Tags

Type your tag names separated by a space and hit enter

Evaluation of a commercially available recombinant-protein enzyme-linked immunosorbent assay for detection of antibodies produced in scrub typhus rickettsial infections.
J Clin Microbiol. 2000 Jul; 38(7):2701-5.JC

Abstract

The 56-kDa major outer membrane protein antigen of Orientia tsutsugamuchi is the immunodominant antigen in human scrub typhus (ST) infections. An enzyme-linked immunosorbent assay (ELISA) using a recombinant 56-kDa protein (r56) to detect specific immunoglobulin M (IgM) produced in ST infections was developed, and its performance was evaluated using sera from patients with active ST (n = 59), spotted fever (SF) (n = 31), and murine typhus (MT) (n = 6) and from those without rickettsial infection (n = 52). The r56 ELISA was compared to an ELISA using native whole cell lysate of O. tsutsugamushi Karp or O. tsutsugamushi Gilliam as antigens. The performance of the assays using r56 was similar to that of those using native antigens. Using indirect immunoperoxidase (IIP) as the reference test, sensitivities were 86, 88, and 88% while specificities were 84, 90, and 87% in the three assays. Furthermore, cross-reactivity in confirmed cases of SF and MT was low (5.4, 2.7, and 2.7% respectively). The additional use of IgG in the r56 ELISA gave improved performance (sensitivity, 80%; specificity, 96%; cross-reactivity in SF and MT, 2.7%). The detection of high levels of IgG in some IgM-negative patients illustrates the importance of including a test for IgG in the detection of secondary or reactivated infections, since many of these patients were from regions in Thailand where these infections are endemic.

Links

PMC Free PDF
PMC Free Full Text
FREE Publisher Full Text

Authors+Show Affiliations

Land MV
PanBio Pty. Ltd., Windsor, Queensland, Australia.
Ching WM
No affiliation info available
Dasch GA
No affiliation info available
Zhang Z
No affiliation info available
Kelly DJ
No affiliation info available
Graves SR
No affiliation info available
Devine PL
No affiliation info available

MeSH

Antibodies, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsCross ReactionsEnzyme-Linked Immunosorbent AssayEvaluation Studies as TopicHumansImmunoenzyme TechniquesOrientia tsutsugamushiReagent Kits, DiagnosticRecombinant ProteinsRickettsia InfectionsScrub TyphusSensitivity and Specificity

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10878067

Citation

Land, M V., et al. "Evaluation of a Commercially Available Recombinant-protein Enzyme-linked Immunosorbent Assay for Detection of Antibodies Produced in Scrub Typhus Rickettsial Infections." Journal of Clinical Microbiology, vol. 38, no. 7, 2000, pp. 2701-5.
Land MV, Ching WM, Dasch GA, et al. Evaluation of a commercially available recombinant-protein enzyme-linked immunosorbent assay for detection of antibodies produced in scrub typhus rickettsial infections. J Clin Microbiol. 2000;38(7):2701-5.
Land, M. V., Ching, W. M., Dasch, G. A., Zhang, Z., Kelly, D. J., Graves, S. R., & Devine, P. L. (2000). Evaluation of a commercially available recombinant-protein enzyme-linked immunosorbent assay for detection of antibodies produced in scrub typhus rickettsial infections. Journal of Clinical Microbiology, 38(7), 2701-5.
Land MV, et al. Evaluation of a Commercially Available Recombinant-protein Enzyme-linked Immunosorbent Assay for Detection of Antibodies Produced in Scrub Typhus Rickettsial Infections. J Clin Microbiol. 2000;38(7):2701-5. PubMed PMID: 10878067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of a commercially available recombinant-protein enzyme-linked immunosorbent assay for detection of antibodies produced in scrub typhus rickettsial infections. AU - Land,M V, AU - Ching,W M, AU - Dasch,G A, AU - Zhang,Z, AU - Kelly,D J, AU - Graves,S R, AU - Devine,P L, PY - 2000/7/6/pubmed PY - 2000/9/9/medline PY - 2000/7/6/entrez SP - 2701 EP - 5 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 38 IS - 7 N2 - The 56-kDa major outer membrane protein antigen of Orientia tsutsugamuchi is the immunodominant antigen in human scrub typhus (ST) infections. An enzyme-linked immunosorbent assay (ELISA) using a recombinant 56-kDa protein (r56) to detect specific immunoglobulin M (IgM) produced in ST infections was developed, and its performance was evaluated using sera from patients with active ST (n = 59), spotted fever (SF) (n = 31), and murine typhus (MT) (n = 6) and from those without rickettsial infection (n = 52). The r56 ELISA was compared to an ELISA using native whole cell lysate of O. tsutsugamushi Karp or O. tsutsugamushi Gilliam as antigens. The performance of the assays using r56 was similar to that of those using native antigens. Using indirect immunoperoxidase (IIP) as the reference test, sensitivities were 86, 88, and 88% while specificities were 84, 90, and 87% in the three assays. Furthermore, cross-reactivity in confirmed cases of SF and MT was low (5.4, 2.7, and 2.7% respectively). The additional use of IgG in the r56 ELISA gave improved performance (sensitivity, 80%; specificity, 96%; cross-reactivity in SF and MT, 2.7%). The detection of high levels of IgG in some IgM-negative patients illustrates the importance of including a test for IgG in the detection of secondary or reactivated infections, since many of these patients were from regions in Thailand where these infections are endemic. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/10878067/Evaluation_of_a_commercially_available_recombinant_protein_enzyme_linked_immunosorbent_assay_for_detection_of_antibodies_produced_in_scrub_typhus_rickettsial_infections_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=10878067 DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓16 ↑14]

Related Citations

More