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Role of the Salmonella abortusovis virulence plasmid in the infection of BALB/c mice.
FEMS Microbiol Lett. 2000 Jul 01; 188(1):15-8.FM

Abstract

Following oral inoculation of BALB/c mice, Salmonella abortusovis strain SS44 was recovered in lower numbers from the Peyer's patches and mesenteric lymph nodes compared with S. typhimurium strain SL1344, whereas splenic infections were equivalent between the two serovars. SS44 was cured of its virulence plasmid or subjected to mutagenesis of the spv genes, and the Spv(-) derivatives were tested for virulence in mice. Plasmid-cured S. abortusovis SU40 retained virulence in BALB/c mice when inoculated intraperitoneally. On the other hand, mice infected orally with SU40 had greatly reduced splenic infection compared to those infected with wild-type SS44. Similar results were obtained after Tn5 insertion mutagenesis of the spvR gene or deletion of the spvABCD locus. These results suggest that in the gut-associated lymphoid tissues S. abortusovis may replicate less than S. typhimurium and that the S. abortusovis virulence plasmid primarily affects systemic infection after oral inoculation but not after intraperitoneal administration in the mouse model.

Authors+Show Affiliations

Department of Biomedical Science, University of Sassari, Italy. uzzua@ssmain.uniss.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10867227

Citation

Uzzau, S, et al. "Role of the Salmonella Abortusovis Virulence Plasmid in the Infection of BALB/c Mice." FEMS Microbiology Letters, vol. 188, no. 1, 2000, pp. 15-8.
Uzzau S, Gulig PA, Paglietti B, et al. Role of the Salmonella abortusovis virulence plasmid in the infection of BALB/c mice. FEMS Microbiol Lett. 2000;188(1):15-8.
Uzzau, S., Gulig, P. A., Paglietti, B., Leori, G., Stocker, B. A., & Rubino, S. (2000). Role of the Salmonella abortusovis virulence plasmid in the infection of BALB/c mice. FEMS Microbiology Letters, 188(1), 15-8.
Uzzau S, et al. Role of the Salmonella Abortusovis Virulence Plasmid in the Infection of BALB/c Mice. FEMS Microbiol Lett. 2000 Jul 1;188(1):15-8. PubMed PMID: 10867227.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of the Salmonella abortusovis virulence plasmid in the infection of BALB/c mice. AU - Uzzau,S, AU - Gulig,P A, AU - Paglietti,B, AU - Leori,G, AU - Stocker,B A, AU - Rubino,S, PY - 2000/6/27/pubmed PY - 2000/9/19/medline PY - 2000/6/27/entrez SP - 15 EP - 8 JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 188 IS - 1 N2 - Following oral inoculation of BALB/c mice, Salmonella abortusovis strain SS44 was recovered in lower numbers from the Peyer's patches and mesenteric lymph nodes compared with S. typhimurium strain SL1344, whereas splenic infections were equivalent between the two serovars. SS44 was cured of its virulence plasmid or subjected to mutagenesis of the spv genes, and the Spv(-) derivatives were tested for virulence in mice. Plasmid-cured S. abortusovis SU40 retained virulence in BALB/c mice when inoculated intraperitoneally. On the other hand, mice infected orally with SU40 had greatly reduced splenic infection compared to those infected with wild-type SS44. Similar results were obtained after Tn5 insertion mutagenesis of the spvR gene or deletion of the spvABCD locus. These results suggest that in the gut-associated lymphoid tissues S. abortusovis may replicate less than S. typhimurium and that the S. abortusovis virulence plasmid primarily affects systemic infection after oral inoculation but not after intraperitoneal administration in the mouse model. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/10867227/Role_of_the_Salmonella_abortusovis_virulence_plasmid_in_the_infection_of_BALB/c_mice_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.2000.tb09161.x DB - PRIME DP - Unbound Medicine ER -