The Spacing Between Functional Cis-elements of U3 snoRNA Is Critical for rRNA Processing." Journal of Molecular Biology, vol. 300, no. 1, 2000, pp. 57-74.
Borovjagin AV, Gerbi SA. The spacing between functional Cis-elements of U3 snoRNA is critical for rRNA processing. J Mol Biol. 2000;300(1):57-74.
Borovjagin, A. V., & Gerbi, S. A. (2000). The spacing between functional Cis-elements of U3 snoRNA is critical for rRNA processing. Journal of Molecular Biology, 300(1), 57-74.
Borovjagin AV, Gerbi SA. The Spacing Between Functional Cis-elements of U3 snoRNA Is Critical for rRNA Processing. J Mol Biol. 2000 Jun 30;300(1):57-74. PubMed PMID: 10864498.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The spacing between functional Cis-elements of U3 snoRNA is critical for rRNA processing. AU - Borovjagin,A V, AU - Gerbi,S A, PY - 2000/6/23/pubmed PY - 2000/8/1/medline PY - 2000/6/23/entrez SP - 57 EP - 74 JF - Journal of molecular biology JO - J Mol Biol VL - 300 IS - 1 N2 - The sequences and structural features of Xenopus laevis U3 small nucleolar RNA (snoRNA) necessary for pre-rRNA cleavage at sites 1 and 2 to form 18 S rRNA were assayed by depletion/rescue experiments in Xenopus oocytes. Mutagenesis results demonstrated that the putative stem of U3 domain I is unnecessary for 18 S rRNA processing. A model consistent with earlier experimental data is proposed for the structure of domain I when U3 is not yet bound to pre-rRNA. For its function in rRNA processing, a newly discovered element (5' hinge) was revealed to be important but not as critical as the 3' hinge region in Xenopus U3 snoRNA for 18 S rRNA formation. Base-pairing is proposed to occur between the U3 5' hinge and 3' hinge and complementary regions in the external transcribed spacer (ETS); these interactions are phylogenetically conserved, and are homologous to those previously described in yeast (5' hinge-ETS) and trypanosomes (3' hinge-ETS). A model is presented where the base-pairing of the 5' hinge and 3' hinge of U3 snoRNA with the ETS of pre-rRNA helps to correctly position U3 boxes A'+A for their function in rRNA processing. Like an earlier proposal for yeast, boxes A' and A of Xenopus may base-pair with 18 S sequences in pre-rRNA. We present the first direct experimental evidence in any system that box A' is essential for U3 snoRNA function in 18 S rRNA formation. The analysis of insertions and deletions indicated that the spacing between the U3 elements is important, suggesting that they base-pair with the ETS and 18 S regions of pre-rRNA at the same time. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/10864498/The_spacing_between_functional_Cis_elements_of_U3_snoRNA_is_critical_for_rRNA_processing_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(00)93798-7 DB - PRIME DP - Unbound Medicine ER -