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Genetic diversity of the Junin virus in Argentina: geographic and temporal patterns.
Virology. 2000 Jun 20; 272(1):127-36.V

Abstract

RNA was purified from 39 strains of cell-cultured Junin virus (JUN) from central Argentina, which included both human- and rodent-derived isolates (a total of 26 and 13, respectively), as well as from 2 laboratory JUN strains, XJ Cl3 and XJ #44. JUN-specific primers were used to amplify a 511-nucleotide (nt) fragment of the nucleocapsid protein gene and a 495-nt fragment of the glycoprotein 1 (GP1) gene. Genetic diversity among JUN strains studied was up to 13% at the nt level and up to 9% at the amino acid (aa) level for the GP1 gene and up to 9% (nt) and 4% (aa) for the NP gene. Phylogenetic analyses of both genes revealed three distinct clades. The first clade was composed of the JUN strains from the center of the endemic area and included the majority of JUN strains analyzed in the current study. The second clade contained 4 JUN strains isolated between 1963 and 1971 from Cordoba Province, the western-most edge of the known endemic area. The third clade contained 4 JUN strains that originated from Calomys musculinus trapped in Zarate, the northeastern edge of the known endemic area. Certain JUN sequences, which were obtained from GenBank and identified as XJ, XJ #44, and Candid #1 strains, appeared to form a separate clade. Over 400 nt of the GP1 and GP2 genes were additionally sequenced for 7 JUN strains derived from patients with different clinical presentations and outcomes of Argentine hemorrhagic fever. Analysis of the corresponding aa sequences did not allow us to attribute any particular genetic marker to the changing severity or clinical form of the human disease.

Authors+Show Affiliations

Instituto Nacional de Enfermedades Virales Humanas, Pergamino, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10873755

Citation

García, J B., et al. "Genetic Diversity of the Junin Virus in Argentina: Geographic and Temporal Patterns." Virology, vol. 272, no. 1, 2000, pp. 127-36.
García JB, Morzunov SP, Levis S, et al. Genetic diversity of the Junin virus in Argentina: geographic and temporal patterns. Virology. 2000;272(1):127-36.
García, J. B., Morzunov, S. P., Levis, S., Rowe, J., Calderón, G., Enría, D., Sabattini, M., Buchmeier, M. J., Bowen, M. D., & St Jeor, S. C. (2000). Genetic diversity of the Junin virus in Argentina: geographic and temporal patterns. Virology, 272(1), 127-36.
García JB, et al. Genetic Diversity of the Junin Virus in Argentina: Geographic and Temporal Patterns. Virology. 2000 Jun 20;272(1):127-36. PubMed PMID: 10873755.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic diversity of the Junin virus in Argentina: geographic and temporal patterns. AU - García,J B, AU - Morzunov,S P, AU - Levis,S, AU - Rowe,J, AU - Calderón,G, AU - Enría,D, AU - Sabattini,M, AU - Buchmeier,M J, AU - Bowen,M D, AU - St Jeor,S C, PY - 2000/6/30/pubmed PY - 2000/7/25/medline PY - 2000/6/30/entrez SP - 127 EP - 36 JF - Virology JO - Virology VL - 272 IS - 1 N2 - RNA was purified from 39 strains of cell-cultured Junin virus (JUN) from central Argentina, which included both human- and rodent-derived isolates (a total of 26 and 13, respectively), as well as from 2 laboratory JUN strains, XJ Cl3 and XJ #44. JUN-specific primers were used to amplify a 511-nucleotide (nt) fragment of the nucleocapsid protein gene and a 495-nt fragment of the glycoprotein 1 (GP1) gene. Genetic diversity among JUN strains studied was up to 13% at the nt level and up to 9% at the amino acid (aa) level for the GP1 gene and up to 9% (nt) and 4% (aa) for the NP gene. Phylogenetic analyses of both genes revealed three distinct clades. The first clade was composed of the JUN strains from the center of the endemic area and included the majority of JUN strains analyzed in the current study. The second clade contained 4 JUN strains isolated between 1963 and 1971 from Cordoba Province, the western-most edge of the known endemic area. The third clade contained 4 JUN strains that originated from Calomys musculinus trapped in Zarate, the northeastern edge of the known endemic area. Certain JUN sequences, which were obtained from GenBank and identified as XJ, XJ #44, and Candid #1 strains, appeared to form a separate clade. Over 400 nt of the GP1 and GP2 genes were additionally sequenced for 7 JUN strains derived from patients with different clinical presentations and outcomes of Argentine hemorrhagic fever. Analysis of the corresponding aa sequences did not allow us to attribute any particular genetic marker to the changing severity or clinical form of the human disease. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/10873755/Genetic_diversity_of_the_Junin_virus_in_Argentina:_geographic_and_temporal_patterns_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042682200903453 DB - PRIME DP - Unbound Medicine ER -